Simplified procedure for the detection of antigens by crossed immunoelectrophoresis.

Keyser, J. W.; Lewis, K

doi:10.1136/jcp.26.2.160

Cited by 2 publications

(1 citation statement)

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“…From Table 1 it is obvious that some antisera against human proteins did not cross-react with the animal sera tested. However, by using electroimmunodiffusion with triangular troughs for antigen and antiserum as described by KEYSER et al (1973) precipitates could be visible between i. a. anti-human a,-antitrypsin and swine serum (Martinsson, unpublished). Such precipitates were not observed after immunoelectrophoresis or immunodiffusion.…”

Section: Discussionmentioning

confidence: 99%

Preparation of Antisera against Different Animal Serum Proteins. using Cross-reacting Specific Anti-human Sera

Martinsson

2010

Zentralblatt Für Veterinärmedizin Reihe B

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Summary Cross‐reactions between specific antisera against different human serum proteins and sera from swine, horse and dog were studied. Such cross‐reactions were used for preparation of agar‐gel precipitates which were used for immunizations in rabbits. The precipitates were injected into enlarged lymphnodes and in this way specific antisera against swine ceruloplasmin and haptoglobin were obtained. The practical importance of the methods is discussed. Zusammenfassung Es wurden die Kreuzreaktionen zwischen spezifischen Antiseren gegen verschiedene menschliche Serumproteine und Seren von Schwein, Pferd und Hund untersucht. Diese Kreuzreaktionen wurden für die Präparation von Agargel‐Präzipitaten genutzt, die für die Immunisierung von Kaninchen verwendet wurden. Durch Injektion der Präzipitate in vergrößerte Lymphknoten ließen sich spezifische Antiseren gegen Schweine‐Ceruloplasmin und ‐Haptoglobin herstellen. Die praktische Bedeutung der Methoden wird diskutiert.

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Section: Discussionmentioning

confidence: 99%