Simulation of ECM with silk and chitosan nanocomposite materials

Ding, Zhaozhao; Ma, Jiliang; He, Wenya; Zhang, Ge; Lü, Qiang; Kaplan, David L.

doi:10.1039/c7tb00486a

Cited by 24 publications

(20 citation statements)

References 72 publications

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“…These changes suggested that further assembly occurred during the incubation procedure and freezing process at higher temperature, resulting in more nanofiber formation. Similar to acid-assisted RSF scaffold formation, the extracellular matrix-mimetic nanofibrous structure assembled in the TS scaffolds, which would provide favorable cues for cell growth and proliferation (Calle et al, 2016;Ding et al, 2017;Gu et al, 2014;Han et al, 2016). Flt-1 CGGAGAAATCTGCTC GCTATCTTGGAAGGGA CGACACG attributed to amorphous states (Bai et al, 2015;Sang et al, 2018;Wang et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Water‐insoluble amorphous silk fibroin scaffolds from aqueous solutions

Fan

Xiao

et al. 2019

J Biomed Mater Res

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Regenerated silk fibroin (RSF) is emerging as promising biomaterial for regeneration, drug delivery and optical devices, with continued demand for mild, all‐aqueous processes to control microstructure and the performance. Here, temperature control of assembly kinetics was introduced to prepare the water‐insoluble scaffolds from neutral aqueous solutions of RSF protein. Higher temperatures were used to accelerate the assembly rate of the silk fibroin protein chains in aqueous solution and during the lyophilization process, resulting in water‐insoluble scaffold formation. The scaffolds were mainly composed of amorphous states of the silk fibroin chains, endowing softer mechanical properties. These scaffolds also showed nanofibrous structures, improved cell proliferation in vitro and enhanced neovascularization and tissue regeneration in vivo than previously reported silk fibroin scaffolds. These results suggest utility of silk scaffolds in soft tissue regeneration.

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Section: Methodsmentioning

confidence: 99%

Water‐insoluble amorphous silk fibroin scaffolds from aqueous solutions

Fan

Xiao

et al. 2019

J Biomed Mater Res

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“…Some applications include engineering the extracellular matrix (ECM), wound healing applications, cardiac tissue regeneration, bone regeneration, or liver tissue engineering. Ding et al fabricated natural scaffolds to simulate the ECM using silk fibroin and chitosan for future applications in tissue regeneration [120]. The nanostructures demonstrated an improvement of biocompatibility, cell proliferation, and neovascularization.…”

Section: Tissue Regenerationmentioning

confidence: 99%

Protein–Polysaccharide Composite Materials: Fabrication and Applications

et al. 2020

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Protein–polysaccharide composites have been known to show a wide range of applications in biomedical and green chemical fields. These composites have been fabricated into a variety of forms, such as films, fibers, particles, and gels, dependent upon their specific applications. Post treatments of these composites, such as enhancing chemical and physical changes, have been shown to favorably alter their structure and properties, allowing for specificity of medical treatments. Protein–polysaccharide composite materials introduce many opportunities to improve biological functions and contemporary technological functions. Current applications involving the replication of artificial tissues in tissue regeneration, wound therapy, effective drug delivery systems, and food colloids have benefited from protein–polysaccharide composite materials. Although there is limited research on the development of protein–polysaccharide composites, studies have proven their effectiveness and advantages amongst multiple fields. This review aims to provide insight on the elements of protein–polysaccharide complexes, how they are formed, and how they can be applied in modern material science and engineering.

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“…CS-contained coating also promoted the differentiation of osteoprogenitor cells and bone formation 30 – 32 . In recent years, researchers focused more on the applications of CS and SF mixtures in biofunctional scaffolds and coatings, which showed highly acceptable biocompatibility 33 , 34 .…”

Section: Introductionmentioning

confidence: 99%

Construction of Self-defensive Antibacterial and Osteogenic AgNPs/Gentamicin Coatings with Chitosan as Nanovalves for Controlled release

Zhou

Yan

et al. 2018

Sci Rep

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To solve the Ti implants-associated infection and poor osseointegration problems, we have constructed the AgNPs/gentamicin (Gen)-loaded silk fibroin (SF) coating with acceptable antibacterial and osteogenic aptitude. Nevertheless, due to uncontrollably sustained drug release, this bactericidal coating encountered some tricky problems, such as local high Ag concentration, short life-span and potential cytotoxicity. In this work, a chitosan (CS) barrier layer was constructed to prebuilt the SF-based film by two means, dip-coating (DCS) and spin-coating (SCS). Intriguingly, the CS barrier layer constructed by spin-coating highly improved the hydrophilic and protein-absorbed performances. As verified in the release profile, both coatings showed a prolonged and pH-dependent pattern of Ag+ with an accelerated release in acidic condition. Also, the multilayer coating with a SCS barrier layer showed an apparent bacteria-trigged antibacterial and biofilm-inhibited performances, whereas the improvements of antibacterial abilities of DCS coating were limited. The mechanisms could be explained that the pH decrease induced by the attachment and proliferation of bacteria triggered collapse of CS barrier layer, accelerating the release of bactericides. Moreover, benefitted from pH-dependent release behavior of Ag and bioactive SCS layer, functional coatings highly enhanced the initial adhesion, migration and proliferation of preosteoblast MC3T3-E1 cells, and subsequently accelerated osteoblast differentiation (alkaline phosphatase production). A relevant aspect of this work was to demonstrate the essential effect of reasonable construction of self-defensive barrier layer in achieving the balance between the high-efficiency bacterial killing and osteogenic activity, and highlighted its excellent potential in clinical applications.

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Simulation of ECM with silk and chitosan nanocomposite materials

Cited by 24 publications

References 72 publications

Water‐insoluble amorphous silk fibroin scaffolds from aqueous solutions

Water‐insoluble amorphous silk fibroin scaffolds from aqueous solutions

Protein–Polysaccharide Composite Materials: Fabrication and Applications

Construction of Self-defensive Antibacterial and Osteogenic AgNPs/Gentamicin Coatings with Chitosan as Nanovalves for Controlled release

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