Groundwater pollution is a threat to the environment and human health because it is an important source of drinking water. Groundwater is used to supply water to communities and pollution occurs when harmful substances and contaminants infiltrate into the groundwater. Through excessive use of fertilizers, agriculture is a major contributor to groundwater pollution. This study tests the impact of organic and mineral fertilization on the groundwater physiochemical parameters and explores the potential consequences of using manure as fertilizer on groundwater nitrate pollution. The experiment was carried out in Satu Mare County, Romania, where both organic (manure) and mineral fertilizers were applied to potato cultures to test their impact on 18 physiochemical parameters of groundwater quality. Basic Statistics, Nitrate Pollution Index (NPI), and Principal Components Analysis were used for emphasizing the impact of mineral and organic fertilization on groundwater quality and relationships between analyzed groundwater parameters. The results show that groundwater corresponding to the site where the higher dose of organic fertilization was applied is characterized by nitrate concentrations (64.92 mg/L) and pH values (6.3 pH units) beyond the allowed limits. Based on the calculated NPI (2.21), it falls within the significant pollution category. Two principal factors were identified as having an impact on groundwater quality: fertilizer type and administered dose, respectively.