Simultaneous Analysis and Quantification of Markers of Manjisthadi Churna Using High Performance Thin Layer Chromatography

Abstract

Manjisthadi churna has been traditionally used in the Ayurvedic system of medicine and by traditional medical practices of India to treat hyperlipidemia. A rapid, simple and accurate method with high performance thin layer chromatography has been developed to standardised Manjisthadi churna using rubiadin, sennoside and ellagic acid as markers. Methanol extract of Manjisthadi churna were used for high performance thin layer chromatography on silica gel plates. The Rf of rubiadin, sennoside-A and ellagic acid were found to 0.48, 0.23 and 0.72, respectively with densitometric scanning at 280 nm and the calibration plot were linear in the range of 100-600 ng of markers. The correlation coefficients were higher than 0.99 were indicative of good linear dependence of peaks area on concentration. The rubiadin, sennoside-A and ellagic acid contents in Manjisthadi churna were found to be 0.014, 0.038 and 0.534% w/w, respectively. This method permits reliable quantification of rubiadin, sennoside-A and ellagic acid with good resolution and separation of the same from other constitutes of the extract of Manjisthadi churna. Recovery value from 95.66-102.33% showed the reliability and reproducibility of the method. The proposed high performance thin layer chromatography method for simultaneous quantification of markers in Manjisthadi churna can be used for routine quality testing.

Mini-Review of Analytical Methods used in Quantification of Ellagic Acid

“…The ellagic acid content was 0.534% w/w in Manjisthadi churna. Recovery values from 100.75-102.33% showed the reliability and reproducibility of the method [76] (Table 2).…”

Chemistry, Biosynthesis, Physicochemical and Biological Properties of Rubiadin: A Promising Natural Anthraquinone for New Drug Discovery and Development

“…Additionally, Rubiadin is also found in a traditional medicine named Manjisthadi churna, which consists of lesser cardamom, used in the treatment of hyperlipidemia in India’s Ayurvedic system of medicine and traditional medical practices. 18 It is also found in some traditional Chinese medicines including Jia-Jian-Di-Huang-Yin-Zi decoction which is a seven-herb component consisting of Radix rehmanniae, Fructus corni, Radix morindae officinalis, Herba cistanches, Radix angelicae sinensis, Radix asparagi and Radix paeoniae alba 19 as well as another decoction named Er Xian 20 which is a six-herb component consisting of Herba epimedii, Radix morindae officinalis, Radix angelicae sinensis, Rhizoma anemarrhenae, Cortex phellodendri and Rhizoma curculiginis . Medicinal plants containing Rubiadin, Rubiadin-1-methyl ether (RBME) and Rubiadin-3-methyl ether are summarized in Table 1 .…”