Simultaneous Analysis of MLH1, MSH2, MSH6, PMS2 and KRAS in Patients with Gastric and Colon Cancer Using Stochastic Sensors

Gheorghe, Damaris-Cristina; Staden, Raluca‐Ioana Stefan‐van; Pogăcean, Florina; Pruneanu, Stela

doi:10.3390/chemosensors10100380

Cited by 7 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By comparing the results obtained in this paper with results obtained previously 25,26 using stochastic sensors, the working concentration ranges are wider, and the limits of determination are far lower favorizing the identification and quantification of MLH1, MSH2, MSH6, PMS2, and KRAS in whole blood, saliva, urine, and tumoral tissue samples, at a very early stage, just when they are released in the body. Also, comparing the screening method with the ELISA used separately for each of the biomarkers in accredited laboratories, the proposed method can be used on wider linear concentration ranges, with lower limits of determination, without processing in any way the biological sample.…”

Section: Response Characteristics Of the Miniplatformsmentioning

confidence: 58%

See 1 more Smart Citation

Miniplatforms for Screening Biological Samples for KRAS and Four Mismatch Repair Proteins as New Tools for Fast Screening for Gastric and Colon Cancers

Staden¹,

Bratei²,

Ilie-Mihai³

et al. 2023

J. Electrochem. Soc.

Self Cite

View full text Add to dashboard Cite

Two miniplatforms based on stochastic microsensors designed using Nitrogen (9.3%) and Boron (2.4%) - dopped graphene (NB-DG) modified with frutafit HD and frutafit TEX were designed and validated for the assay of MLH1, MSH2, MSH6, PMS2, and of KRAS in whole blood, urine, saliva, and tumoral tissues. The sensitivities recorded using the miniplatform based on frutafit TEX were higher (MLH1:1.07×104, MSH2: 5.31; MSH6: 1.58×103; KRAS: 1.36×10-2 s-1 μg-1 mL) than those recorded when frutafit HD was used. A lower value of the limit of determination (0.32 fg mL-1) was recorded for the frutafit HD based miniplatform when used for the assay of MLH1, while the lowest value of the limit of determination for the assay of KRAS (2.2 fg mL-1) was recorded when the frutafit TEX was used in the design of the miniplatform. The percent recoveries of MLH1, MSH2, MSH6, PMS2, and of KRAS in whole blood, urine, saliva, and tumoral tissues were higher than 99.00 with RSD (%) values lower than 0.08%.

show abstract

Section: Response Characteristics Of the Miniplatformsmentioning

confidence: 58%

“…Compared with previously reported sensors used for the assay of these biomarkers, 25,26 the proposed miniplatforms exhibited better sensitivities and lower limits of determination.…”

mentioning

confidence: 92%

Miniplatforms for Screening Biological Samples for KRAS and Four Mismatch Repair Proteins as New Tools for Fast Screening for Gastric and Colon Cancers

Staden¹,

Bratei²,

Ilie-Mihai³

et al. 2023

J. Electrochem. Soc.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Compared to the results obtained for the assay of KRAS and MLH1, MSH2, MSH6, PMS2, 25,26 using stochastic sensors, the working concentration ranges are wider, and the limits of determination are far lower, favorizing the identification and quantification of MLH1, MSH2, MSH6, PMS2, and KRAS in whole blood, saliva, urine, and tumoral tissue samples, at a very early stage of colon cancer.…”

Section: Bioanalysis Of Mmr: Mlh1 Msh2 Msh6 Pms2 and Of Kras Using Th...mentioning

confidence: 78%

Bioanalysis of MMR and KRAS – a key factor in diagnosis of colorectal cancer

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Biomarkers were bought from Sigma Aldrich. Stochastic microsensors designed for the assay of maspin, K-Ras, MLH1, and PMS2 were used for the determination of the concentrations of maspin, K-Ras, MLH1, and PMS2 [31][32][33].…”

Section: Materials and Reagentsmentioning

confidence: 99%

Differentiation between Gastric and Colorectal Adenocarcinomas Based on Maspin, MLH1, PMS2 and K-Ras Concentrations Determined Using Stochastic Sensors

Bratei,

Stefan-van Staden

2023

Gastrointestinal Disorders

Self Cite

View full text Add to dashboard Cite

Background: Gastrointestinal adenocarcinomas are a worldwide and some of the most important causes of death related to cancers. MLH1, PMS2, and K-Ras are some of the main molecules responsible for the control of cellular proliferation. They are widely used as biomarkers for the evaluation of the features of tumoral processes and the clinicopathological characteristics. They depend on the type of cells implied in the tumoral process, and it can be observed in the concentrations of them in different biological fluids. Maspin, also known as peptidase inhibitor 5 or serpin B5 is a tumor suppressor which inhibits invasion and angiogenesis and also regulates apoptosis, but it can also present oncogenic activity depending on tumor location and histology and on the subcellular maspin localization. Its correlations with gastric and colorectal carcinomas have been emphasized in a series of articles, and in this work, a method is used to quantify the concentrations of maspin in three biological fluids, allowing correlations with pathological features. Methods: Patients with their clinical and pathological features were selected from the database of the project GRAPHSENSGASTROINTES and used accordingly with the Ethics committee approval nr. 32647/2018 awarded by the County Emergency Hospital from Targu-Mures. Three kinds of samples have been analyzed (saliva, whole blood, and urine) using a stochastic method using stochastic microsensors. Results: The results obtained using stochastic sensors were correlated with the location of cancer, and there have been elaborated a series of criteria to differentiate gastric cancers from colorectal ones. Conclusions: There can be differentiation between the two types of cancers by using the concentrations of MLH1, PMS2, and K-Ras in saliva and urine samples or the levels of maspin in whole blood and urine or in whole blood, urine, and saliva. The data analysis led to a series of criteria for evaluation of the cancer location. Using only MLH1 and PMS2 concentrations in one of the two kinds of samples was only indicative and did not cover most cases. The use of the criteria only for MLH1 and PMS2 increased the probability of finding out the location, but the best results require the concentrations of K-Ras in the two kinds of samples as additional criteria.

show abstract

Simultaneous Analysis of MLH1, MSH2, MSH6, PMS2 and KRAS in Patients with Gastric and Colon Cancer Using Stochastic Sensors

Cited by 7 publications

References 26 publications

Miniplatforms for Screening Biological Samples for KRAS and Four Mismatch Repair Proteins as New Tools for Fast Screening for Gastric and Colon Cancers

Miniplatforms for Screening Biological Samples for KRAS and Four Mismatch Repair Proteins as New Tools for Fast Screening for Gastric and Colon Cancers

Bioanalysis of MMR and KRAS – a key factor in diagnosis of colorectal cancer

Differentiation between Gastric and Colorectal Adenocarcinomas Based on Maspin, MLH1, PMS2 and K-Ras Concentrations Determined Using Stochastic Sensors

Contact Info

Product

Resources

About