Simultaneous analysis of multiple bioactive constituents in Rheum tanguticum Maxim. ex Balf. by high‐performance liquid chromatography coupled to tandem mass spectrometry

Abstract

A simple, rapid method was developed using on-line high-performance liquid chromatography (HPLC)-diode array detection (DAD)/electrospray ionization mass spectrometry (ESI-MS) to simultaneously analyze multiple bioactive constituents in the extract of Rheum tanguticum Maxim. ex Balf. (Dahuang), a traditional Chinese medicine (TCM). Many bioactive constituents gave prominent [M-H]- ions in the negative ion ESI mass spectra. Among them, 41 different constituents including 16 anthraquinone derivatives, 7 phenylbutanone glucopyranosides, 4 stilbenes and 14 tannins were unambiguously identified or tentatively characterized based on their retention times, UV spectra and mass spectra in comparison with the data from standards or references. Meanwhile, some principles of fragmentation behavior for different types of constituents were proposed, which could contribute to the elucidation of these constituents in Rheum tanguticum.

Pre-column incubation followed by fast liquid chromatography analysis for rapid screening of natural methylglyoxal scavengers directly from herbal medicines: Case study of Polygonum cuspidatum

“…The elution order of the two isomers was also in agreement with previous reports. [42,43]. Meanwhile, a major ion at m/z 227 was also observed, which was identical with the [M−H] − ion of resveratrol (5), which was further supported by acidic hydrolysis of P. cuspidatum extract (details shown in Supplementary Data).…”

Aloe-Emodin Relieves High-Fat Diet Induced QT Prolongation via MiR-1 Inhibition and IK1 Up-Regulation in Rats

“…In the current study, Aloeemodin (Fig. 1A) is a monomer of phenanthrenequinones extracted from rhubarb and has a similar chemical structure with emodin [9]. Several studies have reported the protective effect of emodin against cardiac diseases.…”

Fast profiling of chemical constituents in Yiqing Capsule by ultra‐performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry

“…It was also observed that the ion at m/z 225 underwent the successive losses of a methyl group and CO molecule, resulting in ions at m/z 210 and 182. Therefore, compound 29 was reasonably identified as chrysophanol by comparing with the literature data [4,24].…”