Simultaneous analysis of piperacillin and tazobactam in rabbits: application to pharmacokinetic study

Abstract

A simple and rapid assay is developed for the simultaneous analysis of piperacillin and tazobactam in rabbit serum and tissue cage fluid (TCF). To eliminate endogenous interferences, a wavelength switch technique was applied, in which the programmable UV detector changed the monitoring wavelength from 218 to 254 nm at 10 min. After liquid-liquid extraction, sample analyses were performed on a C(18) column by gradient elution; the mobile phase consisted of acetonitrile and phosphate buffer (0.014 m, pH 2.4). Owing to the limited amount of rabbit TCF available, a cross-validation of a proxy matrix was evaluated. The relative standard deviation of the between- and within-batch precision of both compounds was less than 5.1%; the relative error of the between- and within-batch accuracy was less than 7.3%. The recoveries of both compounds in serum and TCF were larger than 80%. This assay was successfully applied to simultaneously analyze piperacillin and tazobactam in rabbit serum and TCF samples.

Simultaneous determination of five β-lactam antibiotics (cefepim, ceftazidim, cefuroxim, meropenem and piperacillin) in human plasma by high-performance liquid chromatography with ultraviolet detection

“…These limits are better or comparable to those reported previously in published HPLC methods and are sufficient to determine plasma concentrations of each of the five antibiotics in the clinical pharmacokinetic studies [7][8][9][10][11][12][13][14][15]. Insofar as in all the spiked samples, each antibiotic was easily detected, the limits of detection were at least at 0.5 g/mL.…”

“…Various methods have already been developed to analyse antibiotics in biological fluids using different sample preparation procedures: precipitation, liquid-or solid-phase extraction, generally followed by liquid chromatography coupled to UV detection [7][8][9][10][11][12][13][14][15].…”

Inoculum Effect on the Efficacies of Amoxicillin-Clavulanate, Piperacillin-Tazobactam, and Imipenem against Extended-Spectrum β-Lactamase (ESBL)-Producing and Non-ESBL-Producing Escherichia coli in an Experimental Murine Sepsis Model

“…Kocuria rhizophila ATCC 9341 was the control strain for amoxicillin and imipenem, and K. pneumoniae ATCC 29665 was the control strain for clavulanate (17,18). Serum piperacillin-tazobactam levels were quantified by high-performance liquid chromatography as previously described (19). A protein binding value of 20% for piperacillin and tazobactam was used (20).…”

Single-Dose Pharmacokinetics of Piperacillin/Tazobactam in Hispaniolan Amazon Parrots (Amazona ventralis)

“…Tazobactam has minimal antibacterial activity when used alone; it synergistically expands piperacillin's spectrum of activity against many strains of blactamase-producing bacteria. 9 Tazobactam is similar or slightly superior to clavulanic acid in its ability to protect piperacillin from hydrolysis by plasmid-and chromosome-mediated b-lactamases.…”