Simultaneous analysis of seven marker compounds from Saposhnikoviae Radix, Glehniae Radix and Peucedani Japonici Radix by HPLC/PDA

Abstract

A new combination of high performance liquid chromatography (HPLC) method coupled with photodiode array (PDA) analysis has been developed for the simultaneous quantitative determination of seven major components in Saposhnikoviae Radix (SR), Glehniae Radix (GR) and Peucedani Japonici Radix (PR), namely peucedanol 7-O-β-D-glucopyranoside (1), prim-O-glucosylcimifugin (2), cimifugin (3), 4'-O-β-D-glucosyl-5-O-methylvisamminol (4), bergapten (5), sec-O-glucosylhamaudol (6), and imperatorin (7). Clear separation of these seven components were achieved on a Phenomenex Kinetex C18 (250 × 4.6 mm, 5 μm) column by gradient elution of water (A) and methanol (B) as mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 254 nm. The method was successfully used in the analysis of SR, GR, and PR with relatively simple conditions and procedures, and the results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The results indicate that the established HPLC/PDA method is suitable for the classification of SR, GR, and PR.

Ultra‐performance convergence chromatography method for the determination of four chromones and quality control of Saposhnikovia divaricata (Turcz.) Schischk.

“…The results of LOD and LOQ concentrations indicated similar sensitivity as that observed with an HPLC method reported by Seo et al. (LODs 0.011–0.028 μg/mL, LOQs 0.036–0.094 μg/mL) . This excellent sensitivity thereby permits the detection of small quantities of the four chromones in S. divaricata .…”

“…Thus, three different quantities of the chromone standards were added to known quantitiesof S. divaricata samples, and recoveries of 99.48–102.89% were obtained with RSD values < 1.0% for the four chromones, thereby confirming the accuracy of the developed method. The results of the recovery test showed that our method had higher accuracy than any other reported method (93.92–108.92%) .…”

“…In addition, determination of the LOD, which was defined as the concentration where the ratio between the peak height of interest and the baseline noise was three (i.e., S/N ratio = 3), gave values ranging from 0.006 to 0.033 μg/mL. Furthermore, the LOQ, considered as being three times the detection limit, varied [15]. This excellent sensitivity thereby permits the detection of small quantities of the four chromones in S. divaricata.…”

“…In addition, Seo et al. recently developed an HPLC method for the simultaneous determination of seven components, including four chromones, in S. divaricata . However, these methods required long chromatographic run times of 18–50 min and employed large volumes of organic solvents due to the use of 4.6 mm i.d.…”

“…A number of HPLC methods have been developed for the quantitative determination of chromones in S. divaricata, with Li et al [14] and Gui et al [13] reporting RP-HPLC methods for the simultaneous analysis of the four chromones of S. divaricata. In addition, Seo et al [15] recently developed an HPLC method for the simultaneous determination of seven components, including four chromones, in S. divaricata. However, these methods required long chromatographic run times of 18-50 min and employed large volumes of organic solvents due to the use of 4.6 mm i.d.…”