Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Abstract

Recebido em 14/10/12; aceito em 22/2/13; publicado na web em 24/5/13In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R 2 > 0.999) over the concentration range of 10-500 ng mL −1. The statistical evaluation of the method was carried out by performing the intra-and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.

Ethnomedicinal Information and High-Performance Liquid Chromatography Analysis of Water Soluble Vitamins (C, B1, B3, B6, folic acid) and Fat Soluble Vitamins (A, D3, E) of Three Consumable Parts of Musa paradisiaca: Cultivated in Tripura, India

“…Ultraviolet (UV) absorbance was recorded at 270 nm at room temperature [11,12]. To determine the B group vitamins, the quality assurance (QA) and quality control (QC) for the HPLC were regulated by the method of Kucukkolbasi et al, (2013) with some modifications [13].…”

“…Twenty microliters of extracted oil was directly injected into the HPLC column in triplicate [11,14]. To determine the B group vitamins, the QA and QC for the HPLC were regulated by the method of Kucukkolbasi et al, (2013) with some modifications [13].…”

Novel HPLC-UV Method for Simultaneous Determination of Fat-soluble Vitamins and Coenzyme Q10 in Medicines and Supplements

“…The obtained run time is considerably shorter in comparison to other similar published HPLC-UV methods. [4][5][6]9,12 …”

“…Due to the large number of commercially available vitamin preparations and their widespread use, the quality control of these preparations is extremely important, especially because of the risk of toxicity from excessive intake of vitamins A and D. 3 HPLC methods, coupled with different detection techniques: UV, [4][5][6][7][8][9][10][11][12] FLD, 6 ECD, 13 and MS 5,14 offer the best approach to accurate content determination of the main FSVs in foods, pharmaceuticals and nutritional supplements. The published HPLC-UV methods for quality control of preparations containing FSVs are quite limited in terms of separation and simultaneous determination of FSVs, 4,5,7,8,11 often have run time longer than 15 min [4][5][6]9,12 and generally require complicated and time-consuming sample preparation. 11,12,15,16 Further on, to our knowledge none of the published HPLC methods offer simultaneous determination of FSVs and also β-carotene and coenzyme Q10, which are often found along in medicines and nutritional supplements.…”

A novel RP-HPLC method for simultaneous determination of vitamins B1, B2, B3, B6 and C in oral powder for veterinary consumption

“…From the aforesaid methods, RP-HPLC is extensively used instead of other methods because of its higher sensitivity. The existing RP-HPLC methods are for determination of multivitamin in human blood and plasma [6], capsules [7] and tablet dosage forms [8][9][10][11][12][13]. The main advantage of our study is to have a simple, specific, precise, accurate and linear reverse phase gradient HPLC method for qualitative, quantitative and simultaneous determination of vitamin B1, vitamin B2, vitamin B3, vitamin B6 and vitamin C in solid oral powder for veterinary consumption that was not reported before for these five vitamins at the same time.…”