Simultaneous characterization and quantitation of 11 coumarins in Radix Angelicae Dahuricae by high performance liquid chromatography with electrospray tandem mass spectrometry

Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

“…More recently, it was reported that Angelicae dahuricae radix suppressed the development of atopic dermatitis-like skin lesions and could be used for the treatment of hypertension and asthma (3,7,8). A number of analytical methods have been reported for the determination of several coumarins in Angelicae dahuricae radix, including capillary electrochromatography, high-performance liquid chromatography, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry and high-speed countercurrent chromatography (7,(9)(10)(11)(12). It appears that the crude extract and essential oils of Angelicae dahuricae radix may include multiple potentially active chemical compounds.…”

Analytical Methods for Isolation, Separation and Identification of Selected Furanocoumarins in Plant Material

“…The furanocoumarin determination from air-dried plant material was also performed using 75% methanol in an ultrasonic ice-water bath , with 100% methanol (Cardoso et al, 2000;Ojala, 2000), 70% methanol (Chen & Sheu, 1995), with hot (70˚C) pure methanol on a water bath (Bartnik & Głowniak, 2007), with pure ethanol in heated reflux (Yu et al, 2002), with 95% ethanol at 80˚C Zheng et al, 2010), with acetone at room temperature (Taniguchi et al, 2011), with ether at 40˚C (Liu et al, 2004a), with dichloromethane at room temperature (Um et al, 2010), with petroleum ether at room temperature (Tosun et al, 2008), with chloroform in a sonic bath (Cardoso et al, 2000).…”

“…Frequently, residuals after methanol/ethanol extractions were suspended in water and portioned a few times with chloroform or petroleum ether Zheng et al, 2010).…”

“…A paper by Zheng et al (2010) reports the quantitation of eleven coumarins including furocoumarins in Radix Angelicae dahuricae. By using this HPLC-ESI-MS/MS method, all coumarins were separated and determined within 10 min.…”

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

“…All five compounds showed good linearity (R 2 > 0.9993) over the concentration ranges of 2-150 ng/ml. In another study, 11 coumarins (scopoletin, xanthotoxol, xanthotoxin, psoralen, isoimpinellin, bergapten, oxypeucedanin, imperatorin, cnidilin, osthole and isoimperatorin) in A. dahuricae roots were determined using Q-Trap mass spectrometer within 10 min [197].…”