Simultaneous colonization by Escherichia coli and Klebsiella pneumoniae harboring mcr-1 in Brazil

Neto, Lauro Vieira Perdigão; Corscadden, Louise; Martins, Roberta Cristina Ruedas; Nagano, Debora Satie; Cunha, Marcos Paulo Vieira; Neves, Patrícia; Franco, Lucas Augusto Moysés; Moura, Luísa; Rizek, Camila; Guimarães, Thaís; Boszczowski, Ícaro; Rossi, Flávia; Levin, Anna S.; Stabler, Richard A.; Costa, Sílvia Figueiredo

doi:10.1007/s15010-019-01309-2

Cited by 14 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ST744 isolates belonged to phylogroup A and E. coli in this phylogroup are not as virulent as those belonging to phylogroups B2 and D2 ( 80 ). Nonetheless, ST744 has been sporadically associated as a disease-causing agent carrying resistance to critical antimicrobials such as colistin and carbapenem from human patients worldwide ( 81 – 83 ). The global distribution of fluoroquinolone-resistant ST744 clones should be worrisome, as fluoroquinolones are critical antimicrobials for treating systemic infections in humans ( 84 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States

Hayer

Lim

Hong

et al. 2020

mSphere

View full text Add to dashboard Cite

Fluoroquinolones and cephalosporins are critically important antimicrobial classes for both human and veterinary medicine. We previously found a drastic increase in enrofloxacin resistance in clinical Escherichia coli isolates collected from diseased pigs from the United States over 10 years (2006 to 2016). However, the genetic determinants responsible for this increase have yet to be determined. The aim of the present study was to identify and characterize the genetic basis of resistance against fluoroquinolones (enrofloxacin) and extended-spectrum cephalosporins (ceftiofur) in swine E. coli isolates using whole-genome sequencing (WGS). blaCMY-2 (carried by IncA/C2, IncI1, and IncI2 plasmids), blaCTX-M (carried by IncF, IncHI2, and IncN plasmids), and blaSHV-12 (carried by IncHI2 plasmids) genes were present in 87 (82.1%), 19 (17.9%), and 3 (2.83%) of the 106 ceftiofur-resistant isolates, respectively. Of the 110 enrofloxacin-resistant isolates, 90 (81.8%) had chromosomal mutations in gyrA, gyrB, parA, and parC genes. Plasmid-mediated quinolone resistance genes [qnrB77, qnrB2, qnrS1, qnrS2, and aac-(6)-lb′-cr] borne on ColE, IncQ2, IncN, IncF, and IncHI2 plasmids were present in 24 (21.8%) of the enrofloxacin-resistant isolates. Virulent IncF plasmids present in swine E. coli isolates were highly similar to epidemic plasmids identified globally. High-risk E. coli clones, such as ST744, ST457, ST131, ST69, ST10, ST73, ST410, ST12, ST127, ST167, ST58, ST88, ST617, ST23, etc., were also found in the U.S. swine population. Additionally, the colistin resistance gene (mcr-9) was present in several isolates. This study adds valuable information regarding resistance to critical antimicrobials with implications for both animal and human health. IMPORTANCE Understanding the genetic mechanisms conferring resistance is critical to design informed control and preventive measures, particularly when involving critically important antimicrobial classes such as extended-spectrum cephalosporins and fluoroquinolones. The genetic determinants of extended-spectrum cephalosporin and fluoroquinolone resistance were highly diverse, with multiple plasmids, insertion sequences, and genes playing key roles in mediating resistance in swine Escherichia coli. Plasmids assembled in this study are known to be disseminated globally in both human and animal populations and environmental samples, and E. coli in pigs might be part of a global reservoir of key antimicrobial resistance (AMR) elements. Virulent plasmids found in this study have been shown to confer fitness advantages to pathogenic E. coli strains. The presence of international, high-risk zoonotic clones provides worrisome evidence that resistance in swine isolates may have indirect public health implications, and the swine population as a reservoir for these high-risk clones should be continuously monitored.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States

Hayer

Lim

Hong

et al. 2020

mSphere

View full text Add to dashboard Cite

show abstract

“…The mcr-1 gene can also be integrated into the chromosome of some strains (17). However, in Latin America, only four plasmids have been described so far: IncX4 (36), IncP (22), IncI2 (31), and IncHI2 (37), of which the IncX4 plasmid is the most frequent in Brazil (38,39) (Table 1). There is a clear association between the IncX4 plasmids and the insertion sequences associated with the dissemination of the mcr-1 gene (40).…”

Section: Genetic Context and Dissemination Of Mcr-1 Genementioning

confidence: 99%

mcr-1 Gene in Latin America: How Is It Disseminated Among Humans, Animals, and the Environment?

Lentz

Dalmolin

Barth

et al. 2021

Front. Public Health

View full text Add to dashboard Cite

“…MDR E. coli ST744 isolates have been found in multiple animal species, environments, and diseased humans worldwide. ST744, mcr-1 positive, isolates have been obtained from human bloodstream infection in Denmark [90], human fecal samples in China and Brazil [91,92], UTI in Portugal and Brazil [93,94], and sputum/body fluid in China [95]. E. coli ST744 strains have also been reported in mcr-1-positive isolates from poultry in Romania and Lebanon [96,97], weaning pigs in Japan [98], while mcr-3-producing E. coli ST744 isolate was reported in veal calves in France [99].…”

Section: Discussionmentioning

confidence: 99%

First Report and Characterization of the mcr-1 Positive Multidrug-Resistant Escherichia coli Strain Isolated from Pigs in Croatia

Kompes,

Duvnjak,

Reil

et al. 2023

Microorganisms

View full text Add to dashboard Cite

The emergence and rapid spread of the plasmid-mediated colistin-resistant mcr-1 gene introduced a serious threat to public health. In 2021, a multi-drug resistant, mcr-1 positive Escherichia coli EC1945 strain, was isolated from pig caecal content in Croatia. Antimicrobial susceptibility testing and whole genome sequencing were performed. Bioinformatics tools were used to determine the presence of resistance genes, plasmid Inc groups, serotype, sequence type, virulence factors, and plasmid reconstruction. The isolated strain showed phenotypic and genotypic resistance to nine antimicrobial classes. It was resistant to colistin, gentamicin, ampicillin, cefepime, cefotaxime, ceftazidime, sulfamethoxazole, chloramphenicol, nalidixic acid, and ciprofloxacin. Antimicrobial resistance genes included mcr-1, blaTEM-1B, blaCTX-M-1, aac(3)-IId, aph(3’)-Ia, aadA5, sul2, catA1, gyrA (S83L, D87N), and parC (A56T, S80I). The mcr-1 gene was located within the conjugative IncX4 plasmid. IncI1, IncFIB, and IncFII plasmids were also detected. The isolate also harbored 14 virulence genes and was classified as ST744 and O101:H10. ST744 is a member of the ST10 group which includes commensal, extraintestinal pathogenic E. coli isolates that play a crucial role as a reservoir of genes. Further efforts are needed to identify mcr-1-carrying E. coli isolates in Croatia, especially in food-producing animals to identify such gene reservoirs.

show abstract

Simultaneous colonization by Escherichia coli and Klebsiella pneumoniae harboring mcr-1 in Brazil

Cited by 14 publications

References 12 publications

Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States

Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States

mcr-1 Gene in Latin America: How Is It Disseminated Among Humans, Animals, and the Environment?

First Report and Characterization of the mcr-1 Positive Multidrug-Resistant Escherichia coli Strain Isolated from Pigs in Croatia

Contact Info

Product

Resources

About