Simultaneous Detection and Prevalence of Allergens in Anisakis Species Isolated from Marine Fishes

Abstract

This study was conducted to develop a multiplex reverse transcription (RT) PCR for the detection of Anisakis allergens and to investigate the relationship between allergen profiles and anisakid larvae isolated from Scomber japonicus, Trichiurus lepturus, and Conger myriaster in Korea. The species of Anisakis was determined using Anisakis pegreffii-specific PCR and restriction fragment length polymorphism analysis. The prevalence and profiles of five Ani s allergens were examined by multiplex RTPCR. A. pegreffii and Anisakis typica accounted for 97.1 and 2.9%, respectively, of the 140 larvae examined. In A. pegreffii, allergen prevalence was 41.2% for Ani s 1, 72.1% for Ani s 2, 69.9% for Ani s 3, 86.7% for Ani s 4, and 93.4% for Ani s 5. Most A. pegreffii larvae had multiple allergen profiles, and 80.7% of A. pegreffii carried both Ani s 4 and Ani s 5, which are heat-resistant allergens. Fifty-two to 65% of A. pegreffii isolated from S. japonicus and C. myriaster carried all five Ani s allergens.

First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water

“…A unique distribution pattern has been known for A. typica which has been reported in warmer temperate and tropical waters (Mattiucci and Nascetti, 2006). In Asian countries, A. typica larvae have been reported in Japan, Korea, China, Taiwan, Indonesia, and Thailand (Lee et al, 2016;Palm et al, 2008Palm et al, , 2017Sonko et al, 2019;Tunya et al, 2020;Umehara et al, 2010; JOURNAL OF NEMATOLOGY Zhu et al, 2007). In the present study, we firstly identified A. typica larvae in Vietnamese water.…”

“…Then, the larvae were observed and measured under a light microscope (ECLIPSE H600 L Nikon). For scanning electron microscopy, Anisakis larvae were prepared according to Madden and Tromba (1976) and Morsy et al (2017). Larvae were identified according to the reported references (Berland, 1961;Chen and Shih, 2015;Mattiucci et al, 2009;Sonko et al, 2019;Tunya et al, 2020).…”

Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System

“…A. pegreffii third-stage larvae (L3) were manually harvested from the abdominal cavity. The genotypes of A. pegreffii and anisakid larvae were confirmed with polymerase chain reaction (PCR) using the protocol described by Lee et al The primers used were ASF1 5′-CAG CTT AAG GCA GAG TC-3′ and AS2 5′-TAT CAT TTT TGA TCA CAT AGA C-3′ [ 38 ].…”