Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor

Wang, Xiaokun; Choi, Nak Sam; Cheng, Ziyi; Ko, Juhui; Chen, Lingxin; Choo, Jaebum

doi:10.1021/acs.analchem.6b03536

Cited by 226 publications

(113 citation statements)

References 32 publications

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“…Jaebum Choo's team presented a few modifications of a sensor where malachite green isothiocyanate was adsorbed on gold nanoparticles with detection DNA and then hybridized with the target DNA and capture DNA (modified with biotin and streptavidin). Two of their works have applied to lateral flow strips, where this structure has been used as an alternative to the typical oligonucleotide-labelled AuNPs [90,91]. Their sensor was able to detect two DNA markers simultaneously.…”

Section: Sandwich Sensors With Rr Located On Nanoparticlesmentioning

confidence: 99%

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

et al. 2019

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A sensitive and accurate identification of specific DNA fragments (usually containing a mutation) can influence clinical decisions. Standard methods routinely used for this type of detection are PCR (Polymerase Chain Reaction, and its modifications), and, less commonly, NGS (Next Generation Sequencing). However, these methods are quite complicated, requiring time-consuming, multi-stage sample preparation, and specially trained staff. Usually, it takes weeks for patients to obtain their results. Therefore, different DNA sensors are being intensively developed by many groups. One technique often used to obtain an analytical signal from DNA sensors is Raman spectroscopy. Its modification, surface-enhanced Raman spectroscopy (SERS), is especially useful for practical analytical applications due to its extra low limit of detection. SERS takes advantage of the strong increase in the efficiency of Raman signal generation caused by a local electric field enhancement near plasmonic (typically gold and silver) nanostructures. In this condensed review, we describe the most important types of SERS-based nanosensors for genetic studies and comment on their potential for becoming diagnostic tools.

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Section: Sandwich Sensors With Rr Located On Nanoparticlesmentioning

confidence: 99%

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

et al. 2019

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“…Colorimetric biosensor is a simple and intuitive method for biomolecules assay. Quantitative detection of biomarkers can be achieved by distincting the generated color signal with naked eyes or a simple absorption spectra device . A commonly used type of signal label, gold (Au) nanoparticles with inherent red color, has been widely applied to detect various biomarkers .…”

Section: Biosensing Application Of Nano‐bpmentioning

confidence: 99%

Recent Advances on Black Phosphorus for Biomedicine and Biosensing

Xia

Guo

2019

Adv Funct Materials

200

115

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Black phosphorus nanostructures (nano-BPs) include BP nanosheets, BP quantum dots, and BP nanoparticles. Since first being discovered in 2014, nano-BP has become one of the most popular nanomaterials. Nano-BP has many unique properties, such as excellent surface activity, tunable bandgap, high carrier mobility, moderate on/off ratio, excellent biocompatibility, good biodegradation, etc., all of which make nano-BP particularly attractive in biomedicine and biosensing. This review article comprehensively summarizes recent advances in synthesis, functionalization, biomedicine, and biosensing applications of nano-BP. Different methods are first introduced, such as mechanical cleavage, liquid-phase ultrasonic exfoliation, electrochemical exfoliation, solvothermal treatment, and acoustic-microfluidic stripping, for making the nano-BP. Then two strategies are emphasized to enhance ambient stability of nano-BP, namely physical encapsulation and chemical modification. Next, how to develop nano-BP as advanced imaging agents, nanocarriers, and nanomedicine for bioimaging (fluorescence imaging, thermal imaging, and photoacoustic imaging) and disease treatment (phototherapy and photo/chemical/immune synergistic therapy) is demonstrated. The biosensing applications on nano-BP is introduced, including electrochemical biosensor, fluorescence biosensor, chemiluminescence biosensor, electrogenerated chemiluminescence biosensor, and colorimetric biosensor. Finally, the current challenges and future perspectives on nano-BP in bioapplications are discussed. Nano-BP SynthesisLayered nanomaterials are usually exfoliated from bulk crystals. [35] Compared with bulk materials, monolayer or fewlayer nanomaterials possess the enhanced surface area, excellent physiochemical activity and optoelectronic property. The exfoliation from bulk crystal to monolayer or few-layer nanosheets has been widely applied for preparing almost all the 2D nanomaterials, graphene, MoS 2 , MXene nanosheets, etc. [36] Bulk BP, similar to graphite, is the stacking of layered BP nanosheets via the weak van der Waals force. The destruction of the external driving forces to the weak van der Waals interaction between the stack layers greatly contributes to the exfoliation of nano-BP from the bulk counterparts. Mechanical exfoliation and liquid phase ultrasonic exfoliation are the two most popular approaches, and some other methods were also involved, electrochemical exfoliation and solvothermal treatment, for instance. Table 1 summarizes the advantages and limitations of different exfoliation methods. Mechanical ExfoliationMechanical exfoliation, also called scotch-tape delamination, mainly utilizes the larger binding energy between the substrate and layered materials than van der Waals forces in Figure 2. a) Schematic illustration of meta-assisted mechanical exfoliation for FLBP. b) The left is FLBPs exfoliated using the normal scotch-tape microcleavage, and the right is FLBP exfoliated using Au-assisted method, and c) the total area of FLBP on 10 different samples. ...

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“…Wang et al developed a new class SERS-based lateral flow assay (LFA) to simultaneously detect dual DNA markers as illustrated in Fig. 4A [106]. The LFA strip in this sensor consisted of two test lines and one control line.…”

Section: Nucleic Acidsmentioning

confidence: 99%

“…The application of this platform were further explored in the clinic samples [104]. [106], [108] and [109] …”

Section: Nucleic Acidsmentioning

confidence: 99%

SERS Nanotags and Their Applications in Biosensing and Bioimaging

et al. 2018

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Owing to the unique advantages of surface enhanced Raman scattering (SERS) in high sensitivity, specificity, multiplexing capability and photostability, it has been widely used in many applications, among which SERS biosensing and bioimaging are the focus in recent years. The successful applications of SERS for non-invasive biomarker detection and bioimaging under in vitro, in vivo and ex vivo conditions, offer significant clinical information to improve diagnostic and prognostic outcomes. This review provides recent developments and applications of SERS, in particular SERS nanotags in biosensing and bioimaging, describing case studies in which different types of biomarkers have been investigated, as well as outlining future challenges that need to be addressed before SERS sees both pathological and clinical use.

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Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor

Cited by 226 publications

References 32 publications

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

Recent Advances on Black Phosphorus for Biomedicine and Biosensing

SERS Nanotags and Their Applications in Biosensing and Bioimaging

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