Simultaneous detection of membrane protein and mRNA at single extracellular vesicle level by droplet microfluidics for cancer diagnosis

Lin, Huixian; Li, Bo; Guo, Jingyun; Mai, Xueying; Yu, Haiyang; Pan, Weilun; Wu, Bodeng; Liu, Wei; Zhong, Mingzhen; Liao, Tong; Zhang, Ye; Situ, Bo; Yan, Xiaohui; Liu, Yifan; Liu, Chunchen; Zheng, Lei

doi:10.1016/j.jare.2024.08.026

Journal of Advanced Research

2024

DOI: 10.1016/j.jare.2024.08.026

|View full text |Cite

Simultaneous detection of membrane protein and mRNA at single extracellular vesicle level by droplet microfluidics for cancer diagnosis

Huixian Lin,

Bo Li,

Jingyun Guo

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

2025

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Surface modification of cellulose acetate membrane for fabrication of microfluidic platforms for express extracellular vesicle-based liquid biopsy

Moiseeva,

Kozhevnikova,

Yashchenok

et al. 2025

Microchemical Journal

View full text Add to dashboard Cite

Surface modification of cellulose acetate membrane for fabrication of microfluidic platforms for express extracellular vesicle-based liquid biopsy

Moiseeva,

Kozhevnikova,

Yashchenok

et al. 2025

Microchemical Journal

View full text Add to dashboard Cite

A Novel Microfluidic Platform for Personalized Anticancer Drug Screening Through Image Analysis

Lipreri,

Totaro,

Boos

et al. 2024

Micromachines

View full text Add to dashboard Cite

The advancement of personalized treatments in oncology has garnered increasing attention, particularly for rare and aggressive cancer with low survival rates like the bone tumors osteosarcoma and chondrosarcoma. This study introduces a novel PDMS–agarose microfluidic device tailored for generating patient-derived tumor spheroids and serving as a reliable tool for personalized drug screening. Using this platform in tandem with a custom imaging index, we evaluated the impact of the anticancer agent doxorubicin on spheroids from both tumor types. The device produces 20 spheroids, each around 300 µm in diameter, within a 24 h timeframe, facilitating assessments of characteristics and reproducibility. Following spheroid generation, we measured patient-derived spheroid diameters in bright-field images, calcein AM-positive areas/volume, and the binary fraction area, a metric analyzing fluorescence intensity. By employing a specially developed equation that combines viability signal extension and intensity, we observed a substantial decrease in spheroid viability of around 75% for both sarcomas at the highest dosage (10 µM). Osteosarcoma spheroids exhibited greater sensitivity to doxorubicin than chondrosarcoma spheroids within 48 h. This approach provides a reliable in vitro model for aggressive sarcomas, representing a personalized approach for drug screening that could lead to more effective cancer treatments tailored to individual patients, despite some implementation challenges.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Simultaneous detection of membrane protein and mRNA at single extracellular vesicle level by droplet microfluidics for cancer diagnosis

Cited by 2 publications

References 53 publications

Surface modification of cellulose acetate membrane for fabrication of microfluidic platforms for express extracellular vesicle-based liquid biopsy

Surface modification of cellulose acetate membrane for fabrication of microfluidic platforms for express extracellular vesicle-based liquid biopsy

A Novel Microfluidic Platform for Personalized Anticancer Drug Screening Through Image Analysis

Contact Info

Product

Resources

About