Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR

Noh, Ji Yeong; Yoon, Sun‐Woo; Kim, Doo‐Jin; Lee, Moo‐Seung; Kim, Ji Hyung; Na, Woonsung; Song, Daesub; Jeong, Dae Gwin; Kim, Hye Kwon

doi:10.1007/s00705-017-3281-9

Cited by 86 publications

(74 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Real-time reverse transcriptase-PCR (RT-PCR) detection is currently favored for the detection of coronavirus because of its advantages as a specific, and simple quantitative assay. Moreover, realtime RT-PCR is more sensitive than the conventional RT-PCR assay, which help much for the diagnosis in early infection [10,11]. Therefore, the real-time RT-PCR assay still is a predominant method to be applied for the detection of all kinds of coronaviruses [12,13], including SARS-CoV-2 [14].…”

Section: Pcr-based Methodsmentioning

confidence: 99%

Recent advances and perspectives of nucleic acid detection for coronavirus

Shen

Zhou

et al. 2020

Journal of Pharmaceutical Analysis

486

423

View full text Add to dashboard Cite

Keywords:Coronavirus Nucleic acid detection PCR-Based methods Isothermal nucleic acid amplification-based methods Microarray-based methods a b s t r a c tThe recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) in Chinais posing a great threat to global public health. Therefore, rapid and a accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving people's lives and preventing epidemics. It is important to establish a quick standard diagnostic test for the detection of the infectious disease (COVID-19) to prevent subsequent secondary spread. Polymerase chain reaction (PCR) is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity. Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler operation. A variety of improved or new approaches also have been developed. This review summarizes the currently available detection methods for coronavirus nucleic acid. It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coronavirus infection.

show abstract

Section: Pcr-based Methodsmentioning

confidence: 99%

Recent advances and perspectives of nucleic acid detection for coronavirus

Shen

Zhou

et al. 2020

Journal of Pharmaceutical Analysis

486

423

View full text Add to dashboard Cite

show abstract

“…A duplex RT‐PCR method has been developed based on primers and probes targeting the conserved spike S2 gene of SARS‐CoV and MERS‐CoV. By using pUC57SARS‐pS2 as a template for SARS‐CoV and pGEM‐MERSS2 as a template for MERS‐CoV, respectively, adequate detection limits of 50 to 100 copies/mL were achieved 28 . The singleplex RT‐iiPCR assays are designed to detect MERS‐CoV envelope gene (upE) and open reading frame 1a (ORF1a) genes separately.…”

Section: Coronavirus and Its Diagnostic Approachesmentioning

confidence: 99%

Recent advances in the detection of respiratory virus infection in humans

Zhang

Wang

Deng

et al. 2020

Journal of Medical Virology

445

363

View full text Add to dashboard Cite

Respiratory tract viral infection caused by viruses or bacteria isone of the most common diseases in human worldwide, while those caused by emerging viruses, such as the novel coronavirus, 2019-nCoV that caused the pneumonia outbreak in Wuhan, China most recently, have posed great threats to global public health. Identification of the causative viral pathogens of respiratory tract viral infections is important to select an appropriate treatment, save people's lives, stop the epidemics, and avoid unnecessary use of antibiotics. Conventional diagnostic tests, such as the assays for rapid detection of antiviral antibodies or viral antigens, are widely used in many clinical laboratories. With the development of modern technologies, new diagnostic strategies, including multiplex nucleic acid amplification and microarray-based assays, are emerging. This review summarizes currently available and novel emerging diagnostic methods for the detection of common respiratory viruses, such as influenza virus, human respiratory syncytial virus, coronavirus, human adenovirus, and human rhinovirus. Multiplex assays for simultaneous detection of multiple respiratory viruses are also described. It is anticipated that such data will assist researchers and clinicians to develop appropriate diagnostic strategies for timely and effective detection of respiratory virus infections. K E Y W O R D S adenovirus, coronavirus, diagnostic methods, influenza virus, respiratory syncytial virus, respiratory viral infection, rhinovirus

show abstract

“…Reverse transcription polymerase chain reaction (RT-PCR) is a technology combining RNA reverse transcription (RT) with polymerase chain amplification (PCR) of cDNA. A duplex RT-PCR assay can be used to detect SARS-CoV and MERS-CoV using pUC57SARS-pS2 and pGEM-MERSS2 as templates, respectively [12]. Also, samples collected from the upper respiratory tract (oropharyngeal and nasopharyngeal) and lower respiratory tract (endotracheal aspirate, expectorated sputum, or bronchoalveolar lavage) of suspected 2019-nCoV patients can be diagnosed by RT-PCR [8].…”

mentioning

confidence: 99%