Simultaneous Detection of Six Different Types of Pesticides by an Immunosensor Based on Surface Plasmon Resonance

Míyake, Shiro; Hirakawa, Yuki; Yamasaki, Tomomi; Watanabe, Eiji; Harada, Ayako; Iwasa, Seiji; Narita, Hiroshi

doi:10.2116/analsci.19p333

Cited by 15 publications

(6 citation statements)

References 19 publications

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“…Pseudomonas aeruginosa, a foodborne pathogen, was detected using SEF technique where (R)-4-(anthracen-9-yl)-6-(naphthalen-1-yl)-1,6-dihydropyrimidine-2-amine (ANDPA) as fluorescence probe and glucose stabilized silver nanoparticles (Glu-AgNPs) in various food samples including milk, orange juice and sugarcane (Ellairaja, Krithiga, Ponmariappan, & Vasantha, 2017). Due to their specificity, immunosensors could be used in the agrifood sector for monitoring different types of molecules from pesticides (Miyake et al, 2019), bacterial and fungal toxins (Commission, 2006, p. 364;Mayer, Färber, & Geisen, 2003;Organization & Cancer, 1993;Park, Kim, Kim, & Ko, 2014;Patel, 2004;Squire, 1981), drug traces (Elliott et al, 1998;Lu et al, 2012;Smith, Ehrenfried, Dalidowicz, & Turberg, 2002), and allergens (Ashley et al, 2017;Pollet et al, 2011;Rebe Raz, Liu, Norde, & Bremer, 2010;Tomassetti et al, 2013;Yman, Eriksson, Johansson, & Hellens, 2006). SPR biosensors can detect a specific biomarker of a pathogen even in a fmol range (Bhunia, 2008;Rasooly & Herold, 2006;Vidic et al, 2013).…”

Section: Plasmonic Immunosensorsmentioning

confidence: 99%

Plasmonic biosensors for food control

Balbinot

Srivastav

Vidić

et al. 2021

Trends in Food Science & Technology

123

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Background: Food safety is becoming increasingly important because food industry must provide quality products to minimize the health risks. Traditional methods to assure food safety, such as plate count and polymerase chain reaction are accurate and robust but can hardly satisfy the needs of the food industry because they are costly and time consuming. Therefore, optical biosensors that can analyze food in a low-cost, facile, fast, sensitive, and selective manner started to emerge. Scope and approach: This review presents plasmonic biosensors including surface plasmon resonance (SPR), localized SPR (LSPR), fiber optic SPR (FO-SPR), surface enhanced Raman scattering (SERS), surface-enhanced fluorescence (SEF), and total internal reflection (TIR) based sensors and their applications in food pathogens monitoring. Moreover, the strengths and weaknesses of plasmonic biosensors implementation in food control are showcased. Key findings and conclusions: Plasmonic biosensors could simplify procedure and radically reduce time, price and consummation of reactants, compared to traditional microbiological methods. Optical biosensors, in particular SPR, have been developed for detection of different foodborne pathogens. In parallel, analytical improvements have been achieved by coupling different techniques (fiber optics, Raman, fluorescence, luminescence) to plasmonic sensors in order to reduce the limits of detection and to improve sensitivity. The future improvements include the miniaturization of instruments to handheld devices and simplification of analysis to enable direct target detection in food matrices. Plasmonic technology can certainly have long lasting impact because the need for a simple and rapid food assay is pressing and guarantees the future development in this field.

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Section: Plasmonic Immunosensorsmentioning

confidence: 99%

Plasmonic biosensors for food control

Balbinot

Srivastav

Vidić

et al. 2021

Trends in Food Science & Technology

123

View full text Add to dashboard Cite

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“…15,16 The main optical immunosensors stand out the colorimetric, [17][18][19] such as paper-based tests, and the SPR-based ones. [20][21][22] One of the most critical aspects of the immunosensor construction is the immobilization methods employed for capture antibody. The self-assembled monolayer (SAM) is quite well employed, promoting the molecule's immobilization by forming organic films with high stability.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of PAMAM Dendrimers (G3, G4, and G5) in the Construction of a SPR-based Immunosensor for Cardiac Troponin T

2021

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A SPR-based immunosensor was developed using a SAM of an alkanethiol associated with dendrimers PAMAM(G4) to enhance the sensitivity for troponin T detection in blood samples. The feasibility of using three-dimensional platforms based on dendrimers for the development of immunosensors was demonstrated by evaluating three different generations of these dendrimers (G3, G4, and G5) to detect troponin T.The results showed the efficiency of these 3D platforms in anchoring biomolecules, amplifying the detection of troponin T. The sandwich assay showed good performance for troponin T detection, using secondary monoclonal antibodies, in the concentration range of 5-300 ng mL −1 (0.14 -8.67 nmol L −1 ), R 2 =0.991, with a LOD of 3.6 ng mL −1 .The sandwich assay's applicability was demonstrated by evaluating a secondary polyclonal antibody's performance in the concentration range of 3-30 ng mL −1 , R 2 =0.998, with a LOD of 0.98 ng mL −1 . The immunosensor was applied to determine troponin T in blood plasma samples from healthy patients, with an average recovery of 88% to 104%. The performance of the SPR-based immunosensor indicates reliable results, contributing to the rapid diagnosis of heart attack, with reduced costs.

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“…1,2 To date, the SPR biosensor has been widely applied in the fields of clinical diagnosis, environmental monitoring, and food safety. 3,4 However, the SPR biosensor exhibits poor sensitivity to small changes in refractive index, such as binding of small molecules to the metal surface. 1,5 Therefore, the binding protein on the immunosensor chip has become an alternative approach to increase the quality of binding substance and enhance the sensitivity of the SPR sensor.…”

Section: Introductionmentioning

confidence: 99%

Strategy of Dimercaptothiol as Self-assembled Monolayers Enhance the Sensitivity of SPR Immunosensor for Detection of Salbutamol

Wang

et al. 2021

ANAL. SCI.

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The formation of self-assembled monolayers (SAMs) is a normal method for the immobilization of biorecognition elements immobilized on SPR sensors. With this method, mercaptopropionic acid (MPA) with carboxylic and thiol group is the most commonly used. Dimercaptosuccinic acid (DMSA) having two carboxylics and two thiol groups is a classical antidote for heavy metal ions. In this paper, DMSA was first used to form SAMs to connect the antigen on the chip of a surface plasmon resonance (SPR) immunosensor for detection of salbutamol (SAL), and the results were compared with a traditional (MPA)-SPR sensor. Dihydrolipoic acid(DHLA)-SPR showed that the recognition efficiency of antigen and antibody of DMSA-SPR immunosensor was 170.1% at room temperature with the linear range of 5 -150 ng/mL. The recovery rate of this sensor applied to SAL detection in pork reached 94.9 -108.0% and the limit of quantification (LOQ) was 5 ng/mL. The results were in good correlation with the analysis results of ultra-high phase liquid chromatographytandem mass spectrometry (UPLC-MS/MS) analysis. This novel DMSA-SPR immunosensor provides insight into a new idea and method for improving the sensitivity of SPR immunosensors and can be widely used in the detection of other small molecules.

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Simultaneous Detection of Six Different Types of Pesticides by an Immunosensor Based on Surface Plasmon Resonance

Cited by 15 publications

References 19 publications

Plasmonic biosensors for food control

Plasmonic biosensors for food control

Evaluation of PAMAM Dendrimers (G3, G4, and G5) in the Construction of a SPR-based Immunosensor for Cardiac Troponin T

Strategy of Dimercaptothiol as Self-assembled Monolayers Enhance the Sensitivity of SPR Immunosensor for Detection of Salbutamol

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