Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array

King, Eva-Maria; Vailes, Lisa D.; Tsay, A.; Satinover, S.M.; Chapman, Martin D.

doi:10.1016/j.jaci.2007.06.043

Cited by 55 publications

(49 citation statements)

References 29 publications

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“…Multiplex Fluorescent Array Assay for Assessing Direct IgE Antibody Binding-Monoclonal antibody 5H8 (20 g) was coupled to different Luminex carboxylated fluorescent microsphere bead sets (Luminex Corp., Austin, TX), and the multiplex fluorescent array was performed as described (34,35). The mAb-coupled beads were added along with the Group 1 mite allergens at a concentration (500 ng/ml) above the top of the dose-response curves to ensure that antibodies in the beads were bound to allergen.…”

mentioning

confidence: 99%

Molecular Determinants for Antibody Binding on Group 1 House Dust Mite Allergens

Chruszcz

Pomés

Glesner

et al. 2012

Journal of Biological Chemistry

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mentioning

confidence: 99%

Molecular Determinants for Antibody Binding on Group 1 House Dust Mite Allergens

Chruszcz

Pomés

Glesner

et al. 2012

Journal of Biological Chemistry

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“…Future attempts to incorporate a major share of allergens used in clinical assays into the VFM system will likely reveal such patterns. A considerable difference in performance between allergens has further been reported in other comparative studies, such as in a recently presented microarray assay with fluorescence-based detection (28 ).…”

Section: Evaluation Of a Vfm For Specific Ige Detection In A Clinicalmentioning

confidence: 82%

Point-of-Care Vertical Flow Allergen Microarray Assay: Proof of Concept

et al. 2014

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BACKGROUND: Sophisticated equipment, lengthy protocols, and skilled operators are required to perform protein microarray-based affinity assays. Consequently, novel tools are needed to bring biomarkers and biomarker panels into clinical use in different settings. Here, we describe a novel paper-based vertical flow microarray (VFM) system with a multiplexing capacity of at least 1480 microspot binding sites, colorimetric readout, high sensitivity, and assay time of Ͻ10 min before imaging and data analysis.

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“…This last panel with the inclusion of Amb a 1 would significantly improve pollen allergy diagnosis in North America. In this context, recent product diagnosis development such as multiplexed assay recently commercialized [77] might be of help.…”

Section: Sensitization and Clinical Relevancementioning

confidence: 99%

Challenges for Allergy Diagnosis in Regions with Complex Pollen Exposures

Barber

Díaz‐Perales

Villalba

et al. 2014

Curr Allergy Asthma Rep

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Over the past few decades, significant scientific progress has influenced clinical allergy practice. The biological standardization of extracts was followed by the massive identification and characterization of new allergens and their progressive use as diagnostic tools including allergen micro arrays that facilitate the simultaneous testing of more than 100 allergen components. Specific diagnosis is the basis of allergy practice and is always aiming to select the best therapeutic or avoidance intervention. As a consequence, redundant or irrelevant information might be adding unnecessary cost and complexity to daily clinical practice. A rational use of the different diagnostic alternatives would allow a significant improvement in the diagnosis and treatment of allergic patients, especially for those residing in complex pollen exposure areas.

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Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array

Cited by 55 publications

References 29 publications

Molecular Determinants for Antibody Binding on Group 1 House Dust Mite Allergens

Molecular Determinants for Antibody Binding on Group 1 House Dust Mite Allergens

Point-of-Care Vertical Flow Allergen Microarray Assay: Proof of Concept

Challenges for Allergy Diagnosis in Regions with Complex Pollen Exposures

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