Simultaneous detection of two growth factors from human single-embryo culture medium by a bead-based digital microfluidic chip

Lee, Meng-Shiue; Hsu, Wensyang; Huang, Hsiao‐Ping; Tseng, Hsueh Yang; Lee, Chia Tung; Hsu, Chung Yu; Shieh, Yi Chueh; Wang, Shih Hung; Yao, Da; Liu, Cheng-Hsien

doi:10.1016/j.bios.2019.111851

Cited by 34 publications

(30 citation statements)

References 42 publications

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“…hCG β could, hence, be helpful as a biomarker to evaluate the quality of embryos. The identification and measurement, during IVF, of human embryo growth factors named human IL-1β and human TNF-α are explained in [ 98 ].…”

Section: Microfluidics In Embryo Quality Analysismentioning

confidence: 99%

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

2021

Self Cite

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Infertility is a state of the male or female reproductive system that is defined as the failure to achieve pregnancy even after 12 or more months of regular unprotected sexual intercourse. Assisted reproductive technology (ART) plays a crucial role in addressing infertility. Various ART are now available for infertile couples. Fertilization in vitro (IVF), intracytoplasmic sperm injection (ICSI) and intrauterine insemination (IUI) are the most common techniques in this regard. Various microfluidic technologies can incorporate various ART procedures such as embryo and gamete (sperm and oocyte) analysis, sorting, manipulation, culture and monitoring. Hence, this review intends to summarize the current knowledge about the application of this approach towards cell biology to enhance ART.

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Section: Microfluidics In Embryo Quality Analysismentioning

confidence: 99%

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

2021

Self Cite

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“…The red-colored liquid is first moved to the center and is followed by moving blue-colored liquid to the center. The operation is designed as a concentration control in droplet-based applications [53]. Figure 8b shows the results from the mixing test.…”

Section: Droplet Mixing With Controllable Dropletmentioning

confidence: 99%

Universal Plasma Jet for Droplet Manipulation on a PDMS Surface towards Wall-Less Scaffolds

Peng

Tsai

2021

Polymers

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Droplet manipulation is important in the fields of engineering, biology, chemistry, and medicine. Many techniques, such as electrowetting and magnetic actuation, have been developed for droplet manipulation. However, the fabrication of the manipulation platform often takes a long time and requires well-trained skills. Here we proposed a novel method that can directly generate and manipulate droplets on a polymeric surface using a universal plasma jet. One of its greatest advantages is that the jet can tremendously reduce the time for the platform fabrication while it can still perform stable droplet manipulation with controllable droplet size and motion. There are two steps for the proposed method. First, the universal plasma jet is set in plasma mode for modifying the manipulation path for droplets. Second, the jet is switched to air-jet mode for droplet generation and manipulation. The jetted air separates and pushes droplets along the plasma-treated path for droplet generation and manipulation. According to the experimental results, the size of the droplet can be controlled by the treatment time in the first step, i.e., a shorter treatment time of plasma results in a smaller size of the droplet, and vice versa. The largest and the smallest sizes of the generated droplets in the results are about 6 L and 0.1 L, respectively. Infrared spectra of absorption on the PDMS surfaces with and without the plasma treatment are investigated by Fourier-transform infrared spectroscopy. Tests of generating and mixing two droplets on a PDMS surface are successfully achieved. The aging effect of plasma treatment for the proposed method is also discussed. The proposed method provides a simple, fast, and low-cost way to generate and manipulate droplets on a polymeric surface. The method is expected to be applied to droplet-based cell culture by manipulating droplets encapsulating living cells and towards wall-less scaffolds on a polymeric surface.

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“…In a recent work, using electrochemical impedance spectroscopy, hCGb, IL-8, and tumor necrosis factor a (TNFa) were detected from culture media of single human embryos: nonviable embryos produced higher levels of IL-8 and TNFa; interestingly, hCGb levels were able to discriminate between morphologically identical viable embryos (68). Another recent study used a bead-based digital microfluidic system to simultaneously measure IL-1b and TNFa in a single 520-nL droplet of culture medium in %40 minutes (87). The system consisted in an on-chip fluorescent immunoassay and used magnetic beads conjugated with specific capture antibodies and detection antibodies conjugated with a fluorescent compound.…”

Section: Measuring Metabolic Endpointsmentioning

confidence: 99%

Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation

Casciani¹,

Galliano

Franasiak

et al. 2021

F&S Reviews

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For decades, assisted reproductive technology (ART) procedures have been performed manually thanks to the meticulous work of skilled embryologists. Recently, new technologies have been developed with three main scopes: improving embryo culture conditions; making diagnostic evaluations more consistent and reliable; and allowing ART procedures to become progressively less subjective and operatordependent. This review aimed to answer the following questions: is automation likely to be successfully incorporated into the in vitro fertilization laboratory and clinical ART in the future? If so, would such automation result in improved outcomes in ART?An electronic search of PubMed was performed to identify articles in English language that addressed automation in ART. Studies were classified in decreasing categories: randomized controlled trials; prospective controlled trials; prospective noncontrolled trials; retrospective studies; and experimental studies. Research and development data from investigators were included.There are a number of separate platforms that have been developed until now to address different parts of the ART process: gradual change of embryo culture medium; noninvasive embryo monitoring with time-lapse or metabolome analysis; and automated vitrification. It is conceivable that future automation enhancements in the in vitro fertilization laboratory may improve consistency and throughput and reduce the risk of human error associated with the performance of repetitive tasks. Nevertheless, a need remains for a platform able to integrate all separate technologies, capable of successfully interconnecting them in a way that assures continued chain of custody for the gametes and embryos. Moreover, controlled trials will be fundamental to demonstrate the usefulness of automation in ART. (Fertil Steril Rev Ò 2021;2:251-64. Ó2021 by American Society for Reproductive Medicine.

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Simultaneous detection of two growth factors from human single-embryo culture medium by a bead-based digital microfluidic chip

Cited by 34 publications

References 42 publications

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

Universal Plasma Jet for Droplet Manipulation on a PDMS Surface towards Wall-Less Scaffolds

Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation

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