Simultaneous determination and pharmacokinetic study of six flavonoids from Fructus Sophorae extract in rat plasma by LC–MS/MS

Lu, Chang; Ren, Yi; Cao, Liang; Sun, Yuhui; Sun, Qian; Sheng, Ning; Lin, Yang; Zhi, Xuran; Zhang, Lantong

doi:10.1016/j.jchromb.2012.07.015

Cited by 39 publications

(19 citation statements)

References 16 publications

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“…Experimental data and pharmacokinetic parameters are expressed as means AE SD. Plasma and tissue homogenate concentrationtime curves were plotted and pharmacokinetic parameters Quantification of seven ingredients from Si-Ni-San extract in rats were calculated using Excel software by a noncompartmental method (Chang et al, 2012;Du et al, 2010). The pharmacokinetic parameters relating to K e (elimination rate constant) were estimated by linear regression of the plasma concentrations in the log-linear terminal phase; t 1/2 (biological half-life) was calculated as 0.693/K e ; AUC 0-t (area under curve from time 0 to the last measured concentration) and AUC 0-1 (area under the blood concentration-time curve from time zero to infinity) were calculated using software; and C max (maximum concentration) and T max (time to reach the maximum concentration) were obtained directly from a concentration-time curve.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous quantification of paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin from Si‐Ni‐San extract in rat plasma and tissues by liquid chromatography–tandem mass spectrometry

Tian-xue

Yan

Zhou

et al. 2013

Biomedical Chromatography

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In this study, a sensitive and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of seven bioactive components including paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin in rat plasma and tissues after oral administration of Si-Ni-San extract using astragaloside IV as internal standard (IS). The plasma and tissue samples were extracted by solid-phase extraction. Chromatographic separation was accomplished on a C18 column with a multiple-step gradient elution. The quantification was obtained by scanning with multiple reaction monitoring via an electrospray ionization source that was operated by switching between the positive and negative modes in two MS/MS scan segments. Full validation of the assay was implemented. In conclusion, this method demonstrated good linearity and specificity. The lower limits of quantification for the analytes were <7.5 ng/mL. Intra- and inter-day precisions (RSD) were <12.5% and accuracy (RE) ranged from -10.2 to 7.3%. The average recoveries of the analytes from rat plasma and tissues were >65.2% and 58.6%, respectively. The validated method was further applied to the determination of actual rat plasma and tissues after oral administration of Si-Ni-San extract. The results provided a meaningful basis for the clinical application of this prescription.

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Section: Discussionmentioning

confidence: 99%

Simultaneous quantification of paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin from Si‐Ni‐San extract in rat plasma and tissues by liquid chromatography–tandem mass spectrometry

Tian-xue

Yan

Zhou

et al. 2013

Biomedical Chromatography

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“…The current study revealed that some components in Fructus sophorae have hemostatic properties as well as anti-obesity, anti-tumor, anti-menopausal and anti-hemorrhoid effects (16–18). However, a limited amount of research has investigated whether Fructus sophorae has therapeutic effects on RA.…”

Section: Discussionmentioning

confidence: 81%

Protective effects of Fructus sophorae extract on collagen-induced arthritis in BALB/c mice

Han

Hong

Park

et al. 2016

Experimental and Therapeutic Medicine

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Styphnolobium japonicum (L.) is utilized in Korean medicine for the treatment of various inflammatory diseases. The aim of the present study was to explore the effects of Fructus sophorae extract (FSE) isolated from the dried ripe fruit of S. japonicum (L.) on the development of type II collagen-induced arthritis (CIA) in BALB/c mice. The CIA mice were orally administered FSE or saline daily for 2 weeks. The incidence and severity of disease and the inflammatory response in the serum and the joint tissues were assessed. Macroscopic and histological investigation indicated that FSE protected against CIA development. FSE was associated with a significant reduction in the levels of total immunoglobulin G2a and proinflammatory cytokines and mediators in the serum. In addition, FSE suppressed the gene expression levels of proinflammatory cytokines and mediators, the mediator of osteoclastic bone remodeling, the receptor activator of nuclear factor κ-B ligand and matrix metalloproteinases in the joint tissues. The present results suggest that FSE may protect against inflammation and bone damage, and would be a valuable candidate for further investigation as a novel anti-arthritic agent.

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“…The elimination half‐life ( T 1/2 ) was determined by the following equation: T 1/2 = 0.693/ k . The elimination rate constants ( k ) were measured using linear regression analysis of the logarithmic transformation of the last four points of the curve (Chang et al ., ). The value of area under the concentration–time curve (AUC 0 → t and AUC 0 → ∞ ) was used to evaluate drug exposure, which was calculated through the trapezoidal rule (Du et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Simultaneous determination of linarin, naringenin and formononetin in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of Bushen Guchi Pill

Guo

Dong

Yan

et al. 2014

Biomedical Chromatography

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A sensitive and reproducible liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of linarin, naringenin and formononetin in rat plasma after addition of sulfamethoxazole as the internal standard (IS). Separation was carried out on a Diamonsil C18 column (150 × 4.6 mm, 5 µm) with liner gradient elution using methanol (A) and 0.5‰ formic acid aqueous solution (B). Detection was performed on a triple-quadrupole linear ion trap mass spectrometer with the negative ion electrospray ionization in multiple-reaction monitoring (MRM) mode. The MRM transitions were m/z 591.2 → 283.2, 271.0 → 150.9, 266.9 → 252.0 and 252.0 → 155.9 for linarin, naringenin, formononetin and IS, respectively. All analytes showed good linearity within the concentration range (r > 0.9973). The lower limits of quantitation of linarin, naringenin and formononetin were 0.64, 1.07 and 1.04 ng/mL, respectively. Intra-day and inter-day precisions of the investigated components exhibited an RSD within 9.96%, and the accuracy (relative error) ranged from -11.25 to 9.38% at all quality control levels. The developed method was successfully applied to a pharmacokinetic study of linarin, naringenin and formononetin in rats after oral administration of Bushen Guchi Pill.

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Simultaneous determination and pharmacokinetic study of six flavonoids from Fructus Sophorae extract in rat plasma by LC–MS/MS

Cited by 39 publications

References 16 publications

Simultaneous quantification of paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin from Si‐Ni‐San extract in rat plasma and tissues by liquid chromatography–tandem mass spectrometry

Simultaneous quantification of paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin from Si‐Ni‐San extract in rat plasma and tissues by liquid chromatography–tandem mass spectrometry

Protective effects of Fructus sophorae extract on collagen-induced arthritis in BALB/c mice

Simultaneous determination of linarin, naringenin and formononetin in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of Bushen Guchi Pill

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