Simultaneous Determination and Pharmacokinetics Study of Three Triterpenes from Sanguisorba officinalis L. in Rats by UHPLC–MS/MS

Abstract

A selective and rapid ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was established and validated for the determination of ziyuglycoside I, 3β,19α-dihydroxyurs-12-en-28-oic-acid 28-β-d-glucopyranosyl ester, and pomolic acid in rats after the oral administration of ziyuglycoside I, 3β,19α-dihydroxyurs-12-en-28-oic-acid 28-β-d-glucopyranosyl ester, pomolic acid, and Sanguisorba officinalis L. extract. The separation was carried out on an ACQUITY UPLC®HSS T3 column (2.1 mm × 100 mm, 1.8 μm), using methanol and 5 mmol/L ammonium acetate water as the mobile phase. The three compounds were quantified using the multiple reaction monitoring mode with the electrospray ion source in both the positive and negative mode. Liquid-liquid extraction was applied to the plasma sample preparation. Bifendate was selected as the internal standard. The intra-day and inter-day precision and the accuracy of the method were all within receivable ranges. The lower limit of quantification of ziyuglycoside I, 3β,19α-dihydroxyurs-12-en-28-oic-acid 28-β-d-glucopyranosyl ester, and pomolic acid were 6.50, 5.75, and 2.63 ng/mL, respectively. The extraction recoveries of analytes in rat plasma ranged from 83 to 94%. The three components could be rapidly absorbed into the blood (Tmax, 1.4–1.6 h) both in the single-administration group or S. officinalis extract group, but the first peak of PA occurred at 0.5 h and the second peak at 4–5 h in the S. officinalis extract. Three compounds were eliminated relatively slowly (t1/2, 7.3–11 h). The research was to establish a rapid, sensible, and sensitive UHPLC–MS/MS method using the multi-ion mode for multi-channel simultaneous mensuration pharmacokinetics parameters of three compounds in rats after oral administration of S. officinalis extract. This study found, for the first time, differences in the pharmacokinetic parameters of the three compounds in the monomer compounds and S. officinalis extract administration, which preliminarily revealed the transformation and metabolism of the three compounds in vivo.

Simultaneous determination and pharmacokinetics study of three triterpenoid saponins in rat plasma by ultra‐high‐performance liquid chromatography tandem mass‐spectrometry after oral administration of Astragalus Membranaceus leaf extract

“…The sample processing methods and mobile phase were optimized to improve peak shape, stabilize retention time, increase the signal response of analytes, and shorten run time [15][16][17]. In this study, the influence of different mobile phase combinations (methanol-water, acetonitrile-water) and added buffers (formic acid, acetic acid, ammonium formate, and ammonium acetate) on the chromatographic behavior of the target compound was investigated.…”

Unveiling the Anti-Cancer Potential of Onoceranoid Triterpenes from Lansium domesticum Corr. cv. kokosan: An In Silico Study against Estrogen Receptor Alpha

“…Subsequently, we subjected the three onoceranoid triterpenes to pharmacokinetic prediction, including absorption, distribution, metabolism, and excretion properties. These pharmacokinetic criteria are essential in drug development, as they provide insights into the behavior of compounds in the body and their potential efficacy [43]. Additionally, the toxicity properties of the onoceranoid triterpenes were assessed, which is crucial for evaluating their safety and possible adverse effects [44].…”

Pharmacokinetics of Ziyuglycoside I and Ziyuglycoside II in Rat Plasma by UPLC-MS/MS

“…Ziyuglycoside I and ziyuglycoside II, isolated from Sanguisorba ofcinalis L., are the important active components of Sanguisorba ofcinalis L., which have excellent pharmacological efects. Many studies have shown that saponins extracted from Sanguisorba ofcinalis L. have antioxidant and anticancer efects [7][8][9]. Among other things, ziyuglycoside I could prevent the formation of collagen fbers, efectively promote the production of type I collagen, and reduce the generation of skin wrinkles, which could be used in the synthesis of beauty and skin care products [7,10].…”