Simultaneous determination and validation of antimicrobials in plasma and tissue of actinomycetoma by high-performance liquid chromatography with diode array and fluorescence detection

Cavazos-Rocha, Norma; Vera-Cabrera, Lucio; Welsh-Lozano, Oliverio; Waksman, Noemí; Salazar‐Cavazos, María de la Luz

doi:10.1016/j.jpba.2006.12.019

Cited by 26 publications

(10 citation statements)

References 25 publications

(61 reference statements)

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“…No adverse events occurred or were reported after a single 600 mg LZD and both formulations were well tolerated. [ 15 ] or with tandem LC-MS/MS [ 18 , 19 ] . However, most of these methods may require gradient elution mode [ 12 , 15 ] , complicated sample extraction procedures such as solid-phase extraction [ 9 , 10 , 15 ] , large sample volume [ 7 , 9 -19 ] , large retention time [ 7 ] or need expensive detectors (i. e., mass spectrometers) [ 18 , 19 ] not commonly available in clinical laboratories.…”

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confidence: 99%

Pharmacokinetics and Relative Bioavailability Evaluation of Linezolid Suspension and Tablet Formulations

Helmy

2013

Drug Res (Stuttg)

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The oral liquid formulations poses an alternative way in providing medications to pediatric patients, geriatric patients, patients with feeding tubes, and patients who cannot swallow solid dosage forms. This study was conducted to evaluate the pharmacokinetics (PKs) and relative bioavailability of suspension (reference) and tablet (test) formulations of Linezolid (LZD). In vivo study was established according to a single-center, randomized, single-dose, laboratory-blinded, 2 Way, Cross-Over Study with a washout period of 1-week. Under fasting conditions, 28 healthy Egyptian male volunteers were randomly allocated to receive a single oral dose of either 30 ml LZD or 1 tablet (600 mg LZD) of marketed suspension and tablet formulations. Plasma samples were obtained over a 48-h interval and analyzed for LZD by reversed phase liquid chromatography with ultraviolet detection. The 90% confidence intervals for the ratio of log transformed values of Cmax, AUC0-t, and AUCt-∞ of the two treatments were within the acceptable range (0.8-1.25) for bioequivalence. From PK perspectives, in this small study in healthy Egyptian adult male volunteers, a single 600 mg dose of the tablet formulation demonstrated comparable rate and extent of absorption to a single 600 mg dose of the suspension formulation based on the US FDA's regulatory definition. No adverse events occurred or were reported after a single 600 mg LZD and both formulations were well tolerated.

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confidence: 99%

Pharmacokinetics and Relative Bioavailability Evaluation of Linezolid Suspension and Tablet Formulations

Helmy

2013

Drug Res (Stuttg)

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“…Plasma concentrations were determined by using by using a previously validated HPLC (High Performance Liquid Chromatography) method as follows [13]. The serum protein was precipitated by combining 50 µL of sample with 150 µl of acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Effect of a Novel Oxazolidinone, DA-7867, in BALB/c Mice Infected with Nocardia brasiliensis

et al. 2008

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BackgroundMycetoma is a chronic infectious disease of tropical and subtropical countries. It is produced by true fungi and actinobacteria. In México, Nocardia brasiliensis is the main causative agent of mycetoma, producing about 86% of the cases; the gold standard for the therapy of mycetoma by N. brasiliensis is the use of sulfonamides which give a 70% cure rate. The addition of amikacin to this regime increases to 95% the cure rate; however, the patients have to be monitored for creatinine clearance and audiometry studies because of the potential development of side effects. Because of that it is important to search for new active compounds. In the present work, we evaluated the in vivo effect of DA-7867, an experimental oxazolidinone, on the development of experimental mycetomas by N. brasiliensis in BALB/c mice.Methodology/Principal FindingsIn order to determine the optimal dose utilized to apply to the animals, we first determined by HPLC the plasma levels using several concentrations of the compounds. Based on these results, we used 10 and 25 mg/kg subcutaneously every 24 hr; DA-7867 was also supplied in the drinking water at a calculated dose of 25 mg/kg. As a control we utilized linezolid at 25 mg/kg, a compound active in murine and human infections, three times a day. The mice were infected in the right footpad with a young culture of N. brasiliensis HUJEG-1, and one week later we started the application of the antimicrobials for six more weeks. After that we compared the development of lesions in the groups injected with saline solution or with the antimicrobials; the results were analyzed by the variance ANOVA test. DA-7867 was able to reduce the production of lesions at 25 mg/kg, when given either subcutaneously or in the drinking water.Conclusions/SignificanceThe experimental oxazolidinone DA-7867 is active in vivo against N. brasiliensis, which opens the possibility of using this drug once it is accepted for human application. Since oxazolidinones seem to be active against a wide spectrum of actinobacteria, it is possible they could be used in human cases of mycetoma by other actinomycetales, such as Streptomyces somaliensis, highly prevalent in Sudan, or Actinomadura madurae and A. pelletieri, which are commonly observed in Africa and India.

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“…Therefore, a suitable method for simultaneous determination of these drugs is required. Many HPLC methods are available for the measurement of amoxicillin [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] or sulbactam [22][23][24][25][26][27] in biological samples, individually [6][7][8][9][10][11][12] or in combination with other drugs or metabolites [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27]. In past, the plasma concentrations of amoxicillin and sulbactam are mainly measured via HPLC-UV or HPLC-mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of amoxicillin and sulbactam in human plasma by HPLC-DAD for assessment of bioequivalence

Pei

Yang

et al. 2011

Journal of Chromatography B

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Simultaneous determination and validation of antimicrobials in plasma and tissue of actinomycetoma by high-performance liquid chromatography with diode array and fluorescence detection

Cited by 26 publications

References 25 publications

Pharmacokinetics and Relative Bioavailability Evaluation of Linezolid Suspension and Tablet Formulations

Pharmacokinetics and Relative Bioavailability Evaluation of Linezolid Suspension and Tablet Formulations

Therapeutic Effect of a Novel Oxazolidinone, DA-7867, in BALB/c Mice Infected with Nocardia brasiliensis

Simultaneous analysis of amoxicillin and sulbactam in human plasma by HPLC-DAD for assessment of bioequivalence

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