Simultaneous determination of 12 steroids by isotope dilution liquid chromatography-photospray ionization tandem mass spectrometry

Guo, Tiedong; Taylor, Robert L.; Singh, Ravinder J.; Soldin, Steven J.

doi:10.1016/j.cca.2006.03.034

Cited by 204 publications

(177 citation statements)

References 16 publications

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“…At all three time points ten steroids were analyzed by MS/MS as previously described (24)(25)(26). Serum samples were deproteinized and injected onto the column after centrifugation.…”

Section: Laboratory Measurementsmentioning

confidence: 99%

“…Samples were washed online and eluted using a methanol/water gradient and introduced into the mass spectrometer. The current assay protocol (26) separates the total ion chromatogram into four sections in order to optimize parameters for each individual analyte. Atmospheric pressure photoionization was used to measure nine of the steroids in the positive ion mode; aldosterone was measured in the negative mode.…”

Section: Laboratory Measurementsmentioning

confidence: 99%

“…Our first generation steroid profile assay required 760 μL of serum and measured 9 steroids in 18 minutes (25). Our second generation assay uses 200 μL of serum and measures 12 steroids in 11 minutes (26). Assay accuracy was evaluated in two ways.…”

Section: Laboratory Measurementsmentioning

confidence: 99%

“…Assay accuracy was evaluated in two ways. The assays were compared with MS/MS assays performed at the Mayo Clinic: correlation coefficients ranged from 0.908 to 0.999 (26). Recovery studies were also performed and yielded mean recoveries of the analytes of 90% to 110% (26).…”

Section: Laboratory Measurementsmentioning

confidence: 99%

“…These nine hormones are cortisol, 11-deoxycortisol 17-hydroxyprogesterone, progesterone, androstenedione, DHEA, DHEA-S, estradiol, and testosterone. An additional refinement allows simultaneous of twelve steroids including aldosterone (26).…”

Section: Introductionmentioning

confidence: 99%

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Use of steroid profiles in determining the cause of adrenal insufficiency

et al. 2007

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Hypothesis-A cortisol response to adrenocorticotropin injection is the standard test for diagnosing adrenal insufficiency. Multiple steroid hormones can now be accurately measured by tandem mass spectrometry in a single sample. The study objective was to determine whether a steroid profile, created by simultaneous measurement of ten steroid hormones by tandem mass spectrometry, would help determine the cause of adrenal insufficiency.Design-A ten-steroid profile was measured by tandem mass spectrometry during the performance of a standard high dose cortrosyn stimulation test. The steroids were measured at baseline, and 30 and 60 minutes following synthetic adrenocorticotropin injection. Adrenal insufficiency was defined as a peak cortisol level of less than 20 μg/dL. Testing was conducted in the General Clinical Research Center of a University Medical Center. Normal volunteers, patients suspected of having adrenal insufficiency, and patients with known adrenal insufficiency participated.Results-Our results showed that adrenal insufficiency of any cause was adequately diagnosed using the response of 11-deoxycortisol, dehydroepiandrosterone, or these analytes combined in a two-steroid profile. A three-steroid profile yielded a test with 100% accuracy for discriminating primary adrenal insufficiency from normal status. Primary adrenal insufficiency was well separated from secondary adrenal insufficiency using only a single aldosterone value. 11-deoxycortisol, dehydroepiandrosterone, and a two-steroid profile each provided fair discrimination between secondary adrenal insufficiency and normal status.Conclusions-We conclude that stimulated levels of aldosterone, 11-deoxycortisol, dehydroepiandrosterone, and a two-or three-steroid profile provided additional discrimination between states of adrenal sufficiency and insufficiency. It is proposed that a steroid profile measuring

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“…At all three time points ten steroids were analyzed by MS/MS as previously described (24)(25)(26). Serum samples were deproteinized and injected onto the column after centrifugation.…”

Section: Laboratory Measurementsmentioning

confidence: 99%

Section: Laboratory Measurementsmentioning

confidence: 99%

Section: Laboratory Measurementsmentioning

confidence: 99%

Section: Laboratory Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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