“…The commonly used HPLC mobile phase for separation of oil-soluble dyes was acetonitrile or methanol containing 0.1% formic acid. We ever used methanol-acetone (9:1, v/v) containing 0.1% formic acid as mobile phase, and obtain efficient separation of 8 oil-soluble synthetic dyes, these dyes contain Para red, Sudan I-IV, Sudan red G, Sudan red B, Sudan red 7B, Sudan red G (Zhu, Li, Gong, Zhou, & Li, 2007). Sun et al (2007) reported the good separation of 10 azo-dyes (Para red, Sudan I-IV, Sudan red G, Sudan red B, Sudan red 7B, Sudan orange G, and Butter yellow) with methanol containing 0.1% formic acid and 0.1% formic acid aqueous solution as the mobile phase system under the gradient elution condition.…”