Simultaneous determination of 16 macrolide antibiotics and 4 metabolites in milk by using Quick, Easy, Cheap, Effective, Rugged, and Safe extraction (QuEChERS) and high performance liquid chromatography tandem mass spectrometry

Zhou, Wenying; Ling, Yun; Liu, Tong; Zhang, Yuan; Li, Jianzhi; Li, Hongna; Wu, Wenjie; Shou-jun, Jiang; Feng, Feng; Fang, Yuan; Zhang, Feng

doi:10.1016/j.jchromb.2017.07.046

Cited by 44 publications

(19 citation statements)

References 17 publications

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“…In order to reduce the effect of substrate on the substrate, matrix effect was investigated. It was investigated by calculating the percentage (C%) of signal enhancement or suppression according to equation :

C % = (1 - S s / S m) \times 100,

where Ss and Sm are the slope of matrix‐matched calibration solutions and calibration solutions in solvent, respectively.…”

Section: Resultsmentioning

confidence: 99%

Determination of 14 heterocyclic aromatic amines in meat products using solid‐phase extraction and supercritical fluid chromatography coupled to triple quadrupole mass spectrometry

Zhang

Zhou

et al. 2020

J of Separation Science

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A novel, simple, and sensitive method has been developed for simultaneous determination of 14 heterocyclic aromatic amines in meat product using solid-phase extraction combined with ultrahigh-performance supercritical fluid chromatography coupled to tandem quadrupole mass spectrometry. The analytes could be separated within 7 min and identified using their retention times and mass. The developed method was validated based on the linearity, limits of quantification, precision, and accuracy. The recovery ranged from 52.3 to 97.5% with an acceptable standard deviation, which is not higher than 6%. The limits of quantitation ranged from 0.03 to 0.17 µg/kg. The selectivity and sensitivity were satisfactory in multiple reaction monitoring mode. The method was applied to commercial meat products, and the results demonstrated that the novel method has potential for the analysis of the targets in food matrices. This is the first work reporting the simultaneous quantification of 14 heterocyclic aromatic amines by means of ultrahigh-performance supercritical fluid chromatography coupled to tandem quadrupole mass spectrometry. K E Y W O R D Sfood-derived hazardous compounds, heterocyclic aromatic amines, meat, supercritical fluid chromatography, triple quadrupole mass spectrometry 1372

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C % = (1 - S s / S m) \times 100,

where Ss and Sm are the slope of matrix‐matched calibration solutions and calibration solutions in solvent, respectively.…”

Section: Resultsmentioning

confidence: 99%

Determination of 14 heterocyclic aromatic amines in meat products using solid‐phase extraction and supercritical fluid chromatography coupled to triple quadrupole mass spectrometry

Zhang

Zhou

et al. 2020

J of Separation Science

Self Cite

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“…The average recovery (n = 6) of 31 target analytes obtained from the milk sample spiked with each target analyte at 100 µg/kg using ACN, methanol, 0.1 % formic acid with ACN, 0.1 % formic acid with 10 mmol/L ammonium acetate for the solvent extraction on the ionization of target substances, thus, affecting the quantitative determination of target substances. According to the formula: matrix effect ME (%) = (1-Ss/Sm) ×100 calculate the matrix effect of 31 macrolides, whereas Ss and Sm represented the slope of calibration plot with matrixmatched calibration solutions and the slope of calibration plot with calibration solutions in solvent, respectively [41]. The ME was generally acceptable or could be considered negligible if the absolute value of the ratio were less than 20%.…”

Section: Matrix Effectmentioning

confidence: 99%

Targeted analysis of six emerging derivatives or metabolites together with 25 common macrolides in milk using Quick, Easy, Cheap, Effective, Rugged and Safe extraction and ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry

Wang

Ling

Zhou

et al. 2020

J of Separation Science

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An analytical method for the determination of six emerging derivatives or metabolites together with 25 common macrolides antibiotics in milk by ultraperformance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry was established. The samples were purified with optimized Quick, Easy, Cheap, Effective, Rugged, Safe methods. The amounts of primarysecondary amine, C18, and sodium acetate adsorbent materials were optimized by response surface method to obtain the best purification effect. The chromatographic separation was carried out using the XBridge-C18 (2.1 × 100 mm, 3.5 µm, Waters) column with mobile phase of acetonitrile with 0.1% v/v formic acid-water solutions (containing 10 mmol/L ammonium acetate), separated by gradient elution. The instrument was operated in the detection mode of electrospray positive and negative ions with Full MS/data dependent MS 2 acquisition mode detection, external standard method was used for quantitative analysis. The limits of detection and limits of quantitation of 31 compounds were 0.1-0.5 µg/L and 0.5-2.0 µg/L, respectively. A total of 31 compounds performed a good linearity in the range of 1 to 200 µg/L, and the correlation coefficient was greater than 0.990. The spiked recoveries in milk samples were 81.07-110.1% and the relative standard deviation was less than 5.1%. The method was successful applied to actual sample testing in the market.

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“…The separation and detection characteristics of liquid chromatography tandem mass spectrometry (LC-MS/MS) make it ideal for the investigation of veterinary medication residues in complicated food matrices [ 21 ]. Many targeted analytical methods with high selectivity have been developed based on LC-MS or LC-MS/MS to detect MACs in fish, chicken, swine, bovine tissues, meat, eggs, and milk [ 6 , 22 , 23 , 24 , 25 , 26 ]. However, targeted methods can detect known compounds but cannot be used to identify unknown compounds [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Erythromycin and Clarithromycin Metabolites Formed in Chicken Liver Microsomes Using Liquid Chromatography–High-Resolution Mass Spectrometry

Wang

Nam

Kim

et al. 2021

Foods

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Nontargeted analysis can be used for the rapid screening and confirmatory analysis of veterinary drugs and their metabolites, which are important for the comprehensive safety evaluation of animal-derived foods. Here, a novel nontargeted screening approach based on liquid chromatography coupled with electrospray ionization–high-resolution mass spectrometry (LC/ESI–HR-MS) was developed to determine erythromycin, clarithromycin, and their metabolites in chicken liver microsomes. Erythromycin and clarithromycin were incubated in vitro in the presence of NADPH for 60 min to generate metabolites in chicken liver microsomes. After the incubation, the supernatant was extracted using ultrasonic shaking, orbital shaking, and centrifugation before analysis using LC/ESI-HR-MS in positive ion mode on an Agilent Eclipse Plus C18 column (100 mm × 2.1 mm; i.d. 3.5 µm) with 0.1 percent formic acid-water and acetonitrile as the mobile phases for gradient elution at 0.4 mL/min. The results show that erythromycin can produce N-desmethyl-erythromycin A in chicken liver microsomes, but clarithromycin cannot produce N-desmethyl-clarithromycin in chicken liver microsomes. The N-desmethyl-erythromycin A and N-desmethyl-clarithromycin were tentatively identified in chicken liver microsomes using the established quick analytic method, which will provide a theoretical foundation for future research on pharmacokinetics and drug elimination in poultry.

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Simultaneous determination of 16 macrolide antibiotics and 4 metabolites in milk by using Quick, Easy, Cheap, Effective, Rugged, and Safe extraction (QuEChERS) and high performance liquid chromatography tandem mass spectrometry

Cited by 44 publications

References 17 publications

Determination of 14 heterocyclic aromatic amines in meat products using solid‐phase extraction and supercritical fluid chromatography coupled to triple quadrupole mass spectrometry

Determination of 14 heterocyclic aromatic amines in meat products using solid‐phase extraction and supercritical fluid chromatography coupled to triple quadrupole mass spectrometry

Targeted analysis of six emerging derivatives or metabolites together with 25 common macrolides in milk using Quick, Easy, Cheap, Effective, Rugged and Safe extraction and ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry

Identification of Erythromycin and Clarithromycin Metabolites Formed in Chicken Liver Microsomes Using Liquid Chromatography–High-Resolution Mass Spectrometry

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