Simultaneous determination of acetaminophen, dextromethorphen hydrobromide and pseudoephedrine hydrochloride in a new drug formulation for cold treatment by HPLC

Abstract

Key WardsColumn hquicl chromatography Acetaminophen Pseucloepheclrine hyclrochloricle Dextromethorphen hyclrobromicle Cold formulation SummaryA rapid and accurate HPLC method is described for the simultaneous determination of acetaminophen, clextromethorphen hyclrobromicle and pseucloepheclrine hyclrochloricle in a new cold formulation. Chromatographic separation of the three pharmaceuticals was performed on a Hypersil CN column (150 • 5.0 mm, 5 Ixm) with a mobile phase mixture of an ion-pairing solution, methanol and acetonitrile (25:57:18, v/v), at a flow rate of 1.0 mL min 1, with detection at 220 nm. Separation was complete in less than 10 min. The method was vahclatecl for hnearity, accuracy, precision, limit of quantitation and robustness. Linearity, accuracy, and precision were found to be acceptable over the ranges of 2.06 ~ 20.6 I~g 9 mL 1 for acetaminophen, 0.202 ~ 2.02 mg 9 mL 1 for pseucloepheclrine hyclrochloricle and 0.042 1.06 mg 9 mL 1 forclextromethorphen hyclrobromicle.

Chemometrically optimized micellar liquid chromatographic method for the simultaneous determination of cetirizine dihydrochloride in its combined dosage forms. Application to biological fluids and pharmacokinetic studies

“…Several HPLC methods have been reported in literature for the determination of PCA with CTZ [4,5], PCA with PSU [6][7][8][9], and CTZ with PSU [10][11][12]. The three drugs were also determined in the presence of their degradation products [13].…”