Simultaneous determination of aflatoxin M1, ochratoxin A, zearalenone and α-zearalenol in milk by UHPLC–MS/MS

Huang, Lingli; Zheng, Nan; Zheng, Bingjing; Fang, Wen; Cheng, Jianbo; Han, Rongwei; Xu, Xiaorong; Li, S.L.; Wang, J.Q.

doi:10.1016/j.foodchem.2013.09.047

Cited by 181 publications

(102 citation statements)

References 32 publications

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“…All the containers kept dry and sterile in the whole process [15] . Every sample was collected into three bottles.…”

Section: Sampling Proceduresmentioning

confidence: 99%

“…Two of them were used in the paralleled detection , and the third one would be preserved for three months just in case. After being well mixed and completely incorporated, 200 mL raw milk in each bottle was recollected from the top, middle, and bottom of its container, respectively [15] . Then 300 mL raw milk was removed and put into sterile sampling bottles, which was stored at 4 °C during our three hours' sampling trip [9,16].…”

Section: Sampling Proceduresmentioning

confidence: 99%

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Determination of Heavy Metals in Raw Milk Produced in Tangshan City, China

Zheng³

et al. 2016

MATEC Web of Conferences

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Abstract. The objective of this study was to investigate the content of heavy metals (Arsenic, Lead, Chromium and Mercury) in raw milk of Tangshan. Samples were obtained directly from dairy farms in Tangshan City. A total of 55 raw milk samples were collected in 2012, a total of 150 raw milk samples and 150 in 2013 and 2014 respectively. All the samples were analyzed by ICP-MS (Inductively Coupled Plasma Mass Spectrometry) method-based on China National Standard. In general, heavy metals (Arsenic, Lead, Chromium and Mercury) of raw milk in Tangshan were relatively safe for people's health because none of those heavy metals (Arsenic, Lead, Chromium and Mercury) contents exceeded the Maximum Limit set by China.

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“…All the containers kept dry and sterile in the whole process [15] . Every sample was collected into three bottles.…”

Section: Sampling Proceduresmentioning

confidence: 99%

Section: Sampling Proceduresmentioning

confidence: 99%

Determination of Heavy Metals in Raw Milk Produced in Tangshan City, China

Zheng³

et al. 2016

MATEC Web of Conferences

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“…The validated method is capable of detecting AFM1 at this level. It is also capable of detecting other mycotoxins that have been found in milk worldwide, such as aflatoxins G1, G2, B1, B2 [22], ZEA [23][24][25], DOM-1 [26], FB1 [27,28] and OTA [24,[29][30][31]; and others that have not been previously studied in milk such as OTB.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Mycotoxins in Peruvian Evaporated Cow Milk

Flores-Flores

González-Peñas

2018

Beverages

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Mycotoxins-toxic secondary fungi metabolites-reach humans through food, producing several effects on their health and economic losses. Mycotoxin co-occurrence is common in food due to the co-presence of different fungi species, each of which may produce different toxins. A survey regarding the presence of 22 mycotoxins (aflatoxins M1, B1, B2, G1, G2; ochratoxins A and B; fumonisins B1, B2 and B3; HT-2 and T-2 toxins; nivalenol; deoxynivalenol; deepoxy-deoxynivalenol; 3 and 15 acetyl-deoxynivalenol; diacetoxyscirpenol; fusarenon X; neosolaniol; sterigmatocystin; and zearalenone) in 30 Peruvian evaporated cow milk samples is presented for the first time. Analysis was carried out by liquid chromatography coupled to tandem mass spectrometry, which was based on two previously validated methods for quantification of these toxic compounds in liquid cow milk, and further validated for the new matrix. The only detected mycotoxin was ochratoxin A, which was found in four samples, although at levels below its limit of quantification (0.2 ng/mL). This initial study indicates that the presence of mycotoxins in evaporated milk is low in Peru. However, we recommend the analysis of more samples and more milk types obtained from urban and rural areas, in order to obtain more data that will allow further risk assessments to be carried out.

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“…Hang et al determined AFM 1 , ochratoxin A, zearalenone and α-zearalenol in milk by UHPLC-ESI-MS/MS after Oasis HLB cartridge cleanup. The LODs of the mycotoxins were in the range of 0.003-0.015 μg kg -1 (Huang et al 2014). Beltrán et al (2011) developed a UHPLC-MS/MS method to determinate aflatoxins G 1 , G 2 , B 1 , B 2 , M 1 and ochratoxin A in baby food and milk based on SPE with immunoaffinity columns; the LODs ranged from 1 to 8 ng kg -1 .…”

Section: Methods Comparisonmentioning

confidence: 99%

Simultaneous determination of aflatoxin B₁and M₁in milk, fresh milk and milk powder by LC-MS/MS utilising online turbulent flow chromatography

Fan

Sun

et al. 2015

Food Additives & Contaminants: Part A

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A novel, fully automated method based on dual-column switching using online turbulent flow chromatography followed by LC-MS/MS was developed for the determination of aflatoxin B1 and M1 in milk, fresh milk and milk powder samples. After ultrasound-assisted extraction, samples were directly injected into the chromatographic system and the analytes were concentrated on the clean-up loading column. Through purge switch, analytes were transferred to the analytical column for subsequent detection by mass spectrometry. Different types of TurboFlow(TM) columns, transfer flow rates and transfer times were optimised. Method limits of detection obtained for AFB1 and AFM1 were 0.05 μg kg(-1), and limits of quantification were 0.1 μg kg(-1). Recoveries of aflatoxin B1 and M1 were in range of 81.1-102.1% for all samples. Matrix effects of aflatoxin B1 and M1 were in range of 63.1-94.3%. The developed method was successfully used for the analysis of aflatoxin B1 and M1 in real samples.

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Simultaneous determination of aflatoxin M1, ochratoxin A, zearalenone and α-zearalenol in milk by UHPLC–MS/MS

Cited by 181 publications

References 32 publications

Determination of Heavy Metals in Raw Milk Produced in Tangshan City, China

Determination of Heavy Metals in Raw Milk Produced in Tangshan City, China

Analysis of Mycotoxins in Peruvian Evaporated Cow Milk

Simultaneous determination of aflatoxin B₁and M₁in milk, fresh milk and milk powder by LC-MS/MS utilising online turbulent flow chromatography

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Simultaneous determination of aflatoxin M1, ochratoxin A, zearalenone and α-zearalenol in milk by UHPLC–MS/MS

Cited by 181 publications

References 32 publications

Determination of Heavy Metals in Raw Milk Produced in Tangshan City, China

Determination of Heavy Metals in Raw Milk Produced in Tangshan City, China

Analysis of Mycotoxins in Peruvian Evaporated Cow Milk

Simultaneous determination of aflatoxin B1and M1in milk, fresh milk and milk powder by LC-MS/MS utilising online turbulent flow chromatography

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Product

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Simultaneous determination of aflatoxin B₁and M₁in milk, fresh milk and milk powder by LC-MS/MS utilising online turbulent flow chromatography