Simultaneous determination of amphetamine-type stimulants in abusers’ hair: clinical usefulness of hair analysis in prehospitalization for abusers

Wada, Mitsuhiro; Sugimoto, Yuki; Crabtree, Brian L.; Evans, Cynthia A.; Montgomery, Jenny; Ikeda, Rie; Kuroda, Naotaka; Nakashima, Kenichiro

doi:10.1007/s11419-012-0153-6

Cited by 12 publications

(2 citation statements)

References 20 publications

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“…Badano także włosy w przebiegu farmakoterapii padaczki, gdzie stwierdzono korelację pomiędzy wielkością dziennej dawki karbamazepiny czy fenobarbitalu a zawartością tych leków w jednocentymetrowych segmentach włosów odpowiadających jednemu miesiącowi. Taka analiza daje możliwość bieżącej obserwacji terapii pacjenta [32].…”

Section: Kliniczne Zastosowania Analizy Włosówunclassified

Wartość Diagnostyczna Włosów

Łuczak-Zielkiewicz¹,

Szutowski²

2013

pps

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Analiza włosów odgrywa obecnie coraz większą rolę w oznaczeniach toksykologicznych. Stosowana jest w medycynie sądowej, medycynie klinicznej, testach kierowców, monitoringu pracowników. W porównaniu do najczęściej stosowanych materiałów analitycznych, jak krew czy mocz, długi czas obecności substancji we włosach pozwala na oznaczenie jej po kilku, a nawet kilkunastu miesiącach od ekspozycji na dany czynnik. Analiza retrospektywna umożliwia poznanie historii pacjenta, stosowanych leków, rozpoznanie przypadków nadużywania substancji pobudzających, nieprzestrzegania abstynencji od alkoholu i innych środków uzależniających oraz pozwala zidentyfikować przyczynę zatruć. Szybki rozwój techniki sprawił, że dysponujemy coraz doskonalszymi metodami analitycznymi. Czułość współczesnych metod daje możliwość oznaczeń śladowych ilości (rzędu pikogramów) ksenobiotyków i ich metabolitów we włosach.

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Section: Kliniczne Zastosowania Analizy Włosówunclassified

Wartość Diagnostyczna Włosów

Łuczak-Zielkiewicz¹,

Szutowski²

2013

pps

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“…Amphetamines such as amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) continue to be listed among the most commonly used recreational drugs worldwide, including China, Japan [1][2][3][4][5], Korea [6], the United States [7,8], Portugal [9], and South Africa [10]. Precise and rapid determination of these compounds in biological samples is often essential, as this can provide crucial information for clinical and forensic toxicologists in determining the cause of impairment and/or death.…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid analysis of four amphetamines in human whole blood and urine using liquid–liquid extraction without evaporation/derivatization and gas chromatography–mass spectrometry

et al. 2014

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We developed a simple and rapid analysis method for the determination of four amphetamines in human whole blood and urine using gas chromatographymass spectrometry (GC-MS). During the course of sample preparation, the evaporation process was found to be the weak point for amphetamine and methamphetamine analysis. Liquid-liquid extraction in this study was conducted without the evaporation step, and the upper solvent layer was injected directly without derivatization, which effectively prevented the volatile amphetamines from vanishing during evaporation and guaranteed the linearity of the calibration curves. This method showed good selectivity, precision, and accuracy, and at the same time was simple and fast. The limits of detection were 5 ng/ml. As an application of this study, a whole blood sample and urine sample from a drug abuse suspect were analyzed. This method may be able to meet the need for rapid and accurate confirmation of positive immunoassay results as well as quantification in clinical and forensic toxicological cases. To our knowledge, no forensic toxicologists have noted that such a basic and simple method, without evaporation or derivatization, is useful for routine analysis of amphetamines using GC-MS.

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Simultaneous determination of 75 abuse drugs including amphetamines, benzodiazepines, cocaine, opioids, piperazines, zolpidem and metabolites in human hair samples using liquid chromatography–tandem mass spectrometry

Shin

Kong

Cheong

et al. 2019

Biomedical Chromatography

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A liquid chromatography–tandem mass spectrometric method for the simultaneous determination of 75 abuse drugs and metabolites, including 19 benzodiazepines, 19 amphetamines, two opiates, eight opioids, cocaine, lysergic acid diethylamide, zolpidem, three piperazines and 21 metabolites in human hair samples, was developed and validated. Ten‐milligram hair samples were decontaminated, pulverized using a ball mill, extracted with 1 mL of methanol spiked with 28 deuterated internal standards in an ultrasonic bath for 60 min at 50°C, and purified with Q‐sep dispersive solid‐phase extraction tubes. The purified extracts were evaporated to dryness and the residue was dissolved in 0.1 mL of 10% methanol. The 75 analytes were analyzed on an Acquity HSS T3 column using gradient elution of methanol and 0.1% formic acid and quantified in multiple reaction monitoring mode with positive electrospray ionization. Calibration curves were linear (r ≥ 0.9951) from the lower limit of quantitation (2–200 pg/mg depending on the drug) to 2000 pg/mg. The coefficients of variation and accuracy for intra‐ and inter‐assay analysis at three QC levels were 4.3–12.9% and 89.2–109.1%, respectively. The overall mean recovery ranged from 87.1 to 105.3%. This method was successfully applied to the analysis of 11 forensic hair samples obtained from drug abusers.

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Simultaneous determination of amphetamine-type stimulants in abusers’ hair: clinical usefulness of hair analysis in prehospitalization for abusers

Cited by 12 publications

References 20 publications

Wartość Diagnostyczna Włosów

Wartość Diagnostyczna Włosów

Simple and rapid analysis of four amphetamines in human whole blood and urine using liquid–liquid extraction without evaporation/derivatization and gas chromatography–mass spectrometry

Simultaneous determination of 75 abuse drugs including amphetamines, benzodiazepines, cocaine, opioids, piperazines, zolpidem and metabolites in human hair samples using liquid chromatography–tandem mass spectrometry

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