Simultaneous Determination of Caffeine and Paracetamol in Commercial Formulations Using Greener Normal-Phase and Reversed-Phase HPTLC Methods: A Contrast of Validation Parameters

Alam, Prawez; Shakeel, Faiyaz; Ali, Abuzer; Alqarni, Mohammed H.; Foudah, Ahmed I.; Aljarba, Tariq M.; Alkholifi, Faisal K.; Alshehri, Sultan; Ghoneim, Mohammed M.; Ali, Amena

doi:10.3390/molecules27020405

Cited by 29 publications

(22 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the current procedure, the blank solution (without TNX) was assessed using six replicates ( n = 6) and the standard deviation was computed. The standard deviation and slope of the TNX calibration plot were then used to get the data for TNX’s LOD and LOQ using the formulas that were provided. , …”

Section: Methodsmentioning

confidence: 99%

Eco-Friendly High-Performance Thin-Layer Chromatography Method for the Determination of Tenoxicam in Commercial Formulations

Shakeel,

Alam,

Haq

et al. 2023

ACS Omega

Self Cite

View full text Add to dashboard Cite

There is a dearth of information in the literature regarding environmentally benign high-performance thin-layer chromatography (HPTLC) methods to determine tenoxicam (TNX). Therefore, designing and validating an HPTLC method to detect TNX in commercial tablets and capsules was the goal of this investigation. The green mobile phase utilized was the combination of ethanol/water/ammonia solution (50:45:5 v/v/v). The TNX was quantified at a wavelength of 375 nm. The proposed method's greenness profile was established using the Analytical GREEnness (AGREE) approach. The proposed methodology for determining TNX was linear in the range of 25−1400 ng/band. The proposed methodology for measuring TNX was accurate (% recoveries = 98.24−101.48), precise (% RSD = 0.87−1.02), robust (% RSD = 0.87−0.94), sensitive (LOD = 0.98 ng/band and LOQ = 2.94 ng/band), and environmentally friendly. The AGREE scale for the present methodology was derived to be 0.75, indicating an outstanding greenness profile. TNX was found to be highly stable under acidic, base, and thermal stress conditions. However, it completely decomposed under oxidative stress conditions. Commercial tablets and capsules were found to have 98.46 and 101.24% TNX, respectively. This finding supports the validity of the current methodology for measuring TNX in commercial formulations. The outcomes of this work showed that the proposed eco-friendly HPTLC methodology can be used for the routine analysis of TNX in commercial formulations.

show abstract

Section: Methodsmentioning

confidence: 99%

Eco-Friendly High-Performance Thin-Layer Chromatography Method for the Determination of Tenoxicam in Commercial Formulations

Shakeel,

Alam,

Haq

et al. 2023

ACS Omega

Self Cite

View full text Add to dashboard Cite

show abstract

“…MeOH was used to extract a weight of powder equivalent to 5.0 mg of CLH. MeOH is the most effective solvent for the extraction, giving the highest extraction yields and highest content of phenolics, flavonoids, alkaloids, and terpenoids [47][48][49]. Lower temperature is needed for evaporation using MeOH extracts [47].…”

Section: Sample Preparation For the Determination Of Clh In Commercia...mentioning

confidence: 99%

“…MeOH is the most effective solvent for the extraction, giving the highest extraction yields and highest content of phenolics, flavonoids, alkaloids, and terpenoids [47][48][49]. Lower temperature is needed for evaporation using MeOH extracts [47]. CLH is an alkaloidal compound and hence MeOH is the most effective solvent to obtain the highest extraction yields of CLH from C. autumnale [3].…”

Section: Sample Preparation For the Determination Of Clh In Commercia...mentioning

confidence: 99%

See 1 more Smart Citation

Determination of Colchicine in Pharmaceutical Formulations, Traditional Extracts, and Ultrasonication-Based Extracts of Colchicum autumnale Pleniflorum (L.) Using Regular and Greener HPTLC Approaches: A Comparative Evaluation of Validation Parameters

Alqarni

Shakeel

Aljarba

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

In the literature, there is a scarcity of greener analytical approaches for colchicine (CLH) analysis. As a result, efforts were made in this study to develop and validate a greener reversed-phase high-performance thin-layer chromatography (HPTLC) technique for CLH analysis in traditional extracts (TE) and ultrasonication-based extracts (UBE) of commercial Unani formulations, commercial allopathic formulations, and Colchicum autumnale Pleniflorum (L.) obtained from Egypt and India. This new technique was compared to the regular normal-phase HPTLC method. The greenness profile of both methods was estimated using the Analytical GREENness (AGREE) approach. In the 100–600 and 25–1200 ng/band ranges, regular and greener HPTLC procedures were linear for CLH analysis, respectively. For CLH analysis, the greener HPTLC method was more sensitive, accurate, precise, and robust than the regular HPTLC method. For CLH analysis in TE and UBE of commercial Unani formulations, commercial allopathic formulations, and C. autumnale obtained from Egypt and India, the greener HPTLC method was superior in terms of CLH content compared to the regular HPTLC method. In addition, the UBE procedure was superior to the TE procedure for both methods. The AGREE scores for regular and greener reversed-phase HPTLC methods were found to be 0.46 and 0.75, respectively. The AGREE results showed excellent greener profile of the greener HPTLC method over the regular HPTLC technique. Based on several validation criteria and pharmaceutical assay findings, the greener HPTLC method is regarded as superior to the regular HPTLC approach.

show abstract

“…Several analytical techniques have been employed for the identification and quantification of major coffee metabolites, such as caffeine and trigonelline. These techniques have ranged from simple planar chromatography [ 9 , 10 , 11 ] to the use of more advanced techniques that are based on holistic metabolomics data analysis for the discrimination of specialty coffee commodities, including Luwak coffee, from other ordinary coffee-adulterant commodities. These include mass spectrometry (MS) coupled with chromatography, inductively coupled plasma atomic emission spectrometry (ICP-AES), Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, nuclear magnetic resonance (NMR), and GC with FID detection [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolite Profiling of Premium Civet Luwak Bio-Transformed Coffee Compared with Conventional Coffee Types, as Analyzed Using Chemometric Tools

et al. 2023

View full text Add to dashboard Cite

Luwak (civet) coffee is one of the most precious and exotic coffee commodities in the world. It has garnered an increasing reputation as the rarest and most expensive coffee, with an annual production. Many targeted analytical techniques have been reported for the discrimination of specialty coffee commodities, such as Luwak coffee, from other ordinary coffee. This study presents the first comparative metabolomics approach for Luwak coffee analysis compared to other coffee products, targeting secondary and aroma metabolites using nuclear magnetic resonance (NMR), gas chromatography (GC), or liquid chromatography (LC) coupled with mass spectrometry (MS). Chemometric modeling of these datasets showed significant classification among all samples and aided in identifying potential novel markers for Luwak coffee from other coffee samples. Markers have indicated that C. arabica was the source of Luwak coffee, with several new markers being identified, including kahweol, chlorogenic acid lactones, and elaidic acid. Aroma profiling using solid-phase micro-extraction (SPME) coupled with GC/MS revealed higher levels of guaiacol derivatives, pyrazines, and furans in roasted Luwak coffee compared with roasted C. arabica. Quantification of the major metabolites was attempted using NMR for Luwak coffee to enable future standardization. Lower levels of alkaloids (caffeine 2.85 µg/mg, trigonelline 0.14 µg/mg, and xanthine 0.03 µg/mg) were detected, compared with C. arabica. Other metabolites that were quantified in civet coffee included kahweol and difurfuryl ether at 1.37 and 0.15 µg/mg, respectively.

show abstract

Simultaneous Determination of Caffeine and Paracetamol in Commercial Formulations Using Greener Normal-Phase and Reversed-Phase HPTLC Methods: A Contrast of Validation Parameters

Cited by 29 publications

References 33 publications

Eco-Friendly High-Performance Thin-Layer Chromatography Method for the Determination of Tenoxicam in Commercial Formulations

Eco-Friendly High-Performance Thin-Layer Chromatography Method for the Determination of Tenoxicam in Commercial Formulations

Determination of Colchicine in Pharmaceutical Formulations, Traditional Extracts, and Ultrasonication-Based Extracts of Colchicum autumnale Pleniflorum (L.) Using Regular and Greener HPTLC Approaches: A Comparative Evaluation of Validation Parameters

Metabolite Profiling of Premium Civet Luwak Bio-Transformed Coffee Compared with Conventional Coffee Types, as Analyzed Using Chemometric Tools

Contact Info

Product

Resources

About