Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection

Liu, Guanshu; Chen, Junnan; Ma, Yinfa

doi:10.1016/j.jchromb.2004.03.011

Cited by 53 publications

(23 citation statements)

References 63 publications

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“…The average amount of His and Spd in single HeLa cell is estimated to be 0.7 and 0.28 fmol, respectively. As has been reported by Zhang et al [3] and Liu et al [38], Spd was also detected in PC12 cells with a content of 1.16 a) x, concentration of amines (mM); y, peak area of amine derivatives in electropherogram. b) PA, peak area; MT, migration time.…”

Section: Linear Range Reproducibility and Lodssupporting

confidence: 75%

Determination of biogenic amines in HeLa cell lysate by 6‐oxy‐(N‐succinimidyl acetate)‐9‐(2'–methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser‐induced fluorescence detection

Cao

Wang

et al. 2006

Electrophoresis

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An MEKC-LIF method using 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxy-carbonyl) fluorescein (SAMF) newly synthesized in our lab as a labeling reagent for the separation and determination of eight typical biogenic amines was proposed. After careful study of the derivatization condition such as pH value, reagent concentration, temperature, and reaction time, derivatization reaction was accomplished as quickly as 10 min with stable yield. Optimal separation of SAMF-labeled amines was achieved with a running buffer (pH 9.3) containing 30 mM boric acid, 25 mM SDS, and 20% v/v ACN. The proposed method allowed biogenic amines to be determined with LODs as low as 0.25-2.5 nmol/L and RSD values from 0.4 to 4.5%. The present method has been successfully used to monitor biogenic amines in HeLa cells and fish samples. This study exploits the potential of MEKC-LIF with SAMF labeling as a tool for monitoring biogenic amines involved in complex physiological and behavioral processes in various matrices.

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Section: Linear Range Reproducibility and Lodssupporting

confidence: 75%

Determination of biogenic amines in HeLa cell lysate by 6‐oxy‐(N‐succinimidyl acetate)‐9‐(2'–methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser‐induced fluorescence detection

Cao

Wang

et al. 2006

Electrophoresis

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“…Thus, the resolution of primary amino compounds was greatly improved by incorporating crown ethers into the BGE [125]. In this way, Liu et al [126] showed the resolution of polyamines by SDS-MEKC to be significantly improved by the presence of 18-crown-6 through the formation of a selective host-guest complex between a primary amino group in the polyamine and the crown ether. The ensuing method allows the simultaneous determination of catecholamines and polyamines in PC-12 cell extracts with LODs at the 10 27 mol/L level.…”

Section: Employment Of Coupled Equilibriamentioning

confidence: 99%

MEKC: An update focusing on practical aspects

Silva

2007

Electrophoresis

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This paper reviews recent methodological and instrumental advances in MEKC. Improvements in sensitivity arising from the use of on-line sample concentration (sweeping, stacking, and combination of both protocols) and derivatization (in-capillary reactions and coupling with flow-injection systems) and improvements in resolution obtained by changing the composition of the BGE (e.g., with organic modifiers, ionic liquids, nonionic and zwitterionic surfactants, mixed micelles, and vesicles) or using coated capillaries are discussed in detail. In addition, MS and LIF spectroscopy are examined in relation to their advantages and restrictions as applied to MEKC analysis. Some thoughts on potential future directions are also expressed.

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“…5. Boric acid buffer(100 mM, pH 9.5) containing 10 mM SDS and 10 mM 18-crown-6 is a useful BGE for the determination of catecholamines (5-HT, DA, E, NE) and polyamines (PUT, SPD, SPM) in PC-12 cell extracts by MEKC with UV-Vis absorption detection [180]. In the presence of SDS and 18-crown-6, the analytes were well resolved within 20 min.…”

Section: Applicationsmentioning

confidence: 99%

Analysis of biologically active amines by CE

et al. 2006

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This paper provides an overview on the current status of the analysis of biogenic amines by CE. The basic CE separation and detection strategies for the analysis of biogenic amines are briefly described. CZE and MEKC that provide highly efficient and reproducible analysis of biogenic amines are particularly surveyed. With respect to the detection of biogenic amines, we focus on LIF, UV-visible absorption, electrochemiluminescence, and MS. Derivatization strategies, indirect methods, and on-line concentration techniques such as field-amplified sample stacking, sweeping, and use of polymer solution are described. To show the practicality of CE, we highlight currently developed techniques for the determinations of biogenic amines in biological samples, including foods, beverages, cerebrospinal fluids, urine, and single cells.

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Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection

Cited by 53 publications

References 63 publications

Determination of biogenic amines in HeLa cell lysate by 6‐oxy‐(N‐succinimidyl acetate)‐9‐(2'–methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser‐induced fluorescence detection

Determination of biogenic amines in HeLa cell lysate by 6‐oxy‐(N‐succinimidyl acetate)‐9‐(2'–methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser‐induced fluorescence detection

MEKC: An update focusing on practical aspects

Analysis of biologically active amines by CE

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