Simultaneous Determination of Creatine Compounds and Adenine Nucleotides in Myocardial Tissue by High-Performance Liquid Chromatography

A Colorimetric Assay for the Quantitation of Free Adenine Applied to Determine the Enzymatic Activity of Ribosome-Inactivating Proteins

“…The toxins (RTA, dianthin, saporin and mutants) in the concentration range 0.5-50 pmol were mixed with 20A 260 units of 60S ribosomal subunits, 50 g 28S rRNA or with 100 g of either hsDNA, mtDNA, or poly(A). Samples were adjusted to 50 l (200 l for 60S) with the appropriate substrate buffer (20 mM Tris, 100 mM NH 4 Cl, 1 mM MgCl 2 , pH 7.5, for 60S subunits; 50 mM sodium acetate, 160 mM KCl, 1.25 mM magnesium acetate, pH 4.0, for hsDNA, 28S rRNA, and mtDNA; 20 mM sodium acetate, 10 mM magnesium acetate, 100 mM NH 4 Cl, pH 6, for poly(A) (23)) and incubated for 2 h. For calibration, 0.2 to 20 nmol of adenine in 15 l of 5 mM Tris (pH 7.5) was used instead of the toxins under otherwise identical conditions. To remove the substrate after toxin-mediated adenine release, 100 l (400 l for 60S) ethanol (Ϫ20°C) was added to the reaction mixture.…”

Preischemic hyperglycemia‐aggravated damage: Evidence that lactate utilization is beneficial and glucose‐induced corticosterone release is detrimental

“…Lactate and glucose were analyzed as described elsewhere (Schurr et al, 1997a(Schurr et al, -c, 1999). Adenylates were measured by HPLC using a modification of the method of Teerlink et al (1993). The buffer concentration was reduced to 50 mM and its pH to 5.0.…”

Reversal of Thrombin-Induced Deactivation of CD39/ATPDase in Endothelial Cells by HMG-CoA Reductase Inhibition

“…Signals were detected with a UVvisible detector (HP series 1100, Hewlett-Packard). The chromatographic conditions were as follows: isocratic elution at room temperature with 0.1 mol/L NaH 2 PO 4 (pH 6.0 NaOH)/methanol (96%/ 4%); flow rate, 0.7 mL/min; pressure, Ϸ122 bar; UV-visible detector wavelength, 254 nm; injection volume, 30 L. 16 …”