Simultaneous determination of eleven antiepileptic compounds in serum by high-performance liquid chromatography

Wad, Nils

doi:10.1016/s0378-4347(00)83320-4

Cited by 66 publications

(20 citation statements)

References 4 publications

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“…In den achtziger Jahren hatte sich die HPLC mit UV-Detektion mehr und mehr als Methode für das TDM von AED etabliert. Es zeigte sich, dass die HPLC eine sehr leistungsfähige Analysenmethode ist, besonders, wenn es um das TDM bei Mehrfachmedikationen geht [3].…”

Section: Zeitgeschichtliche Entwicklungunclassified

“…Mit Beginn der achtziger Jahre wurde die HPLC für das TDM mehr und mehr zur Routinemethode [2,3], die im Gegensatz zu den Enzymimmunoassays immer weiter ausbaubar war. Auf neue Anforderungen beim TDM von AED konnte flexibel reagiert werden, weil eine Vielzahl von Möglichkeiten mit der HPLC umsetzbar war und es v. a auf das Geschick des Analytikers ankam, die entsprechenden Methoden zu entwickeln.…”

Section: Introductionunclassified

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Therapeutisches Drugmonitoring von Antiepileptika

Jürges

Steinhoff

2011

Z. Epileptol.

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Section: Zeitgeschichtliche Entwicklungunclassified

Section: Introductionunclassified

Therapeutisches Drugmonitoring von Antiepileptika

Jürges

Steinhoff

2011

Z. Epileptol.

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“…This simple approach for quantitation provided data which were equivalent to those obtained with linear calibration graphs constructed with 4 data points. Extraction was carried out according to the two methods of Wad (phenobarbital [15] and ethosuximide [16]). After evaporation of the organic solvent using a vortex evaporator, the residues were dissolved in 400 pL of running buffer prior to analysis.…”

Section: Effect Of Voltage and Injection Timementioning

confidence: 99%

Factors affecting the determination of drugs and endogenous low molecular mass compounds in human serum by micellar electrokinetic capillary chromatography with direct sample injection

Schmutz

Thormann

1994

Electrophoresis

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Factors influencing the establishment of an analytical window in front of the solubilized proteins in micellar electrokinetic capillary chromatography (MECC) with direct serum injection (DSI) are discussed. Both drugs and endogenous low molecular mass compounds eluting within the analytical window are identified concurrently by multi-wavelength absorption detection. Variables such as the concentration of the micelle forming substance, ionic strength, applied voltage, initial sample zone length, capillary length, selected buffer additives, insufficient renewal of the buffer in the anodic buffer vial and sample matrix are shown to impact MECC of endogenous compounds and model drugs, such as antiepileptics. For two drugs eluting within the analytical window, phenobarbital and ethosuximide, serum levels determined by DSI with external calibration are shown to compare well with levels obtained after liquid-liquid extraction and internal calibration (use of an internal standard). In addition, reproducibility of both assays is excellent. The limit of employing DSI is demonstrated with the determination of the hydrophobic drug phenytoin. Using an automated, commercial instrument and naproxen as model drug, high-speed MECC separations of high reproducibility and with a throughput of 12-15 samples per h are presented.

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“…For the simultaneous analysis of AEDs in human serum or plasma by high performance liquid chromatography (HPLC) several methods are reported [9][10][11][12][13][14][15]. However, these methods only include analysis of a number of classical AEDs and/or use a liquid-liquid extraction method, which is difficult to automatize.…”

Section: Introductionmentioning

confidence: 99%