Simultaneous Determination of Five Alkaloids by HPLC-MS/MS Combined With Micro-SPE in Rat Plasma and Its Application to Pharmacokinetics After Oral Administration of Lotus Leaf Extract

Zou, Shuhan; Ge, Yuanyuan; Chen, Xuanhao; Li, Jin; Yang, Xuejing; Wang, Hui; Gao, Xiumei; Chang, Yan‐xu

doi:10.3389/fphar.2019.01252

Cited by 18 publications

(11 citation statements)

References 24 publications

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“…The present study analysed the principal components of the lotus six parts by UPLC-QToF-MS. Combined the related literatures [ 6 , 32 , 33 ] with our previous experiments, heat reflux extraction with 80% ethanol was chosen for the samples extraction. And the detecting conditions, mobile phase elution solutions of UPLC, positive and negative ion detection modes of QToF-MS, were optimized by quality control samples.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Chemical Constituents in Different Parts of Lotus by UPLC and QToF-MS

Pei

Gui

et al. 2021

Molecules

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Six parts of lotus (seeds, leaves, plumule, stamens, receptacles and rhizome nodes) are herbal medicines that are listed in the Chinese Pharmacopoeia. Their indications and functions have been confirmed by a long history of clinical practice. To fully understand the material basis of clinical applications, UPLC-QToF-MS combined with the UNIFI platform and multivariate statistical analysis was used in this study. As a result, a total of 171 compounds were detected and characterized from the six parts, and 23 robust biomarkers were discovered. The method can be used as a standard protocol for the direct identification and prediction of the six parts of lotus. Meanwhile, these discoveries are valuable for improving the quality control method of herbal medicines. Most importantly, this was the first time that alkaloids were detected in the stamen, and terpenoids were detected in the cored seed. The stamen is a noteworthy part because it contains the greatest diversity of flavonoids and terpenoids, but research on the stamen is rather limited.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Chemical Constituents in Different Parts of Lotus by UPLC and QToF-MS

Pei

Gui

et al. 2021

Molecules

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“…The developed UPLC-MS/MS was used for the subsequent quantification of all Gelsemium alkaloids, which has a faster analysis time than traditional HPLC [ 35 , 36 ] or LC-MS [ 37 – 39 ] and greatly enhances the signal intensity. It only takes five minutes to analyze a plasma sample, which can save significant time when analyzing hundreds of samples.…”

Section: Discussionmentioning

confidence: 99%

Toxicokinetics of 11 Gelsemium Alkaloids in Rats by UPLC-MS/MS

Shen

Wang

et al. 2020

BioMed Research International

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Gelsemium elegans (Gardn. & Champ.) Benth. is a plant belonging to the genus Gelsemium (family Gelsemiaceae), and its main components are alkaloids. It is a Chinese traditional medicinal plant and notoriously known as a highly toxic medicine. However, a method has not yet been found for the simultaneous detection of 11 Gelsemium alkaloids in rat plasma, and the toxicokinetics of 11 Gelsemium alkaloids after intravenous administration has not been reported. In this work, we have developed a sensitive and rapid method of ultraperformance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) for the detection of 11 Gelsemium alkaloids in rat plasma. The toxicokinetic behavior was also investigated, so as to provide a reference of the scientific properties of Gelsemium elegans and improve the efficacy and safety of drugs. Sixty-six Sprague-Dawley rats were randomly divided into 11 groups, six rats in each group. Each group was intravenously given one alkaloid (0.1 mg/kg), respectively. A Waters UPLC BEH C18 column (50 mm×2.1 mm, 1.7 μm) was used for chromatographic separation. Methanol and water (containing 0.1% formic acid) were used for the mobile phase with gradient elution. Multiple reactions were monitored, and positive electrospray ionization was used for quantitative analysis. The precision was less than 16%, and the accuracy was between 86.9% and 113.2%. The extraction efficiency was better than 75.8%, and the matrix effects ranged from 88.5% to 107.8%. The calibration curves were in the range of 0.1–200 ng/mL, with a correlation coefficient (R2) greater than 0.995. The UPLC-MS/MS method was successfully applied to the toxicokinetics of 11 Gelsemium alkaloids in rats after intravenous administration (0.1 mg/kg for each alkaloid). The results of the toxicokinetics provide a basis for the pharmacology and toxicology of Gelsemium alkaloids and scientific evidence for the clinical use of Gelsemium alkaloids.

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“…In addition to precipitation protein methods and liquid-liquid extraction, many studies to measure natural compounds in biological matrix use the Solid Phase Extraction method. One of them is in the pharmacokinetic determination of five types of alkaloids (nunciferine, O-nornuciferin, liriodenine, armepavine, and pronuciferine) in Lotus Leaf Extract [62] samples of 100 μl and standard internal solutions at vortex were then extracted using SPE columns (Cleanert PEP-2, 40-60 μm, 60 Å, 10 mg /1 ml; Phenomenex, Torrance, CA, USA). In the first stage, the SPE column is poured with 50% methanol solution in water and 0.1% formic acid.…”

Section: Sample Preparation For Natural Productmentioning

confidence: 99%

“…The third step was carried out elution with acetonitrile solution after adding a plasma sample. The last step of the eluent is centrifuged and the solution obtained is injected or continued to the analysis stage [62] n the SPE method, the selection of sorbents is one of the key factors of extraction ability because physicochemical properties affect the absorption capacity for different types of sorbents. The SPE method is used to extract anthocyanin in plasma to measure bioavailability.…”

Section: Sample Preparation For Natural Productmentioning

confidence: 99%

Sample Preparation and Bioanalysis Validation for Natural Product Sample

2021

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Bioanalysis study and its validation are important for the application in natural drug discovery and preparation for natural medicine products with safe drug development approaches. Bioanalysis data for natural products can provide quantitative information on active compounds from natural products for pharmacokinetics, pharmacodynamics, bioavailability, bioequivalence, and toxicokinetics. Bioanalysis validation provides useful guidance for obtaining accurate data on drug discovery, preclinical and clinical testing of natural drug product development, and to ensure the methods used are suitable for application in testing the samples. In general, sample preparation and validation of the bioanalysis method are part of the pharmacokinetic characterization of a chemical compound from the discovery to the development stage of natural material products. Therefore, this paper aims to discuss how to prepare samples and validate bioanalysis for natural products.

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Simultaneous Determination of Five Alkaloids by HPLC-MS/MS Combined With Micro-SPE in Rat Plasma and Its Application to Pharmacokinetics After Oral Administration of Lotus Leaf Extract

Cited by 18 publications

References 24 publications

Comparative Analysis of Chemical Constituents in Different Parts of Lotus by UPLC and QToF-MS

Comparative Analysis of Chemical Constituents in Different Parts of Lotus by UPLC and QToF-MS

Toxicokinetics of 11 Gelsemium Alkaloids in Rats by UPLC-MS/MS

Sample Preparation and Bioanalysis Validation for Natural Product Sample

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