Simultaneous Determination of Five Coccidiostats in Veterinary Powders, Feed Premixes, and Baby Food by Micellar Electrokinetic Chromatography: Application to Chicken Tissues and Liver

Belal, F.; El-Razeq, Sawsan A. Abd; Fouad, Mostafa A.; Zayed, Sahar; Fouad, Fatma A.

doi:10.1007/s12161-018-1330-y

Cited by 17 publications

(21 citation statements)

References 29 publications

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“…To date, the use of MEKC has been reported for detecting Q [ 159 ], LA [ 160 ], COC [ 161 ], AP [ 162 ], SA [ 163 ] and PCN [ 164 ] residues in poultry tissues, milk, and eggs. Kowalski et al [ 160 ] established a MEKC method combined with a UVD for the detection of lincomycin and clindamycin in poultry tissue samples.…”

Section: Analytical Methods For Detectionmentioning

confidence: 99%

Veterinary Drug Residues in Animal-Derived Foods: Sample Preparation and Analytical Methods

Wang

Xie

Lee

2021

Foods

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Veterinary drugs are used to treat livestock and aquatic diseases and thus are introduced into animal-derived foods, endangering consumer health and safety. Antibiotic resistance is rapidly becoming a major worldwide problem, and there has been a steady increase in the number of pathogens that show multi-drug resistance. Illegal and excessive use of veterinary drugs in animals and aquaculture has serious adverse effects on humans and on all other environmental organisms. It is necessary to develop simple extraction methods and fast analytical methods to effectively detect veterinary drug residues in animal-derived foods. This review summarizes the application of various sample extraction techniques and detection and quantification methods for veterinary drug residues reported in the last decade (2010-2020). This review compares the advantages and disadvantages of various extraction techniques and detection methods and describes advanced methods, such as those that use electrochemical biosensors, piezoelectric biosensors, optical biosensors, and molecularly imprinted polymer biosensors. Finally, the future prospects and trends related to extraction methods, detection methods and advanced methods for the analysis of veterinary drug residues in animal-derived foods are summarized.

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Section: Analytical Methods For Detectionmentioning

confidence: 99%

Veterinary Drug Residues in Animal-Derived Foods: Sample Preparation and Analytical Methods

Wang

Xie

Lee

2021

Foods

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“…However, due to the low UV and FLD absorbance of polyether antibiotics, it is often necessary for them to be derivatized for detection, which is not only time-consuming but the sample pretreatment becomes more complex. In addition, various analytical methods have been used to measure anticoccidial drugs in eggs, chicken tissue, milk, duck muscle, pork, beef, and other animal foods, including competitive enzyme-linked immunosorbent assays (cELISA) [13], micellar electrokinetic chromatography (MEKC) [14], flow cytometry-based immunoassays (FCIA) [15], hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) [16], and liquid chromatography-tandem mass spectrometry (LC-MS/MS) . Compared with other detection methods, the LC-MS/MS method has the advantages of qualitative and quantitative accuracy, it is fast and does not require analyte derivatization, and its sensitivity could allow for the detection of low-concentration substances.…”

Section: Introductionmentioning

confidence: 99%

Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

et al. 2020

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A method for the simultaneous determination of robenidine, halofuginone, lasalocid, monensin, nigericin, salinomycin, narasin, and maduramicin residues in eggs by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed. The sample preparation method used a combination of liquid–liquid extraction (LLE) and solid-phase extraction (SPE) technology to extract and purify these target compounds from eggs. The target compounds were separated by gradient elution using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). Tandem mass spectrometry was used to quantitatively and qualitatively analyze the target compounds via electrospray ionization (ESI+) and multiple reaction monitoring mode. The HPLC–MS/MS and UPLC–MS/MS methods were validated according to the requirements defined by the European Union and the Food and Drug Administration. The limits of detection and limits of quantification of the eight coccidiostats in eggs were 0.23–0.52 µg/kg and 0.82–1.73 µg/kg for HPLC–MS/MS, and 0.16-0.42 µg/kg and 0.81-1.25 µg/kg for UPLC–MS/MS, respectively. The eggs were spiked with four concentrations of the eight coccidiostats, and the HPLC–MS/MS and UPLC–MS/MS average recoveries were all higher than 71.69% and 72.26%, respectively. Compared with the HPLC–MS/MS method, utilizing UPLC–MS/MS had the advantages of low reagent consumption, a short detection time, and high recovery and precision. Finally, the HPLC–MS/MS and UPLC–MS/MS methods were successfully applied to detect eight coccidiostats in 40 eggs.

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“…These methods include LC‐UV or fluorescence detection and LC‐MS . A micellar electrokinetic chromatographic method has been developed for the simultaneous determination of five coccidiostats including AMP and EPB in veterinary and food products . Additionally, TLC‐densitometric method has been reported for determination of AMP and EPB in veterinary preparation .…”

Section: Introductionmentioning

confidence: 99%

“…Coccidian protozoa, primarily Eimeria, cause economically significant infections in domesticated animals. The coccidiostat drugs amprolium hydrochloride (AMP) and ethopabate (EPB) are widely simultaneous determination of five coccidiostats including AMP and EPB in veterinary and food products [8]. Additionally, TLC-densitometric method has been reported for determination of AMP and EPB in veterinary preparation [9].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive stability‐indicating high‐performance liquid chromatography coupled with diode array detection method for simultaneous determination of amprolium hydrochloride and ethopabate in powder dosage form for veterinary use

Baker

El-Kafrawy

Abdel-Khalek

et al. 2019

J of Separation Science

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This research deals with the development of a stability‐indicating high‐performance liquid chromatography method for simultaneous determination of amprolium hydrochloride and ethopabate. To the best of our knowledge, no comprehensive stability‐indicating method has been reported for analysis of this mixture. Separation was achieved using Kromasil cyano column with gradient elution of the mobile phase composed of sodium hexane sulfonate solution and methanol. Quantification was based on measuring peak areas at 266 nm. Amprolium and ethopabate peaks eluted at retention times 10.42 and 18.53 min, respectively. The proposed procedure was validated with respect to system suitability, linearity, ranges, precision, accuracy, specificity, robustness, detection, and quantification limits. Linearity ranges for amprolium and ethopabate were 1.5–240 and 1–160 μg/mL, respectively. Analytes were subjected to stress conditions of hydrolysis, oxidation and thermal degradation. The proposed method enabled resolution of drugs from their forced‐degradation products and amprolium related substance (2‐picoline). Moreover, specificity was verified by resolution of the analytes from about 22 drugs used in antimicrobial veterinary products. The validated method was successfully applied to assay of the combined veterinary powder dosage form, additionally it was implemented in the accelerated stability study of the dosage form when stored for six months at 40°C and 75% relative humidity.

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Simultaneous Determination of Five Coccidiostats in Veterinary Powders, Feed Premixes, and Baby Food by Micellar Electrokinetic Chromatography: Application to Chicken Tissues and Liver

Cited by 17 publications

References 29 publications

Veterinary Drug Residues in Animal-Derived Foods: Sample Preparation and Analytical Methods

Veterinary Drug Residues in Animal-Derived Foods: Sample Preparation and Analytical Methods

Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Comprehensive stability‐indicating high‐performance liquid chromatography coupled with diode array detection method for simultaneous determination of amprolium hydrochloride and ethopabate in powder dosage form for veterinary use

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