Simultaneous determination of five marker compounds in Xuanfu Daizhe Tang by high-performance liquid chromatography coupled with diode array detection for quality control

Qin, Kunming; Wang, Binbin; Cai, Hao; Li, Weidong; Yao, Zhongqing; Zhang, Xingde; Lu, Tu-Lin; Cai, Baochang

doi:10.4103/0973-1296.103647

Cited by 9 publications

(3 citation statements)

References 14 publications

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“…Intra- and interday repeatability was determined on six replicates within 1 day and 5 consecutive days, respectively. [ 13 ]…”

Section: Methodsmentioning

confidence: 99%

Simultaneous quantification of nine major active components in traditional Chinese prescription Mahuang decoction and the influence of herbal compatibility on their contents

Zhu¹,

Zhang²,

Ge³

et al. 2014

Phcog Mag

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Background:Mahuang decoction (MHD), a famous classic traditional Chinese formula, has been extensively applied for treating cold, influenza, asthma, acute bronchitis, and other pulmonary diseases. However, the interaction among four drugs of MHD has not been clearly deciphered from the aspect of molecular composition.Objective:To assess the quality of MHD and explore the interplay among different prescription drugs.Materials and Methods:A reversed-phase high performance liquid chromatography (RP-HPLC) coupled with diode array detector (DAD) method for the simultaneous separation and determination of nine bioactive components was developed. A somatomedin A (SMA)-phenyl column (4.6 mm × 250 mm, 5 μm) was eluted by a gradient mobile phase contained acetonitrile and 0.05% formic acid-0.05% triethylamine aqueous solution. Four detection wavelengths (210, 252, 278, and 291 nm) were utilized for the quantitative analysis due to the different ultraviolet (UV) spectra of these compounds.Results:Satisfactory separation was obtained for all the components, and the assay was fully validated in respects of linearity, precision, stability, and accuracy. It was found that the calibration curves for all analytes showed good linearity (R2≥ 0.9991) within the test ranges. The relative standard deviations (RSDs) for intra- and interday repeatability were not more than 1.70 and 2.66%, respectively. The spike recoveries of nine components varied from 97.50 ± 1.69 to 99.27 ± 1.37%.Conclusion:The established method was successfully applied to analyze nine active compounds in decoction samples of various drug compatibilities of MHD. The variations of contents were obvious for different combinations, which hinted the mutual promotion or inhibition of componential dissolution among four herbs of MHD.

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“…Intra- and interday repeatability was determined on six replicates within 1 day and 5 consecutive days, respectively. [ 13 ]…”

Section: Methodsmentioning

confidence: 99%

Simultaneous quantification of nine major active components in traditional Chinese prescription Mahuang decoction and the influence of herbal compatibility on their contents

Zhu¹,

Zhang²,

Ge³

et al. 2014

Phcog Mag

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“…Xuanfu Daizhe (XFDZ) decoction is a traditional Chinese medicine that is composed of seven medicinal herbs: Inulae Flos(xuanfuhua), Haematitum(daizheshi), Pinelliae Rhizoma Praeparatum(qingbanxia), Ginseng Radix Et Rhizoma(renshen), Roast Radix Glycyrrhizae(zhigancao), Jujubae Fructus (dazao) and Zingiberis Rhizoma Recens (shengjiang) [10]. XFDZ have been recorded in the 2020 edition of Chinese Pharmacopoeia (Part I) [11].…”

Section: Introductionmentioning

confidence: 99%

“…XFDZ have been recorded in the 2020 edition of Chinese Pharmacopoeia (Part I) [11]. In China, this famous formula has been widely applied for the treatment of digestive system diseases, such as chronic gastritis, stomach neurosis and re ux esophagitis [10,[12][13][14]. However, there is no su cient experimental evidence for the effects of XFDZ on RE.…”

Section: Introductionmentioning

confidence: 99%

Xuanfudaizhe Decoction Alleviate Reflux Esophagitis through Inhibition of NLRP3/Caspase-1 Pathway

liu

Wang

Dai

et al. 2023

Preprint

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Background: Reflux esophagitis (RE) is a clinically common digestive disease, and the main pathological manifestation of RE is esophageal mucosa inflammatory damage. Xuanfu Daizhe (XFDZ) decoction is a traditional Chinese herbal compound that is famous for RE treatment, but the pharmacological and molecular mechanism of XFDZ remains largely unknown. Methods: The active ingredients of XFDZ were detected using the ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS). The rat model of RE was established with pylorus clamp and 2/3 fundus of stomach ligated. XFDZ (8.55 g/kg) and Omeprazole + Mosapride (1.35 g/kg) were orally administered for 14 days. Pathology of esophageal mucosal inflammation was evaluated under microscopy by hematoxylin-eosin (HE) staining. In vitro, by inducing cellular inflammatory response with glycocholic and taurocholic acid mixture (PH 4.7 acid medium added 500 μmol/L concentration of mixed bile acids) in human esophageal epithelial cells(HEEC). The mitochondrial membrane potential was detected using JC-1 fluorescence mitochondrial imaging. The mtDNA copy number was determined via quantitative real-time polymerase chain reaction (qRT-PCR). Fluorescent probe DCFH-DA was used to detect ROS. The relative fluorescence expression of NLRP3 inflammasome was determined by high-intension cell imaging and quantitative fluorescence techniques. ELISA was used to detect and quantify inflammatory cytokines related to the NLRP3 inflammasome. Western blot analysis was performed to investigate proteins that are associated with the NLRP3 inflammasome. Results: Twenty chemical components such as alkaloids and flavonoids were identified in the analysis of XFDZ, which may be the material basis for XFDZ to exert its effect. XFDZ can significantly reduce the contents of Caspase-1, IL-1β and IL-18 in serum of rats, and down-regulate the protein expression levels of NLRP3, Caspase-1, IL-1β and IL-18 in esophageal tissue. The simulated reflux could decrease the membrane potential, increase the ROS production, decrease the relative expression of mtDNA and activate the NLRP3/Caspase-1 signaling pathway in vitro. XFDZ had no obvious protective effect on the membrane potential and mtDNA, but could inhibit ROS production and the activation of NLRP3/Caspase-1 signaling pathway. Conclusion: We concluded that XFDZ could reduce the inflammatory damage in RE by inhibiting NLRP3/Caspase-1 signaling pathway both in vitroand in vivo, indicating the capability of XFDZ as a promising drug for the treatment of RE.

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Development of validated determination of the eleven marker compounds in Gyejibokryeong-hwan for the quality assessment using HPLC analysis

Seo

Shin

2014

Arch. Pharm. Res.

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This study was designed for quality assessment of Gyejibokryeong-hwan (GBH) preparations using the simultaneous determination of 11 marker compounds such as gallic acid, oxypaeoniflorin, amygdalin, albiflorin, paeoniflorin, benzoic acid, coumarin, cinnamic acid, benzoylpaeoniflorin, cinnamaldehyde, and paeonol using HPLC-PDA and chemometric analysis. The correlation coefficient of linear regression was >0.9993. The LOD and LOQ values were in the ranges of 0.02-2.11 and 0.06-7.34 μg/mL, respectively. The intra-day and inter-day precision was 0.07-4.76 and 0.04-5.08 %, and the recovery range was 92.19-103.24 %, with RSD values <4.5 %. The repeatability of the 11 marker compounds was <2.5 % of the RSD values. The results of quantification indicated that there was much variation in the content of the 11 marker compounds among the GBH preparations. Amygdalin and paeoniflorin were detected in high quantities in all GBH samples, whereas some compounds such as cinnamaldehyde and paeonol were not detected in GBH samples from the Korean manufacturers. The content of marker compounds in the laboratory GBH water extract was different to the commercial preparations. Results of analyses carried out with the method indicate the need for quality control of GBH preparations.

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Simultaneous determination of five marker compounds in Xuanfu Daizhe Tang by high-performance liquid chromatography coupled with diode array detection for quality control

Cited by 9 publications

References 14 publications

Simultaneous quantification of nine major active components in traditional Chinese prescription Mahuang decoction and the influence of herbal compatibility on their contents

Simultaneous quantification of nine major active components in traditional Chinese prescription Mahuang decoction and the influence of herbal compatibility on their contents

Xuanfudaizhe Decoction Alleviate Reflux Esophagitis through Inhibition of NLRP3/Caspase-1 Pathway

Development of validated determination of the eleven marker compounds in Gyejibokryeong-hwan for the quality assessment using HPLC analysis

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