Simultaneous Determination of Four Active Components in Tobacco Wastes by LC

Abstract

A liquid chromatographic method was developed for the simultaneous quantification of four major active components in tobacco (Nicotiana tobaccum L.) wastes. Samples were extracted with 70% v/v aqueous methanol, four compounds including chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid and caffeic acid were identified and determined by using LC coupled to electrospray tandem mass spectrometry and LC-UV method, respectively. Separation in LC-UV was on an Alltima C 18 column (250 mm 9 4.6 mm i.d.; 5 lm) with a mobile phase consisting acetonitrile: ammonium acetate buffer (pH 4.5) (5:95 v/v), at a flow rate of 1.0 mL min-1 , detected at 327 nm. Four regression equations showed good linear relationships (r 2 > 0.999) between the peak area of each marker and concentration. The method has good repeatability and precision, the intra-day and inter-day RSD for both retention time and peak area was less than 1.0%. The recoveries, measured at three concentration levels, varied from 96.33 to 101.10%. The LOD (S/N = 3) and LOQ (S/N = 6) were less than 0.010 and 0.795 lgÁmL-1 , respectively. This assay was successfully applied to the determination of four active compounds in ten samples. The results indicated that the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for various of tobacco wastes.

Discrimination and classification of tobacco wastes by identification and quantification of polyphenols with LC-MS/MS

“…The objective of this work was the evaluation of tobacco waste as a potential ethnopharmacological resource for the production of chlorogenic acids and rutin, in order to develop a new bioprocess for the synthetic utilization of tobacco waste. 27 Processing tobacco wastes could solve the environmental problems derived from the disposal of large amounts of tobacco waste. Moreover, obtaining stabilized tobacco wastes as natural ethnopharmacological materials could be advantageous to the traditional Chinese medicine (TCM) industry in China.…”

Isolation of mono‐caffeoylquinic acids from tobacco waste using continuous resin‐based pre‐separation and preparative HPLC

“…In our previous study, acetonitrile–ammonium acetate buffer (pH 4.5) (5:95 v/v) was successfully developed as the eluting solvent system for the separation of mono‐CQAs on an analytical HPLC column []. However, this mobile phase could not be used for preparative HPLC, due to the expense of acetonitrile and the large volume of salts required.…”

“…However, the huge demand for these natural resources for the production of mono‐CQAs is a global challenge. Fortunately, the active compounds of tobacco ( Nicotiana tobaccum L.) wastes have attracted considerable attention as a good source of mono‐CQAs, with total content varying from 0.896 to 2.169% []. In China, 460 million kg of tobacco waste per year is generated at various stages of the post‐harvest processing and manufacture of tobacco [].…”

“…Fortunately, the active compounds of tobacco ( Nicotiana tobaccum L.) wastes have attracted considerable attention as a good source of mono‐CQAs, with total content varying from 0.896 to 2.169% []. In China, 460 million kg of tobacco waste per year is generated at various stages of the post‐harvest processing and manufacture of tobacco []. Therefore, the isolation of mono‐CQAs from tobacco waste is of great importance for its large‐scale application in the pharmaceutical industry.…”

“…Nowadays, the preparative separation and purification of mono‐CQAs usually focuses on 5‐CQA, meaning that less attention has been paid to 3‐CQA and 4‐CQA. Numerous papers have been published in qualitative and quantitative analysis of 5‐CQA in the extracts of plants by high‐performance liquid chromatography (HPLC) [], HPLC‐MS [], HPLC‐NMR (where NMR is nuclear magnetic resonance) [] and capillary electrophoresis []. Several reports are related to their extraction and isolation, including enrichment of phenolic compounds by pressurised liquid extraction [], preparation of total caffeoylquinic acids using accelerated solvent extraction [], enrichment of total mono‐CQAs by macroporous adsorption resin [], separation of 5‐CQA by centrifugal partition chromatography [], preparation of 5‐CQA by preparative HPLC [] and purification of dicaffeoylquinic acids (3,5‐O‐dicaffeoylquinic acid, 3,4‐O‐dicaffeoylquinic acid) and 3‐CQA by high‐speed counter‐current chromatography (HSCCC) [].…”