Simultaneous determination of four anthracyclines and three metabolites in human serum by liquid chromatography–electrospray mass spectrometry

Lachâtre, F; Marquet, Pierre; Ragot, Sylvie; Gaulier, Jean‐Michel; Cardot, Philippe; Dupuy, Jean-Louis

doi:10.1016/s0378-4347(99)00529-0

Cited by 97 publications

(82 citation statements)

References 19 publications

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“…One method could be applied to each of the four pairs (main compound and respective reduced metabolite) individually [68]. Four methods were developed that could determine simultaneously two or more main compounds alone [69] or together with their reduced metabolites [13,[70][71][72].…”

Section: Analytes and Concentrations Of Interestmentioning

confidence: 99%

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Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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Section: Analytes and Concentrations Of Interestmentioning

confidence: 99%

“…Besides silica based reversedphase C18 [46,47,56,60,66,67,70], C8 [72] and C2 [44,65] sorbents, also polymeric Oasis HLB [48,49,57,71] and MCX [50] sorbents have been employed. A Biotrap 500 MS online SPE column has also been used [69].…”

Section: Solid Phase Extractionmentioning

confidence: 99%

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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“…To date, a number of publications have been reported of the determination of DXR and its metabolite DXR-ol including capillary electrophoresis (CE) with UV detection [4] or laser-induced fluorescence (LIF) detection [5][6][7], radioimmunoassay [8], high performance liquid chromatography (HPLC) with ultraviolet (UV) detection [9][10], fluorescence detection [11][12][13][14][15][16][17][18][19][20][21], chemiluminescence detection [22], electrochemical detection [23] and mass spectrometric detection [25][26][27][28][29]. However, many of these methods lack the enough sensitivity and selectivity which enable the determination of DXR and DXR-ol at small plasma concentration while the high cost and the need for technical experience limits the use of the others.…”

Section: Doxorubicin (Dxr) Is a Cytotoxic Anthracycline Antibiotic Thmentioning

confidence: 99%

“…Some of them include time-consuming solid-phase extraction processes [23][24][25][26], or liquid-liquid extraction procedures [4,12,17,20,28]. The instability of DXR and DXR-ol in plasma also limits the utility of otherwise promising extraction strategies.…”

Section: Doxorubicin (Dxr) Is a Cytotoxic Anthracycline Antibiotic Thmentioning

confidence: 99%

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Ahmed

Kishikawa

Ohyama

et al. 2009

Talanta

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A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15 min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-L was sufficient. Besides, it can detect accurately the low plasma concentrations.The detection limits (signal-to-noise ratio = 3) were 4.5 and 3.8 fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7-102.4 % and the inter-and intra-assay RSD values in rat plasma were 2.5-8.9 %. The method has been successfully used to study pharmacokinetic profiles of DXR and DXR-ol in rats after a single dose of DXR.

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“…These include HPLC and capillary electrophoresis with either fluorescence or UV detection, as well as mass spectrometry and enzyme immunoassays [5][6][7]11]. The most widely used methodology for the quantification of DOX is HPLC with fluorescence detection.…”

Section: Introductionmentioning

confidence: 99%

Accelerator mass spectrometry allows for cellular quantification of doxorubicin at femtomolar concentrations

DeGregorio

Dingley

Wurz

et al. 2005

Cancer Chemother Pharmacol

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Accelerator mass spectrometry (AMS) is a highly sensitive analytical methodology used to quantify the content of radioisotopes, such as 14 C, in a sample. The primary goals of this work were to demonstrate the utility of AMS in determining cellular [ 14 C]doxorubicin (DOX) concentrations and to develop a sensitive assay that is superior to high performance liquid chromatography (HPLC) for the quantification of DOX at the tumor level. In order to validate the superior sensitivity of AMS versus HPLC with fluorescence detection, we performed three studies comparing the cellular accumulation of DOX: one in vitro cell line study, and two in vivo xenograft mouse studies. Using AMS, we quantified cellular DOX content up to 4 hours following in vitro exposure at concentrations ranging from 0.2 pg/ml (345 fM) to 2 Fg/ml (3.45The results of this study show that, compared to standard fluorescence-based HPLC, the AMS method was over five orders of magnitude more sensitive. Two in vivo studies compared the sensitivity of AMS to HPLC using a nude mouse xenograft model in which breast cancer cells were implanted subcutaneously. After sufficiently large tumors formed, DOX was administered intravenously at two dose levels. Additionally, we tested the AMS method in a nude mouse xenograft model of multidrug resistance (MDR) in which each mouse was implanted with both wild type and MDR+ cells on opposite flanks. The results of the second and third studies showed that DOX concentrations were significantly higher in the wild type tumors compared to the MDR+ tumors, consistent with the MDR model. The extreme sensitivity of AMS should facilitate similar studies in humans to establish target site drug delivery and to potentially determine the optimal treatment dose and regimen.3

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Simultaneous determination of four anthracyclines and three metabolites in human serum by liquid chromatography–electrospray mass spectrometry

Cited by 97 publications

References 19 publications

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Accelerator mass spectrometry allows for cellular quantification of doxorubicin at femtomolar concentrations

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