Simultaneous determination of free and conjugated bile acids in serum by cyclodextrin-modified micellar electrokinetic chromatography

Intrahepatic Cholestasis of Pregnancy: The Usefulness of Serum Bile Acid Profile for Diagnosis and Treatment

“…It is suggested that patients could be expectantly managed when TSBA levels remain below 40 µM and when pregnant women refer pruritus it should be surveilled with the aid of repeated determinations of serum bile acids. (Glantz et al, 2004) Recently, a subgroup of asymptomatic pregnant women with high levels of TSBA, and normal liver function tests but not showing pruritus has been classified as asymptomatic hypercholanaemic pregnants (AHP) (Castaño et al, 2006;Lunzer et al, 1986;Pascual et al, 2002;Tripodi et al, 2003). Women with AHP did not differ from normocholanemic women respect to clinical, biochemical or perinatal characteristics.…”

“…They have different physico-chemical properties according to the number, position and orientation of their hydroxylgroups, and by conjugation with glycine and taurine, glyco-and tauro-derivatives are produced to the liver (Lucangioli et al, 2001). Figure 1 presents the chemical structures of bile acids showing primary BA like cholic acid (CA) and chenodeoxycholic acid (CDCA) and secondary BA, deoxycholic acid (DCA), lithocholic acid (LCA) and ursodeoxycholic (UDCA) (Tripodi et al, 2003). Moreover, BA are acidic molecules with pka values of 1.5 for taurine derivatives, 4.5 for glycine derivatives and 6 for unconjugated BA.…”

“…The CE system is based on a micellar electrokinetic chromatography, as a mode of CE, with the use of sodium dodecyl sulfate as micellar agent and cyclodextrins (CD-MEKC) (Tripodi et al, 2003) using UV detection. The CD-MEKC system allowed the simultaneous and complete resolution of 15 BA (taurine, glycine and unconjugted BA) in serum sample in less than 12 min with good precision and accuracy using a simple sample preparation (Fig.…”

Chiral resolutions of (9‐anthryl)methoxyacetic acid and (9‐anthryl)hydroxyacetic acid by capillary electrophoresis

“…The BGE pH is a very important parameter when chiral CE analysis of organic acids use CDs or their derivatives as chiral selectors, 22 because the analytes, organic acids, possess ionizable carboxyl groups. The change of BGE pH influences the charge of analytes, thus, the electrostatic interactions of analyte and chiral selector, the electropho- retic motilities and the stability of analytes are also influenced.…”

Recent advances in micellar electrokinetic chromatography

“…Organic solvent additives can alter analyte selectivity, which may be beneficial in separating highly hydrophobic components. Reported solvent additives have included methanol [20,29,31,32,61,[71][72][73][74][75][76][77], ethanol [78][79][80], propyl alcohol [60,[81][82][83][84][85][86][87][88][89][90], acetonitrile [31,32,46,64,79,[91][92][93][94][95][96][97][98][99][100][101][102], or tetrahydrofuran [103,104]. Ionic liquids have also been used as a solvent additive [105].…”