Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC

Sultana, Najma; Shamim, Sana; Gul, Somia; Arayne, M. Saeed

doi:10.1590/s0100-40422010000700029

Cited by 6 publications

(2 citation statements)

References 9 publications

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“…In addition, the symmetry factor 1.29 was lower than the upper limit (1.5) according to ICH guidelines and consistent with previous results [31]. From the literature, there is no available data about the plate number of column in FUR quantitation by HPLC.…”

Section: Resultssupporting

confidence: 89%

Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

Youm

Youan

2013

Journal of Analytical Methods in Chemistry

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A simple, sensitive, and specific method for furosemide (FUR) analysis by reverse-phase-HPLC was developed using a Spherisorb C18 ODS 2 column. A chromatographic analysis was carried out using a mobile phase consisting of acetonitrile and 10 mM potassium phosphate buffer solution: 70 : 30 (v/v) at pH 3.85, at a flow rate of 1 mL·min−1. The UV-detection method was carried out at 233 nm at room temperature. Validation parameters including limit of detection (LOD), limit of quantitation (LOQ), linearity range, precision, accuracy, robustness, and specificity were investigated. Results indicated that the calibration curve was linear (r 2 = 0.9997) in the range of 5.2 to 25,000 ng·mL−1, with ε value equal to 3.74 × 104 L·M−1 ·cm−1. The LOD and LOQ were found to be 5.2 and 15.8 ng·mL−1, respectively. The developed method was found to be accurate (RSD less than 2%), precise, and specific with an intraday and interday RSD range of 1.233–1.509 and 1.615 to 1.963%. The stability of native FUR has also been performed in simulated perilymph and endolymph media (with respective potency in each medium of 99.8 ± 2.3% and 96.68 ± 0.7%, n = 3) after 6 hours. This method may be routinely used for the quantitative analysis of FUR from nanocarriers, USP tablets and release media related to hearing research

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Section: Resultssupporting

confidence: 89%

Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

Youm

Youan

2013

Journal of Analytical Methods in Chemistry

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“…There have been few reported analytical methods for the estimation of GFX in pharmaceutical preparations or biological fluids namely, high-performance liquid chromatography (2)(3)(4)(5)(6)(7)(8), high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) (9,10), capillary electrophoresis (11,12), voltammetry (13), chemiluminescence (14), spectrophotometry (15)(16)(17)(18)(19)(20) and spectrofluorimetry (21).…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric analysis of gemifloxacin mesylate in pharmaceutical formulations

et al. 2013

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A simple, rapid and highly sensitive spectrofluorimetric method was developed for determination of gemifloxacin mesylate (GFX) in tablets. The method is based on measuring the native fluorescence of GFX in isopropanol at 400 nm after excitation at 272 nm. The fluorescence-concentration plot was rectilinear over the range of 0.01-0.50 µg/mL with a lower detection limit of 1.19 ng/mL and quantification limit of 3.6 ng/mL. The method was fully validated and successfully applied to the determination of GFX tablets with an average percentage recovery of 99.65 ± 0.532. The method was extended to the stability study of GFX. The drug was exposed to acidic, alkaline, oxidative and photolytic degradation according to International Conference on Harmonization guidelines. The rate of GFX degradation was found at its highest in acidic conditions, and in its lowest in the neutral one. However, it was stable under dry heat and photolytic degradation conditions.

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Validated Method for the Simultaneous Determination of Gemifloxacin and H₂‐receptor Antagonists in Bulk, Pharmaceutical Formulations and Human Serum by RP‐HPLC; In‐vitro Applications

Sultana

Arayne

Shamim

et al. 2011

J Chinese Chemical Soc

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Simple, isocratic and rapid RP-HPLC method has been developed for the simultaneous analysis of gemifloxacin and H 2 -receptor antagonists i.e. Cimetidine, Famotidine and Ranitidine, in bulk, pharmaceutical formulation and human serum. Separation was achieved on the RP-Mediterranea column [C18 (250´4.6 mm, 5 µm)] at ambient temperature using mobile phase consisting of acetonitrile: methanol: water (20:28:52 v/v/v pH 2.8 adjusted by phosphoric acid). Flow rate was 1.0 mL/min with an average operating pressure of 180 kg/cm 2 . Gatifloxacin (GATI) was used as an internal standard (IS). Quantitation was achieved with UV detection at 221, 256 and 267 nm, respectively. Linear calibration curves, at concentration ranges of 0.05-37.5 mgmL -1 with a correlation coefficient of ±0.9994. The detection and quantification limits were in the ranges of 0.023-0.250 µgmL -1 and 0.071-0.756 µgmL -1 , respectively. Friedman's and Student's t-test were applied to correlate these results. Method was validated in terms of selectivity, linearity, precision, robustness, recovery, limits of detection and quantitation and is applicable to the routine analysis of GFX and H 2 -receptor antagonists, alone or in combination.

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Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC

Cited by 6 publications

References 9 publications

Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

Spectrofluorimetric analysis of gemifloxacin mesylate in pharmaceutical formulations

Validated Method for the Simultaneous Determination of Gemifloxacin and H₂‐receptor Antagonists in Bulk, Pharmaceutical Formulations and Human Serum by RP‐HPLC; In‐vitro Applications

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Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC

Cited by 6 publications

References 9 publications

Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

Spectrofluorimetric analysis of gemifloxacin mesylate in pharmaceutical formulations

Validated Method for the Simultaneous Determination of Gemifloxacin and H2‐receptor Antagonists in Bulk, Pharmaceutical Formulations and Human Serum by RP‐HPLC; In‐vitro Applications

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Validated Method for the Simultaneous Determination of Gemifloxacin and H₂‐receptor Antagonists in Bulk, Pharmaceutical Formulations and Human Serum by RP‐HPLC; In‐vitro Applications