Simultaneous determination of methamphetamine and its metabolite, amphetamine, in urine using a high performance liquid chromatography column-switching method

Accelerated quantification of amphetamine enantiomers in human urine using chiral liquid chromatography and on-line column-switching coupled with tandem mass spectrometry

“…Five samples collected from suspected MA abusers contained only S-AM, with concentrations ranging from 0.76 to 7.94 mg/L. The total concentrations of the AM enantiomers were in the range of those measured by Kumihashi et al [38] in urine samples from MA abusers. As reported for AM, the S-enantiomer of MA was found to be metabolized at a greater rate than the R-enantiomer [24].…”

Development and validation of a high-throughput method for the quantitative analysis of d-amphetamine in rat blood using liquid chromatography/MS3 on a hybrid triple quadrupole-linear ion trap mass spectrometer and its application to a pharmacokinetic study

“…In the past, numerous methods have been developed for the determination of d-amphetamine in biological samples including those using capillary electrophoresis with UV detection (CE/UV) [5], and those using a liquid chromatography (LC) with a UV detector [6][7][8][9][10], or a fluorescence detector [11]. One of the major limitations of these methods is a poor selectivity, which de facto limits the sensitivity and requires complicated sample preparations to remove matrix interferences and long separation times up to 30 min to resolve these from the analyte.…”

“…Even with unit resolution on triple quadrupole systems, in the majority of cases, single chromatographic peaks will be observed. Hence, several LC-MS/MS applications for analysis of d-amphetamines have been developed recently for the determination of d-amphetamine in urine, plasma and in serum samples [15][16][17]. Despite the highest sensitivity achieved with this technique, however, in some cases, the presence of endogenous species in biological extracts can still lead to interferences, even in MS/MS mode.…”

Simultaneous determination of 11 designated hallucinogenic phenethylamines by ultra-fast liquid chromatography with fluorescence detection

“…Furthermore, GC-MS [15][16][17][18] and LC-MS [19][20][21][22][23][24][25][26][27][28] are used for the preliminary study and mass screening to discriminate them from other hallucinogens. Although the individual determination of several phenethylamines have been performed [29,30], the simultaneous determination method of various phenethylamine analogues in a short run time, which is simple, sensitive, selective and quantitative, has not yet been developed.…”