Simultaneous determination of molecular weights and contents of water-soluble polysaccharides and their fractions from Lycium barbarum collected in China

Wu, Ding‐Tao; Lam, Shing Chung; Cheong, Kit-Leong; Wei, Feng; Lin, Peng-Cheng; Long, Zerong; Lv, Xiao-Jie; Zhao, Jing; Ma, Shuang‐Cheng; Li, Shaoping

doi:10.1016/j.jpba.2016.07.005

Cited by 65 publications

(29 citation statements)

References 29 publications

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“…Lycium barbarum L . (LBL) is a deciduous woody perennial plant primarily in the Ningxia Hui Autonomous Region (Ningxia, China) ( 27 ). Polysaccharides of LBL (LBLP) are the main bioactive components ( 28 , 29 ).…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Polysaccharide IV from Lycium barbarum L. Improves Lipid Profiles of Gestational Diabetes Mellitus of Pregnancy by Upregulating ABCA1 and Downregulating Sterol Regulatory Element-Binding Transcription 1 via miR-33

Yang

Fan

et al. 2018

Front. Endocrinol.

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Lycium barbarum L. (LBL) has beneficial effects on gestational diabetes mellitus (GDM) but the related mechanism remains unclear. Polysaccharides of LBL (LBLP) are the main bioactive components of LBL. miR-33, ATP-binding cassette transporter A1 (ABCA1) and sterol regulatory element-binding transcription 1 (SREBF1) affect lipid profiles, which are associated with GDM risk. LBLP may exert protective against GDM by affecting these molecules. Four LBLP fractions: LBLP-I, LBLP-II, LBLP-III, and LBLP-IV were isolated from LBL and further purified by using DEAE-Sephadex column. The effects of purified each fraction on pancreatic beta cells were comparatively evaluated. A total of 158 GDM patients were recruited and randomly divided into LBL group (LG) and placebo group (CG). miR-33 levels, lipid profiles, insulin resistance and secretory functions were measured. The association between serum miR-33 levels and lipid profiles were evaluated by using Spearman’s rank-order correlation test. After 4-week therapy, LBL reduced miR-33 level, insulin resistance and increased insulin secretion of GDM patients. LBL increased the levels of ABCA1, high-density lipoprotein cholesterol (HDL-C) and reduced miR-33, SREBF1, low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), triglyceride (TG), and malondialdehyde. Homeostatic model assessment of β-cell function and insulin resistance was lower in LG than in CG, whereas homeostatic model assessment of β-cell function and insulin secretory function was higher in LG than in CG. There was a strong positive association between miR-33 level and TG, or TC and or LDL-C, and a strong negative association between miR-33 level and HDL-C. The levels of miR-33 had negative relation with ABCA1 and positive relation with SREBF1. ABCA1 has negative relation with TG, TC, and LDL-C and positive relation with HDL-C. Inversely, SREBF1 had positive relation with TG, TC, and LDL-C and negative relation with HDL-C. The main bioactive compound LBLP-IV of LBL increased insulin secretion of beta cells and the levels of ABCA1, and reduced miR-33 levels and SREBF1 in beta cells. However, LBLP-IV could not change the levels of these molecules anymore when miR-33 was overexpressed or silenced. LBLP-IV had the similar effects with LBL on beta cells while other components had no such effects. Thus, LBLP-IV from LBL improves lipid profiles by upregulating ABCA1 and downregulating SREBF1 via miR-33.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Polysaccharide IV from Lycium barbarum L. Improves Lipid Profiles of Gestational Diabetes Mellitus of Pregnancy by Upregulating ABCA1 and Downregulating Sterol Regulatory Element-Binding Transcription 1 via miR-33

Yang

Fan

et al. 2018

Front. Endocrinol.

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“…The microwave power, extraction time, and ratio of water to raw material are significant parameters to affect the extraction yield of polysaccharides [16]. The effects of microwave power, ratio of water to raw material, and extraction time on the yield of KPS are shown in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Han

Liu

et al. 2019

Molecules

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In order to evaluate effects of extraction techniques on the physicochemical characteristics and antioxidant activities of kiwifruit polysaccharides (KPS), and further explore KPS as functional food ingredients, both microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) were optimized for the extraction of KPS. Furthermore, the physicochemical structures and antioxidant activities of KPS extracted by different techniques were investigated. The optimal extraction conditions of UAE and MAE for the extraction of KPS were obtained by response surface methodology. Different extraction techniques significantly affected the contents of uronic acids, molecular weights, molar ratios of constituent monosaccharides, and the degree of esterification of KPS. Results showed that KPS exhibited remarkable DPPH and ABTS radical scavenging activities, and reducing power. The high antioxidant activities observed in KPS extracted by the MAE method (KPS-M) might be partially attributed to its low molecular weight and high content of unmethylated galacturonic acid. Results suggested that the MAE method could be a good potential technique for the extraction of KPS with high antioxidant activity, and KPS could be further explored as functional food ingredients.

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“…Polysaccharides are the most important group of substances in the goji berry, which are estimated to comprise 5–8% of the dried fruits [ 20 ], 1.02–2.48% of the raw material [ 21 , 22 , 23 ]. More than 40 polysaccharides, with a molecular weight range of 8–241 kDa, were isolated from the fruit of L. barbarum, L. chinense and L. ruthenicum.…”

Section: Constituentsmentioning

confidence: 99%

“…Sevage method and organic reagents were used to remove proteins, pigments and other impurities. The structural composition of a LBP can be studied by SDS-PAGE gel electrophoresis, high perfomance size exclusion chromatography (HPSEC), gas-chromatographic–mass-spectrometry (GC-MS), nucleic magnetic resonance (NMR), and matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-Tof-MS) [ 18 , 21 , 25 ].…”

Section: Constituentsmentioning

confidence: 99%

Systematic Review of Chemical Constituents in the Genus Lycium (Solanaceae)

et al. 2017

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The Lycium genus is widely used as a traditional Chinese medicine and functional food. Many of the chemical constituents of the genus Lycium were reported previously. In this review, in addition to the polysaccharides, we have enumerated 355 chemical constituents and nutrients, including 22 glycerogalactolipids, 29 phenylpropanoids, 10 coumarins, 13 lignans, 32 flavonoids, 37 amides, 72 alkaloids, four anthraquinones, 32 organic acids, 39 terpenoids, 57 sterols, steroids, and their derivatives, five peptides and three other constituents. This comprehensive study could lay the foundation for further research on the Lycium genus.

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Simultaneous determination of molecular weights and contents of water-soluble polysaccharides and their fractions from Lycium barbarum collected in China

Cited by 65 publications

References 29 publications

RETRACTED: Polysaccharide IV from Lycium barbarum L. Improves Lipid Profiles of Gestational Diabetes Mellitus of Pregnancy by Upregulating ABCA1 and Downregulating Sterol Regulatory Element-Binding Transcription 1 via miR-33

RETRACTED: Polysaccharide IV from Lycium barbarum L. Improves Lipid Profiles of Gestational Diabetes Mellitus of Pregnancy by Upregulating ABCA1 and Downregulating Sterol Regulatory Element-Binding Transcription 1 via miR-33

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Systematic Review of Chemical Constituents in the Genus Lycium (Solanaceae)

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