Simultaneous determination of mono-, di-, oligo- and polysaccharides via planar chromatography in 4 different prebiotic foods and 60 naturally degraded inulin samples

Kirchert, Simone; Morlock, Gertrud E.

doi:10.1016/j.chroma.2018.07.042

Cited by 10 publications

(2 citation statements)

References 26 publications

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“…The ammonium acetate buffer did not generate a better zone resolution when compared with water. The addition of 2-aminoethyl diphenylborinate or boric acid that usually improves the resolution of glucose and fructose [65] was also investigated. However, it did not generate a benefit for the separation of steviol glycosides, except for slightly increasing hR F values with increasing additions (ESM Fig.…”

Section: Development Of the Hi-hptlc-uv/vis/fld-hrms Methodsmentioning

confidence: 99%

HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods

Morlock

Heil

2020

Anal Bioanal Chem

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Food products and botanicals on the global market need to be investigated in a more comprehensive way to detect effects, falsifications or adulterations. This is especially true for such ones containing Stevia leaves, Stevia extracts, or steviol glycosides. A multi-imaging profiling was developed exploiting hydrophilic interaction liquid chromatography (HILIC). A minimalistic sample preparation, different mixtures of acetonitrile and water/buffer on the silica gel phase as well as derivatization reagents and optional hyphenation with high-resolution mass spectrometry were exploited. The hydrophilic interaction high-performance thin-layer chromatography (HI-HPTLC) development took 10 min for 48 analyses. It was used to screen Stevia leaf extracts and 20 different food products. For the first time, the biological and biochemical profiling of Stevia leaf products by HI-HPTLC-UV/ Vis/FLD-assay pointed to 19 different bioactive compound bands found in the more natural multicomponent Stevia leaf extracts, whereas most of these activities were not existent for the steviol glycosides. The chemically isolated, purified, and EU-regulated steviol glycosides ease risk assessment and food product development. However, multipotent botanicals may have subtle impact on homeostasis via several metabolic pathways, providing benefits for the consumer's health. Analyzed side by side by means of the effect-directed profiling, their individual activity profiles were visualized as image and individual substances of importance were pointed out. Multi-imaging (comprehensive detection) plus non-targeted bioprofiling (focus on known and unknown bioactivity) allows for a fast detection of questionable product changes that occur along the global food chain and are particularly related to food safety.

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Section: Development Of the Hi-hptlc-uv/vis/fld-hrms Methodsmentioning

confidence: 99%

HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods

Morlock

Heil

2020

Anal Bioanal Chem

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“…The first proof of concept is exemplarily given for the analysis of a food dye mixture and different water samples. However, many applications and workflows, so far handled with commercial instruments, can be replaced by the new 2LabsToGo system, such as the analysis of food composition, − contaminants, , and residues, as well as the bioprofiling of plants, − foods, ,,, and cosmetics …”

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confidence: 99%

2LabsToGo─Recipe for Building Your Own Chromatography Equipment Including Biological Assay and Effect Detection

et al. 2022

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A complete recipe for building your own chromatography equipment from readily available materials is introduced. It combines sample separation (chemistry laboratory) with biological effect detection (biology laboratory). This hyphenation of two disciplines is necessary for prioritizing important compounds in complex samples. Among the thousands of compounds therein, it is often not clear which compounds are the important ones. On the same separation surface, additional detection of biological effects enables and guides substance prioritization. The newly developed open-source 2LabsToGo system for chemical and biological analysis is completely solvent-resistant and, due to miniaturization, environmentally friendly regarding the consumption of materials. It produces comparable results but is 10 times more compact (26 cm × 31 cm × 34 cm), 10 times lighter (6.8 kg), and 55 times less expensive (€ 1717) than current sophisticated commercial devices. As a proof of concept of the first 2LabsToGo system, the quality of different water samples was analyzed since clean water is becoming increasingly rare. In water, most of the thousands of substance signals or features can neither be identified nor classified toxicologically. However, methods that exploit this hyphenated strategy provide answers to such essential safety issues. Drinking or tap water did not show bioactive or toxic compounds, which was expected, whereas biogas or landfill water samples did. The hyphenated 2LabsToGo strategy is affordable and extremely useful for all laboratories with limited equipment but pressing challenges. It is ready to be used in various analytical tasks and applications.

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Ethylamine as new derivatization reagent differentiating reducing from non-reducing saccharides

Schwack,

Heilmann,

Morlock

2023

JPC-J Planar Chromat

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Typical derivatization reagents for saccharides in high-performance thin-layer chromatography, like 2-naphthol sulfuric acid, aniline diphenylamine orthophosphoric acid, or p-aminobenzoic acid, generally detect both reducing and non-reducing saccharides. A new reagent was found with ethylamine, specifically reacting with reducing saccharides on normal-phase silica gel plates, resulting in strongly fluorescent zones after heating the plate at 150 °C for 15 min. In contrast, non-reducing saccharides generally did not reveal fluorescent signals tested with 26 different saccharides. Optimal chromatographic separation was achieved with a mixture of 2-propyl acetate, methanol, and water with 1 mg/mL natural product reagent A when the plate was twofold developed. The high sensitivity of the ethylamine derivatization was shown with mean limits of detection and quantification of 10 and 30 ng per zone, respectively, calculated by different methods for selected mono- and disaccharides. The developed method has exemplarily been used for the digestion control of starch by α-amylase, the determination of lactose in lactose-free milk, and for the quantitative and qualitative study of honey. The analysis of honey gave an excellent example of the advantageous consecutive derivatization with ethylamine and aniline diphenylamine orthophosphoric acid reagent as reagent sequence to detect the coelution of reducing and non-reducing saccharides.

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Simultaneous determination of mono-, di-, oligo- and polysaccharides via planar chromatography in 4 different prebiotic foods and 60 naturally degraded inulin samples

Cited by 10 publications

References 26 publications

HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods

HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods

2LabsToGo─Recipe for Building Your Own Chromatography Equipment Including Biological Assay and Effect Detection

Ethylamine as new derivatization reagent differentiating reducing from non-reducing saccharides

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