Simultaneous determination of morphine, oxycodone, morphine-3-glucuronide, and noroxycodone concentrations in rat serum by high performance liquid chromatography–electrospray ionization–tandem mass spectrometry☆

An automated and fully validated LC‐MS/MS procedure for the simultaneous determination of 11 opioids used in palliative care, with 5 of their metabolites

“…Procedures found in the literature especially focus not only on the morphine-based opioids buprenorphine (BUP), 19 -27 codeine (COD) and morphine (MOR) 28 -38 itself but also on opioids like FEN, 39 -41 hydromorphone (HYD) 42 -44 or methadone (MET). 45 LC-MS methods for analysis of the other opioids of interest like oxycodone (OC), 31,46,47 oxymorphone (OM), 46 piritramide (PIR), 48,49 tilidine (TIL) and tramadol (TRA), 50 -52 and especially assays for the simultaneous determination of different opioids 53 are rarely described. Patients under palliative care often receive combinations of various opioids, e.g.…”

Determination of drugs of abuse and their metabolites in human plasma by liquid chromatography–mass spectrometry

“…Many LC-MS methods for the determination of drugs of abuse in plasma have been published for the determination of opiates [8][9][10][11][12][13][14][15], cocaine and its metabolites [16][17][18][19], amphetamines and designer drugs [20][21][22][23], opiates and cocaine [24][25][26] and opiates, cocaine and LSD [27]. However, only two methods for the simultaneous determination of opiates, amphetamines, benzoylecgonine and cocaine based on LC-MS/MS in oral fluid analysis [28][29][30] and in urine analysis [31] have been published.…”

Potentiation of Morphine Analgesia by Adjuvant Activation of 5-HT7 Receptors

“…The blood was centrifuged and the serum and whole brain (without the cerebellum) were stored at −80°C until analysis. Morphine and its metabolite M3G were extracted from plasma (100 μl) and brain homogenate (0.4 mol/l perchloric acid containing 0.1% ascorbic acid and 0.1% EDTA, 0.3 -0.4 g of tissue/500 μl) and quantified by reversed-phase high-performance liquid chromatography (HPLC) -electrospray ionization -tandem mass spectrometry LC -ESI -MS -MS, according to previously described procedures (9). Deuterated (d3) analogues of each analyte were used as internal standards (plasma: 0.125 ng/μl d3-morphine and 0.25 ng/μl of d3-M3G; brain homogenates: 0.0625 ng/μl of both deuterated compounds).…”