Simultaneous determination of nine flavonoids in beagle dog by HPLC with DAD and application ofGinkgo bilobaextracts on the pharmacokinetic

Abstract

Summary. The method of high-performance liquid chromatography (HPLC) with diode array detector (DAD) was used and validated for the simultaneous determination of nine flavonoids (rutin, myricetin, quercitrin, quercetin, luteolin, genistein, kaempferol, apigenin, and isorhamnetin) in beagle dog plasma. Plasma sample was pre-treated with acetonitrile (containing 0.05% formic acid). Chromatographic separation was performed on a kromasil C 18 column (250 × 4.6 mm, 5 μm) maintained at 35 °C. The mobile phase was a mixture of methanol and 0.2% formic acid with a step linear gradient. At 1.0 mL min −1 flow rate, the eluent of other eight flavonoids was detected simultaneously at 360 nm with good separation except genistein (detected at 254 nm). Under optimum conditions, the correlation coefficient between the peak area and the concentrations for each analyte was all above 0.999. The intra-day and inter-day precisions were less than 10% for all analytes. The limit of detection and the limit of quantification for the selected nine flavonoids were 0.006-0.03 and 0.02-0.12 g mL −1 , respectively. The extracted recoveries of selected nine flavonoids were 74.02%-99.37%. The assay has been successfully applied to determine concentrations of nine flavonoids in plasma from beagle dog after being intravenously administrated Ginkgo biloba extract.

Pharmacokinetic Activity of Quercetin in Rats Following Single Dose Intramuscular Administration

“…The elimination half-life (t 1/2β :0.250.03 h) of Quercetin in rats following single dose intramuscular administration in present study was found lower than reported following intravenous administration as 1.85 h (Khaled et al, 2003) and 3.84 h (Tang et al, 2009) in rats, 1.92 ± 0.62 h in rabbit (Liu et al, 2011) and 3.72 h in dogs (Wu et al, 2012). Whereas, very longer elimination half-life of 36.45± 14.08 h (Yang et al, 2013), 17.70 ± 3.70 h (Erlund et al, 2000) and 13.60±14.09 h in rats (Dong et al, 2017) and 11.90 ± 4.00 h in human (Graefe et al, 2001)has been reported following oral administration.…”

“…Whereas, very longer elimination half-life of 36.45± 14.08 h (Yang et al, 2013), 17.70 ± 3.70 h (Erlund et al, 2000) and 13.60±14.09 h in rats (Dong et al, 2017) and 11.90 ± 4.00 h in human (Graefe et al, 2001)has been reported following oral administration. The apparent volume of distribution (Vd (area) : 132.9224.14 L/kg) of Quercetin following single dose intramuscular administration in rats was found very higher than found 1.16 L/kg in rats (Khaled et al, 2003) and 21.63 L/kg in dogs (Wu et al, 2012) following intravenous administration. Whereas, very low apparent volume of distribution of 14.96  4.44 L/kg (Luo et al, 2016) and 71.70 L/kg (Khaled et al, 2003) in rats following oral administration have been reported.…”

“…Whereas, very low apparent volume of distribution of 14.96  4.44 L/kg (Luo et al, 2016) and 71.70 L/kg (Khaled et al, 2003) in rats following oral administration have been reported. (Khaled et al, 2003) and 9.94 L/h/kg (Tang et al, 2009) in rats, 2.030.22 L/h/kg in rabbits (Liu et al, 2011) and 4.03 L/h/kg in dogs (Wu et al, 2012) following intravenous administration has been reported. Similarly lower body clearance of 0.01 L/h/kg (Khaled et al, 2003) and 1.850.53 L/h/kg (Luo et al, 2016) has been observed in rats following oral administration.…”

Exploring the Anticancer Potential of Traditional Thai Medicinal Plants: A Focus on Dracaena loureiri and Its Effects on Non-Small-Cell Lung Cancer

“…The HPLC analysis of DLEE was performed on a HPLC (Agilent Tecnologies, Santa Clara, CA, USA) using a reversed-phase C18 (250 mm × 4.6 mm, 5 µm) column. The flavonoid compounds (resveratrol, rutin, quercetin, quercitrin, catechin, luteolin, apigenin, hesperetin, and hesperidin) were analyzed using gradient conditions [ 52 ]. The mobile phase composition was established with 0.2% formic acid in water (mobile phase A) and 100% methanol (mobile phase B), initially set at 70% A: 30% B at 0 min.…”