Simultaneous determination of organic acids and iridoid glycosides in traditional Chinese medicine (TCM) preparation reduning injection by rapid resolution liquid chromatography (RRLC) with ultraviolet (UV) detection under segmental monitoring mode

Abstract

A sensitive method of the simultaneous determination of organic acids and iridoid glycoside in reduning injection was described by rapid resolution liquid chromatography (RRLC) with ultraviolet (UV) detection under segmental monitoring mode. RRLC separation was achieved by use of a C 18 analytical column packed with sorbent of particle size 1.8 μm while the injected sample volume was set to 2 µl. A binary gradient elution program of aqueous phosphoric acid (0.1%, v/v) versus acetonitrile was selected for the quantitative analysis of nine major components. The RRLC-UV method under segmental monitoring mode showed satisfactory linearity, the limit of detection and the limit of quantitation. The method had been successfully applied to the determination of nine major components content in reduning injection samples. The analytical time of the proposed protocol is less than that of conventional HPLC-UV analytical methods, thus RRLC-UV method under segmental monitoring mode could function as an attractive alternative method to be used to evaluate the quality of reduning injection in the process of production.

Simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma by UPLC‐MS/MS and its application to a pharmacokinetic study after administration of Reduning injection

“…In China, it is widely used in clinical practice to reduce fever in viral infection diseases, such as serious influenza, infections of the upper respiratory tract and so on (Huang et al, 2006;Gao et al, 2011;Zhang, 2011;Sun et al, 2011). Phenolic acids and iridoid glycosides have been reported to be the principal components of Reduning injection (Zhu et al, 2013;Chang et al, 2013). Pharmacological studies have demonstrated that phenolic acids including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and isochlorogenic acid isomers have outstanding antioxidant, antibacterial, antiviral and antipyretic activity, and iridoid glycosides like geniposide have analgesic and anti-inflammatory effect (Chen et al, 2008;Mishima et al, 2005;Hung et al, 2006;Koo et al, 2006).…”