Simultaneous determination of p-hydroxyacetophenone, chlorogenic acid, and caffeic acid in Herba Artemisiae Scopariae by capillary electrophoresis with electrochemical detection

Abstract

Capillary electrophoresis with electrochemical detection has been employed for the determination of p-hydroxyacetophenone, chlorogenic acid, and caffeic acid in Herba Artemisiae Scopariae (the dried sprout of Artemisia scoparia Waldst. et Kit.). The effects of several important factors, such as the concentration and the acidity of the running buffer, separation voltage, injection time, and detection potential, were investigated to acquire the optimum conditions. The detection electrode was a 300-microm-diameter carbon disc electrode at a working potential of +0.90 V (relative to the saturated calomel electrode). The three analytes can be well separated within 11 min in a 40-cm-long fused-silica capillary at a separation voltage of 15 kV in 50 mM borate buffer (pH 9.2). The relation between peak current and analyte concentration was linear over about 3 orders of magnitude, with detection limits (signal-to-noise ratio of 3) of 0.31, 0.39, and 0.50 microM for p-hydroxyacetophenone, chlorogenic acid, and caffeic acid, respectively. The proposed method has been successfully applied to monitor the three bioactive constituents in real plant samples and to differentiate between different herbal drugs with satisfactory assay results.

Medicinal halophytes: potent source of health promoting biomolecules with medical, nutraceutical and food applications

“…It is also used against inflammation of the liver and cholecystitis (Yamahara et al, 1982;Hikino, 1985). Herba Artemisiae Capillaris is the dried sprout of Artemisia capillaris Thumb, which is a commonly used Chinese herbal drug prescribed for eliminating fever and dampness (Yao and Chen, 2007). Its shape and therapeutic functions are the same as those of Herba Artemisiae Scopariae, although the two herbal drugs come from two different Artemisia species in the family Asteraceae.…”

“…P-hydroxyacetophenone, chlorogenic acid, and caffeic acid are three important cholagogic constituents in Herba Artemisiae Scopariae. They bear a close relation to the quality of the herbal drug and a higher content can indicate a better quality of the crude drugs (Yao and Chen, 2007). P-hydroxyacetophenone has been synthesized and used as a cholagogue (Yao and Chen, 2007).…”

Biomimetic potentiometric sensor for chlorogenic acid based on electrosynthesized polypyrrole

“…It also reduces blood sugar levels and potentially exerts an anti-diabetic effect [26,27]. Thus, the quantitation of CGA in coffee and in plant materials has merited great interest, and a number of methods have been employed for the measurement of CGA based on chromatography [28][29][30], capillary electrophoresis [31][32][33][34] and voltammetry [35][36][37]. Several voltammetric sensors for CGA have likewise been developed [38][39][40], but no potentiometric sensors have yet been described for this analyte.…”

Recent Applications of Capillary Electrophoresis in the Analysis of Traditional Chinese Medicines

“…[46], phydroxyacetophenone, chlorogenic acid and caffeic acid in Artemisia scoparia [47], with detection limits ranging from 0.3 M to 0.52 M. Chen et al [49] described the development and application of a carbon nanotube/poly (ethylene-co-vinyl acetate) composite electrode as a sensitive amperometric detector of CZE. The new ampero-metric detector offered the LODs of 0.22 and 0.27 μM for esculin and esculetin, respectively.…”

“…The development of techniques that can improve detection sensitivity has been a principal area of CZE research. Up to date, electrochemical (EC) [39][40][41][42][43][44][45][46][47][48][49][50][51][52], laser induced fluorescence (LIF) [53,54], electrochemiluminescence (ECL) [55] and MS [56] detections have been successfully applied for CZE analysis of TCM.…”