Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV)

Abstract

During the present study, an endeavor has been made to produce a simple, rapid, and simultaneous method for determination of phenolic compounds by using high-performance liquid chromatography in aerial parts of Leptadenia pyrotechnica (Forssk.) Decne. collected from the Indian Thar Desert. The optimized process was used for the quantification of ten phenolic compounds. The chromatographic separation was accomplished on an Atlantis T3 column at 25°C with isocratic elution. A mixture of acetonitrile and water was used as the mobile phase at a flow rate of 0.8 mL/min. The linear regression examination data for the calibration plots displayed a good linear relationship with r2 > 0.999 in the concentration range of 2–20 µL. In the methanolic extracts of the whole plant of L. pyrotechnica, the content of caffeic acid (3.3%) was reported to be the highest concentration.

Chemodiversity, Biological Activities and Molecular Docking Studies ofLeptadenia pyrotechnica(Forssk.) Decne: A Comprehensive Approach to Validate Its Medicinal Use.

“…This plant is also a component of Ayurvedic drug named as, Jivanti. Almost every plant part has therapeutical potential for different disorders [13] . It is employed in various ways to treat aliments such as plant fiber as antihistaminic and expectorant, whole plant material as antibacterial, plant sap for eczema and other skin disease and leaf paste is applied over the thorn injury.…”

“…The extract of L. pyrotechnica showed antioxidant, anti‐inflammatory, [14] antibacterial, [15] anthelmintic, [16] anti‐lipoxygenase, cytotoxic, [17] and antitumor activities; [18] in addition, it also possesses hypolipidemic & antiatherosclerotic efficacy (in cholesterol fed rabbits) laxative, analgesic, anabolic and astringent, [12] and antioxidant antimicrobial and cytotoxic activities [19] . Previous phytochemical investigations on L. pyrotechnica revealed the presence of some flavonoids, terpenoids, amino acids, sterols, phenolic compounds, fatty alcohols, alkaloids, and pregnane glycosides [13,18] . The present study was intended to validate folk medicinal uses of L. pyrotechnica by assessing the total bioactive content, complete secondary metabolic profile and bioactivities of methanol (LP‐M), ethyl acetate (LP‐E), hexane (LP‐H) and water (LP‐W) extracts.…”

Untitled

“…There are five chief active compounds found in all parts of LP, namely cardiac glycosides (Youssef Moustafa et al, 2009), alkaloids, flavonoids, tannins and saponins (Munazir et al, 2015). The stem contains polyphenolic compounds (Mohammad et al, 2011;Preet and Chand Gupta, 2018), steroids, terpenes, fatty acids (Youssef Moustafa et al, 2007), and other chemical compounds LP is used locally in Saudi Arabia and other Arab countries to add flavor to some foods and as a vegetable. Its stem has been used in traditional medicine to treat several diseases, including tuberculosis (using the water extract) (Patel et al, 2014); smallpox, psoriasis and diabetes (using the sap of young stems) (Verma et al, 2014); dermatitis, kidney disorders, cough, urinary retention, constipation, abortion and cancer (Bhabootra, 2016).…”

Leptadenia Pyrotechnica Aqueous Extract Suppresses Innate Immunity and Enhances Adaptive Immunity in Rats

“…The plant contains many active compounds, such as cardiac glycosides, alkaloids, flavonoids, tannins, and saponins [8]. Additionally, the stem contains polyphenolic compounds [8,9], steroids, terpenes, and fatty acids [8]. In Saudi Arabia, LP is added to meats to add flavor and it is eaten as a vegetable.…”