Simultaneous determination of seven compounds in Lonicera japonica by high performance liquid chromatography

Abstract

A high performance liquid chromatographic (HPLC) method was developed to determine the contents of seven compounds in Lonicera japonica and Folium Lonicerae with maximum wavelength conversion program, and analyze the content differences between them. The separation was performed on an Agilent Eclipse Plus C18 column (250 mm x 4.6 mm, 5 microm) operated at normal temperature with the gradient elution by two mobile phases of water (including 0.3% (v/v) formic acid) (A) and acetonitrile (B) at a flow rate of 1 mL/min. The maximum detection wavelength was set at 330 nm and 350 nm by conversion. The contents of chlorogenic acid and caffeic acid in Lonicera new leaves were 2.572% and 1.498 per thousand respectively, both higher than those cited in Chinese Pharmacopoeia, indicating that Lonicera new leaves have the necessity to be further studied and developed. This method is simple, rapid and highly sensitive. It is suitable for the simultaneous determination of the seven compounds and the quality control of Lonicera japonica.

Simultaneous Quantification of Acid chlorogenic, Acid caffeic and Rutin in Herba Siegesbeckiae orientalis by HLPC Method

“…Qua nghiên cứu các tài liệu về định lượng đồng thời acid chlorogenic (1), acid caffeic (2) và rutin (3) trong dược liệu [11,12], cũng như các đặc điểm hóa lý các chất và phù hợp với điều kiện sẵn có, nghiên cứu lựa chọn cột sắc ký Agilent C18 (250 x 4,6 mm; 5 µm).…”