Simultaneous Determination of Trace Mefenamic Acid in Pharmaceutical Samples via Flow Injection Fluorometry

Abstract

Mefenamic acid belongs to non-steroidal anti-inflammatory drugs that are used widely for the treatment of analgesia. Our aim from this study is to establish a new assay for the quantitative determination of mefenamic acid (MFA) in the pharmaceutical sample by two sensitive and rapid flow injection-fluorometric methods. A homemade fluorometer was used in fluorescence measurements, which using solid-state laser diode 405 and 532 nm as a source, combined with a continuous flow injection technique. The first method depends on the effect of MFA on calcein blue (CLB) fluorescence at 405 nm. Another method is a study of rhodamine-6G (Rh-6G) fluorescence after adding MFA, and recording at 532 nm. Optimum parameters as fluorescent dye concentration, basic medium, flow rate, sample volume, purge time, and delay coil have been investigated. The dynamic range of MFA was 0.2 to 2 mmol.L-1; 0.5 to 2.3 mmol.L-1 with linearity percentage (% r2) 98.92 and 99.83%, for Rh-6G and CLB, respectively. Limit of detection at a minimum concentration in calibration curve 189.34 and 199.89 ng/sample, for Rh-6G and CLB, respectively. The comparison of developed methods with the classical method (UV-vis spectrophotometry) was achieved. The proposed methods were successfully applied for the determination of MFA in the pharmaceutical samples and can be used as an alternative method.

New development method for sulfamethoxazole Analysis in Pharmaceutical formulation using continuous Turbidity Flow Injection Technique

“…Many researchers explain how they used a wide range of analytical techniques to determine the dosage of pure tablets in both the model and the real sample. Analytical methods, such as gas chromatography (GC) and liquid chromatography (LC), are usually applied in Liquid chromatography is the primary method used to separate and analyze compounds in mixtures (7,8,9) . Figure 1.…”

Spectrophotometric determination of amoxicillin in pharmaceutical formulations using normal and reverse flow injection analysis systems: A comparison study

“…The literature covered a variety of analytical techniques [5] for the extraction and determination of AMX, including spectrofluorometry [6,7], electrochemistry [8][9][10], liquid chromatography-mass spectroscopy [11][12][13], highperformance thin layer chromatography (HPTLC) [14], high pressurized liquid chromatography (HPLC) [15], FIA [16][17][18][19][20], magnetic molecularly imprinted polymer [21], and spectrophotometry [22][23][24]. Flow-injection analysis (FIA) techniques are frequently utilized and have generated great interest because of their reproducibility, simplicity, and low apparatus costs [25][26][27][28][29]. The FIA technique is widely used to analyze a broad range of both inorganic and organic substances [30][31][32].…”

Development of an eco‐friendly fluorescent probe for mefenamic acid sensing in pharmaceuticals and biofluids

“…Additionally, it has become necessary to develop simple and sensitive methods to determine its presence in pharmaceutical formulations and biological matrices. Measuring MFA content in different formulations and matrices was reported using chromatographic [8][9][10][11][12][13][14][15], electrochemical [16,17], and spectroscopic [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] methods.…”