Simultaneous determination of uric acid and creatinine in biological fluids by column-switching liquid chromatography with ultraviolet detection

Simultaneous determination of creatinine and uric acid in urine by liquid chromatography–tandem mass spectrometry with polarity switching electrospray ionization

“…These include highperformance liquid chromatography (HPLC) with UV detection [9,10], gas chromatography-mass spectrometry (GC-MS) [11,12], capillary electrophoresis [13], micellar electrokinetic chromatography [14], and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [15][16][17][18][19]. However, no analytical method has been published for simultaneous determination of Cr and UA in urine by LC-MS/MS.…”

On-Line Coupling of Solid Phase Extraction Sample Processing with High-Performance Liquid Chromatography

“…9,10 The direct transfer consisted in discarding the uninteresting parts of a sample initially eluted on a primarily column and in transferring the fraction of interest onto a next column with a minimal of overlapping interferences ( Figure 2). A columnswitching HPLC method applying several different precolumns for the simultaneously determination of uric acid and creatinine in human serum and urine was developed by Selhi et al 11 The fully automated systems where the analytes are extracted on-line with SPE precolumn and separated by HPLC are commercially available from Spark (PROSPECT), [12][13][14] Merck (OSP-2), 15,16 Gilson (ASPEC XL), 17,18 and the precolumns from Waters (Oasis). 19,20 The exchangeable cartridges packed with different type of sorbents could be applied for sample preparation.…”

Simultaneous determination of plasma creatinine, uric acid, kynurenine and tryptophan by high‐performance liquid chromatography: method validation and in application to the assessment of renal function

“…Several high‐performance liquid chromatography (HPLC) methods have been developed for the simultaneous determination of Cr and UA (Seki et al ., ; Yang, ; Zhao, ; Zhiri et al ., ) or KYN and TRP (Iizuka et al ., ; Laich et al ., ; Vignau et al ., ; Zhang et al ., ; Zhao et al ., ) in plasma or serum using a single mobile phase with constant elution. In reverse‐phase HPLC, the volume of organic solvent contained in the mobile phase for the separation of Cr and UA (Zhao, ) was much lower than that for the separation of KYN and TRP (Zhang et al ., ; Zhao et al ., ).…”