Simultaneous determination of vitamin K1, vitamin K1 2,3-epoxide and menaquinone-4 in human plasma by high-performance liquid chromatography with fluorimetric detection

Hirauchi, Kazumasa; Sakano, Toshiyuki; Nagaoka, Tadayoshi; Morimoto, Akira

doi:10.1016/s0378-4347(00)83130-8

Cited by 25 publications

(7 citation statements)

References 11 publications

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“…In agreement with the study of Hirauchi et al (1989a), we found long-chain menaquinones among meat samples only in the liver. The sum of different forms was 2-fold in bovine liver compared to pig liver.…”

Section: Resultssupporting

confidence: 92%

“…The sum of different forms was 2-fold in bovine liver compared to pig liver. Hirauchi et al (1989a) found MK-11 to MK-14 to be the dominant vitamin K forms in bovine liver; in this study, however, these forms were not analyzed at all. As in other studies (Hirauchi et al, 1989a,b;Schurgers et al, 1999) MK-4 was the dominant vitamin K form in different meats (chicken, beef, pork); its concentration was considerably high only in chicken (600 ng/g).…”

Section: Resultscontrasting

confidence: 56%

“…When the fat content of the sample was low (<10%), that was most effectively and easily done with the semipreparative HPLC. In other studies (Hirauchi et al, 1989a;Usui et al, 1989;Udagawa et al, 1993) solid-phase extraction has been used for purification in menaquinone analysis. In preliminary studies both silica and C18 cartridges were tested for purification of bovine liver.…”

Section: Resultsmentioning

confidence: 99%

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Determination of Phylloquinone and Menaquinones in Animal Products with Fluorescence Detection after Postcolumn Reduction with Metallic Zinc

Koivu-Tikkanen

Ollilainen

Piironen

2000

J. Agric. Food Chem.

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A high-performance liquid chromatographic (HPLC) method for the determination of phylloquinone and menaquinones in foods of animal origin is described. The K vitamers were quantified with a fluorescence detector after postcolumn reduction with metallic zinc using K1(25) as an internal standard. Extraction was done either with 2-propanol-hexane (meat and fish products) or with acid hydrolysis method (dairy products). Prior to quantification, sample extracts were purified by semipreparative HPLC; in addition, the fats of cheese and rainbow trout samples were removed with lipase hydrolysis. By this method the phylloquinone and menaquinones (MK-4 to MK-10) present in a few representative samples of different animal food groups were determined. HPLC-MS was used to confirm the identification of K vitamers. Long-chain menaquinones were found from bovine and pig livers as well as from various cheeses. The total vitamin K contents calculated as the sum of quantified K vitamers were in general low (mean content 10-100 ng/g); the highest amount was analyzed in chicken meat (600 ng/g).

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Section: Resultssupporting

confidence: 92%

Section: Resultscontrasting

confidence: 56%

Section: Resultsmentioning

confidence: 99%

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Determination of Phylloquinone and Menaquinones in Animal Products with Fluorescence Detection after Postcolumn Reduction with Metallic Zinc

Koivu-Tikkanen

Ollilainen

Piironen

2000

J. Agric. Food Chem.

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“…Also, no differences were seen in plasma PK and MK homologue concentrations between the hepatocellular carcinoma patients and healthy adults, whose plasma PK and MK homologue concentrations were measured by the same method. 15,16) This indicates that PK and MK homologue contents are especially lower in the cancerous tissue of hepatocellular carcinoma patients.…”

Section: Discussionmentioning

confidence: 94%

“…Measurement of plasma PK and MK homologue concentrations Plasma samples were collected from the 12 patients with hepatocellular carcinoma immediately before surgery and the plasma PK and MK homologue (MK-4, MK-5, MK-6, MK-7, MK-8) concentrations were measured by HPLC combined with coulometric reduction and fluorometric detection, as previously described. 15,16) Statistical analysis A statistical analysis was performed with the Wilcoxon signed-rank test to compare the vitamin K homologue content in the cancerous tissue of hepatocellular carcinoma patients with that of the noncancerous tissue. We used the Mann-Whitney U test to compare the vitamin K homologue content in the cancerous tissue of hepatocellular carcinoma patients with that in the cancerous tissue of metastatic hepatic cancer patients; to compare that in the cancerous tissue of serum PIVKA-II positive hepatocellular carcinoma patients with that in the cancerous tissue of serum PIVKA-II negative patients; and to compare the plasma vitamin K concentration in the serum PIVKA-II positive hepatocellular carcinoma patients with that in the serum PIVKA-II negative patients.…”

Section: Subjectsmentioning

confidence: 99%

Vitamin K Contents in Liver Tissue of Hepatocellular Carcinoma Patients

Miyakawa

Kajiwara²,

Shirahata³

et al. 2000

Japanese Journal of Cancer Research

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Serum protein induced in vitamin K absence-II (PIVKA-II) is used as a tumor marker because it increases at a notably higher rate in patients with hepatocellular carcinoma. To clarify the mechanism causing the elevation of serum PIVKA-II, we measured the contents of vitamins K 1 (phylloquinone, PK) and K 2 (menaquinone, MK) (MK-4, MK-5, MK-6, MK-7, MK-8, MK-9, MK-10) in liver tissue resected from 21 hepatic cancer patients (12 patients with hepatocellular carcinoma and 9 patients with metastatic hepatic cancer), using HPLC combined with coulometric reduction and fluorometric detection. In the cancerous tissue of hepatocellular carcinoma patients, PK, MK-7, MK-8, and MK-10 were significantly lower than that found in the noncancerous tissue. Furthermore, MK-6, MK-7, MK-8, and MK-10 in the cancerous tissue of hepatocellular carcinoma patients were significantly lower than that in the cancerous tissue of metastatic hepatic cancer patients. These data suggested that one of the mechanisms of the elevation of serum PIVKA-II levels in hepatocellular carcinoma patients is a vitamin K deficiency in the local cancerous tissue.

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The external quality assurance of phylloquinone (vitamin K₁) analysis in human serum

Card

Shearer

Schurgers

et al. 2009

Biomedical Chromatography

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The vitamin K external quality assurance scheme (KEQAS) aims to assist in the harmonization of phylloquinone (vitamin K(1)) analysis in order to improve the comparability of clinical and nutritional studies. Serum samples were despatched to 17 groups from eight countries during 2000-2006. Using pilot data (1996-1999), an analytical performance target of 20% absolute difference from the all-laboratory trimmed mean (ALTM) was assigned and formed the basis for interlaboratory comparison. Assay specificity, analytical bias and assay performance were evaluated. From 21 batches of samples distributed, 414 results were reported of which 2.7% were outliers. The mean interlaboratory absolute difference from the ALTM was 21.7% with 47% of groups consistently meeting the performance target. The mean interlaboratory coefficient of variation was 29.6%. The false positive rate for phylloquinone depleted samples was high at 35%. Bias was found to be independent of HPLC-detector type (fluorescence vs electrochemical). Assay characteristics for the measurement of phylloquinone in human serum compare favourably with methods for analytes at equivalent concentrations. The high proportion of false positive results suggest that poor assay specificity at low phylloquinone concentrations is a common problem, which in the clinical setting could lead to underreporting of vitamin K deficiency.

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Simultaneous determination of vitamin K1, vitamin K1 2,3-epoxide and menaquinone-4 in human plasma by high-performance liquid chromatography with fluorimetric detection

Cited by 25 publications

References 11 publications

Determination of Phylloquinone and Menaquinones in Animal Products with Fluorescence Detection after Postcolumn Reduction with Metallic Zinc

Determination of Phylloquinone and Menaquinones in Animal Products with Fluorescence Detection after Postcolumn Reduction with Metallic Zinc

Vitamin K Contents in Liver Tissue of Hepatocellular Carcinoma Patients

The external quality assurance of phylloquinone (vitamin K₁) analysis in human serum

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Simultaneous determination of vitamin K1, vitamin K1 2,3-epoxide and menaquinone-4 in human plasma by high-performance liquid chromatography with fluorimetric detection

Cited by 25 publications

References 11 publications

Determination of Phylloquinone and Menaquinones in Animal Products with Fluorescence Detection after Postcolumn Reduction with Metallic Zinc

Determination of Phylloquinone and Menaquinones in Animal Products with Fluorescence Detection after Postcolumn Reduction with Metallic Zinc

Vitamin K Contents in Liver Tissue of Hepatocellular Carcinoma Patients

The external quality assurance of phylloquinone (vitamin K1) analysis in human serum

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The external quality assurance of phylloquinone (vitamin K₁) analysis in human serum